Phosphorylation of ERK-Dependent NF-κB Triggers NLRP3 Inflammasome Mediated by Vimentin in EV71-Infected Glioblastoma Cells

Enterovirus 71 (EV71) is a dominant pathogenic agent that may cause severe central nervous system (CNS) diseases among infants and young children in the Asia-pacific. The inflammasome is closely implicated in EV71-induced CNS injuries through a series of signaling pathways. However, the activation pathway of NLRP3 inflammasome involved in EV71-mediated CNS injuries remains poorly defined. In the studies, EV71 infection, ERK1/2 phosphorylation, and activation of NLRP3 are abolished in glioblastoma cells with low vimentin expression by CRISPR/Cas9-mediated knockdown. PD098059, an inhibitor of p-ERK, remarkably blocks the vimentin-mediated ERK1/2 phosphorylation in EV71-infected cells. Nuclear translocation of NF-κB p65 is dependent on p-ERK in a time-dependent manner. Moreover, NLRP3 activation and caspase-1 production are limited in EV71-infected cells upon the caffeic acid phenethyl ester (CAPE) administration, an inhibitor of NF-κB, which contributes to the inflammasome regulation. In conclusion, these results suggest that EV71-mediated NLRP3 inflammasome could be activated via the VIM-ERK-NF-κB pathway, and the treatment of the dephosphorylation of ERK and NF-κB inhibitors is beneficial to host defense in EV71-infected CNS.     

大学生成就动机分析

采用叶仁敏修订的“成就动机量表”（AMS）,研究大连4所不同级别、不同类型高校大学生成就动机的特点,研究结果显示：大学生成就动机的性别及学校方面的因素影响差异不显著,家庭及城乡因素影响差异显著。     

浅析建筑屋面防水渗漏的原因及防治措施

建筑防水在建筑设计和施工中占有十分重要的地位。文章通过对材料、设计、施工、管理四个因素对建筑屋面防水渗漏的原因进行分析,并在此基础上提出相应的防治措施,进而明确建筑屋面防水的重要性。     

Adaptive coordination assemblies based on a flexible tetraazacyclododecane ligand for promoting carbon dioxide fixation

Coordination hosts based on flexible ligands have received increasing attention due to their inherent adaptive cavities that often show induced-fit guest binding and catalysis like enzymes. Herein, we report the controlled self-assembly of a series of homo/heterometallic coordination hosts (Me₄enPd)_2n(ML)_n [n = 2/3; M = Zn(ii)/Co(ii)/Ni(ii)/Cu(ii)/Pd(ii)/Ag(i); Me₄en: N,N,N′,N′-tetramethylethylenediamine] with different shapes (tube/cage) from a flexible tetraazacyclododecane-based pyridinyl ligand (L) and cis-blocking Me₄enPd(ii) units. While the Ag(i)-metalated ligand (AgL) gave rise to the formation of a (Me₄enPd)₄(ML)₂-type cage, all other M(ii) ions led to isostructural (Me₄enPd)₆(ML)₃-type tubular complexes. Structural transformations between cages and tubes could be realized through transmetalation of the ligand. The buffering effect on the ML panels endows the coordination tubes with remarkable acid–base resistance, which makes the (Me₄enPd)₆(ZnL)₃ host an effective catalyst for the CO₂ to CO₃²⁻ conversion. Control experiments suggested that the integration of multiple active Zn(ii) sites on the tubular host and the perfect geometry match between CO₃²⁻ and the cavity synergistically promoted such a conversion. Our results provide an important strategy for the design of adaptive coordination hosts to achieve efficient carbon fixation.

A series of coordination hosts were prepared and their applications in CO₂ fixation were studied.     

过氧二甲酰重排反应的理论研究

用ＵＨＦ／ＡＭ１方法研究过氧二甲酰热排反应的机理,结果表明,由自由基对路径是热重排所循的最侍戏,其速控步骤的活化能为９０．１４５ｋＪ．ｍｏｌ＾－１。     

巴马香猪CYP3A29 mRNA表达的TaqMan定量方法学建立

目的 建立巴马香猪CYP3A29 mRNA表达的TaqMan定量方法.方法 通过RT-PCR、TA克隆等技术制 备CYP3A29-pMD18-T和β-actin-pMD18-T实时荧光定量标准品,并以CYP3A29-pMD18-T标准品为模板,对不同浓度Mg2与探针组合的TaqMan反应体系进行筛选,TaqMan PCR方法,并对其有效性及重复性进行评价.结果 筛选到的TaqMan PcR方法为:PCR总体积10 μL,其中含1xBuffer、5.5 mmol/L Mg2、0.8 mmol/L dNTPmix、0.3 μmol/L上下游引物、0.2 U/μL rTaq、0.I μmol/L探针、1 μL模板.热循环条件为94℃5 min→40cycles(94℃15 s→60℃ 45 s→读板).结论 建立了有效测定巴马香猪CYP3A29 mRNA拷贝数浓度的TaqMan PCR方法.通过该方法分别定量CYP3A29和β-actin mRNA的拷贝数浓度,即可求出CYP3A29mRNA的表达水平.     

电子商务客户端安全的研究

随着电子商务规模不断扩大,网上盗窃事件发生率上升.网上窃贼主要利用各种木马病毒和高级的网上钓鱼技术从银行和消费者身上骗取钱财.电子商务交易的安全已成为银行、商家、服务提供者和消费者共同关注的问题.该文以银行和银行客户之间的交易为例,分析电子商务可能存在的各种风险及现有的常见各种欺骗方式,针对客户端目前常见的各种欺诈方式和可能存在的风险提出相应的安全措施,保证客户端安全,实现一个安全的便捷的电子商务交易.     

钢筋混凝土有边框剪力墙抗剪因素分析

依据试验资料论述钢筋混凝土有边框剪力墙的工作机理和强度性能,综合分析剪跨比、配筋率、混凝土强度等因素对剪力墙抗剪强度的影响。     

基于车载激光测距的建筑物立面信息提取

采用车载激光扫描仪等多传感器进行动态测量，各种传感器进行同步数据采集。针对激光扫描仪应用于建筑物立面提取的问题，分析了激光扫描仪数据的特征，建立了建筑物高度提取模型，并开发了相应的立面信息提取系统。     

Convergence of Recursive Identification for ARMAX Process with Increasing Variances

The autoregressive moving average exogenous (ARMAX) model is commonly adopted for describing linear stochastic systems driven by colored noise. The model is a finite mixture with the ARMA component and external inputs. In this paper we focus on a parameter estimate of the ARMAX model. Classical modeling methods are usually based on the assumption that the driven noise in the moving average (MA) part has bounded variances, while in the model considered here the variances of noise may increase by a power of log n. The plant parameters are identified by the recursive stochastic gradient algorithm. The diminishing excitation technique and some results of martingale difference theory are adopted in order to prove the convergence of the identification. Finally, some simulations are given to show the reliability of the theoretical results.