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11.
Based on the complexation between proteins and Cu(II) coupled with the time-resolved chemiluminescence (CL) technique, a highly sensitive and quantitative assay for measuring proteins in solution is described. The complexes of proteins with Cu(II) have a strongly catalytic effect on the luminol-H2O2 CL reaction. Because the CL emission produced by the complexes is much more long-lived than that by Cu(II), the CL signals originating from proteins can be easily identified and measured with a time-resolved technique. On this basis, bovine albumin fraction V (BAF V) can be quantitatively determined in the range of 0.01 - 5.0 microg/ml with a detection limit of 5.8 ng/ml. The results show that the proposed assay exhibits a small variation in the response values for the same amount of different proteins, as compared to the Lowry as well as Bradford assays. The CL assay has also been studied for the detection of immobilized proteins. 相似文献
12.
本文首先提出逆(反)对策这一新问题,给出了数学模型;探讨了“奇门遁甲”预测理论(术)中的数学问题;通过系统分析“专门遁甲”预测过程,可知它的预测过程隐含着一个特殊的逆(反)对策问题;最后指出逆(反)对策问题的广泛存在并给出案例分析. 相似文献
13.
报道了一种弥散型金属薄膜逾渗系统的制备方法和研究结果。从实验发现这种新型的逾渗系统具有异常的R-I关系、三次谐波系数与独特的电流临界规律。分析表明:这些特性与此类薄膜逾渗结构随电流增大而逐渐变化的过程有关,是由沿膜横向逐渐变化的局域隧道电流(LDTC)与跳跃电导(LDHC)效应引起的。 相似文献
14.
Information extraction techniques on the Web are the current research hotspot. Now many information extraction techniques based on different principles have appeared and have different capabilities. We classify the existing information extraction techniques by the principle of information extraction and analyze the methods and principles of semantic information adding, schema defining,rule expression, semantic items locating and object locating in the approaches. Based on the above survey and analysis,several open problems are discussed. 相似文献
15.
To meet the demands of rapid development of pipeline constructions in China, a complete set of full automatic external welding machine for annular welds of pipeline and welding procedure have been developed thereof. The system has been applied in the “West-East Pipeline Project” and its performance is proven. With this welding machine the welding parameters can be either preset before or adjusted in real time during the welding process, which avoids the blemishes of same type domestic and international products. Thus it is best propitious to in-situ construction conditions and workers‘ technical level in China. In this article the development of mechanical and control systems of this product is introduced and typical welding procedure parameters are provided. 相似文献
16.
对传统民法买卖合同风险承担前提的质疑 总被引:2,自引:0,他引:2
传统民法将买卖合同风险承担的前提归结为合同的成立。但是在买卖合同成立后生效前,双方当事人并没有确立确定的债权债务关系,也就谈不上风险承担问题。买卖合同风险承担的前提应为合同的生效,只有在合同生效后,才产生确定的债权债务关系,风险承担才有意义。 相似文献
17.
Zhen‐Feng Chen Hong‐Li Zhou Hong Liang Yan Li Ren‐Gen Xiong Xiao‐Zeng You 《应用有机金属化学》2003,17(11):883-884
The centrosymmetric binuclear structure of [Pb2(H‐Norf)2(ONO2)4]shows the geometry around each lead(II) atom to be distorted trigonal bipyramidal with Pb–O distances ranging from 2.357(3) to 2.769(4) Å. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
18.
通过对师范院校体育教育专业篮球教学目标、教学内容、教学模式、课的结构及对学生的学业成绩评价进行分析和思考 ,提出了当今篮球教学中存在的问题及其解决方法。 相似文献
19.
借助于分子拓扑学探讨了脂肪腈的凝聚型性能与分子结构之间的关系,提出一个结构基础明确的定量关系式.对乙腈到二十一烷腈的计算结果表明,沸点、密度和折光指数的计算值都很接近实验值.应用这一定量关系,不仅能够合理表征脂肪睛结构与凝聚型性能的关系,而且有助于揭示物质结构与性能关系之间的奥秘。 相似文献
20.
The detailed characterization of multifunctional hybrid organosilazane/organosilylamine telechelic oligomers by IR and 1H, 13C and 29Si NMR spectroscopy in one and two dimenions has been undertaken. The specific multifunctional oligomers, comprising NH/NH2 or SiCl functionalities depending on the monomer feed-ratio, were prepared from mono- and di-functionally reacted dichlorodimethylsilane (DDS) and mono-, di-, tri- and tetra-functionally reacted ethylenediamine (EDA). Varying the feed-ratio afforded control of the microstructures of the oligomers and the preparation of oligomers with, in some cases, conterminously located silicon–chlorine (SiCl) groups. The combination of the NMR methods with the IR technique has enabled the detailed microstructural characterization of the oligomers and the identification of the functionalities therein. This approach and the knowledge gained from the DDS/EDA system has been applied to the microstructural characterization of other hybrid organosilazane/silylamine preceramic telechelic oligomers. 相似文献