Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis

To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme (hLYZ) cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400 μg of the p215C3LYZ vector, over 2.0 μg/ml of rhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhLYZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hLYZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis.     

报关舱单及其XML设计

通关速度是国际贸易的要素,报关方式是通关速度的关键。本文以工程资料为背景,详细论述报关舱单采用的W eb报关方式及其捆绑的XML设计。通过对设计原理的分析,可以看出:基于B/S的数据发送方式使报关流程更简洁、作业更开放,因而大大地加快了通关速度,为国民经济的发展做出一点贡献。     

Detecting myocardial ischemia with 2-D CVIB imaging method--an in vivo animal experiment study

A 2-D cyclic variation of integrated backscatter (CVIB) imaging method was established for detecting myocardial ischemia. To demonstrate the feasibility and validity of this method, animal experiments were conducted. Acute myocardial ischemia was induced by occluding left anterior descending coronary artery in 10 anesthetized open-chest dogs. While scanning the normal hearts and the ischemic hearts with a B scanner, digital radiofrequency data were acquired by a real-time acquisition system in synchronism. The offline analysis to the radio-frequency signal with the 2-D CVIB imaging method was performed to verify the consistency between the imaging result and the design of the experiment. In addition, 4 dogs in experiment were treated with the heart pacemaker in order to investigate the influence of changing in heart rate on the detection of ischemic myocardium with the proposed method. The experimental result showed that the 2-D CVIB imaging method succeeded in detecting the ischemic myocardium and is a new non-invasive way for the cardiologists to both quantitatively and visually evaluate the contractile performance of the myocardium.     

固相微萃取膜(SPMEE)在滥用药物检验中的应用

介绍一种全新的SPMEE技术SPMEE法，并对其在滥用药物检验中的应用进行了初步探讨。     

FBE复合覆盖层天然气管道外防腐施工实践

简述了天然气集输设施腐蚀的危害。结合工程实践。介绍了FBE复合覆盖层天然气管道外防腐施工方法及施工工艺，从防腐材料的选择、施工环境温度的控制、涂料的配制等方面对施工技术措施及质量控制要点进行了重点阐述。     

Surface-enhanced Raman scattering study of polymer on metals. III. Chemisorbed polybenzimidazole and its corrosion-inhibiting properties at high temperature

Chemisorption of polybenzimidazole on silver and copper etched with nitric acid has been observed by surface-enhanced Raman scattering (SERS). The polymer is found to react chemically with silver, forming a complexed film on the surface. The SERS spectra show that polybenzimidazole film inhibits oxidation of the metal at high temperature, unlike chemisorbed benzimidazole, benzotriazole, and poly(4-vinyl pyridine). © 1992 John Wiley & Sons, Inc.     

杜邦分析体系与EVA财务管理系统的运用比较

财务管理系统对于公司整体的运营与管理具有举足轻重的作用 .本文针对使用最为广泛的杜邦分析体系和近年来广受关注的EVA财务管理系统 ,比较了两者的异同点 ,分析了它们的优缺点 ,以期望通过对比对建立适合于我国企业实际的财务分析系统有所启示     

有交易费用的衍生物定价模型

该文对考虑交易费费用的衍生物定价方程进行了研究，证明了在一定条件下其可化为线性偏微分方程求解，并得出了具体的定价公式。     

单片机在交流电梯控制系统的应用

本文介绍使用单片机代替交流电梯控制系统中传统使用的继电器控制系统,实现了对层呼信号或指令信号登记、自动定时关门(门联锁)、自动检测定向、顺向载车、停站和自动开门……等十多种功能,提高了运行的平稳可靠、灵活、平层准确以及可调速度、方便维修保养等有关性能.     

板型燃料组件内部流场数值分析

为板型燃料组件的设计和实验研究提供参考 ,采用商用流体力学计算程序 CFX5对板型燃料组件内部流场进行了数值模拟 ,以获得各流速下的板间流场。数值计算结果表明 :板型燃料组件不同流道和单一流道内的流速分布基本均匀 ,在 2 0个流道中居中的 3～ 5流道内的流量比其它流道小一些 ,其中中间流道内的流量最小 ,其流量与平均流量的误差小于 10 % ;提梁对其后部速度场的影响较大 ,对其后部压力场的影响很小。当板间水隙的最大加工误差为0 .15 mm时 ,该流道内的流量与流道内平均流量的误差接近10 %。组件进出口差压计算值与实验值比较接近 ,证明了计算结果的可靠性