基于模糊评价法的景区游客满意度实证研究——以南京中山陵园风景区为例

以著名的5A级景区中山陵园风景区为例,运用模糊评价法测评游客满意度,并做人口统计学特征满意度差异研究.结果显示:游客满意度不高;不同月收入、年龄、文化程度和职业的游客对部分因子的满意度以及总体印象的评价存在显著差异.     

Kinetic 12C/13C isotope fractionation by invertase: evidence for a small in vitro isotope effect and comparison of two techniques for the isotopic analysis of carbohydrates

The natural ¹³C/¹²C isotope composition (δ¹³C) of plants and organic compounds within plant organs is a powerful tool to understand carbon allocation patterns and the regulation of photosynthetic or respiratory metabolism. However, many enzymatic fractionations are currently unknown, thus impeding our understanding of carbon trafficking pathways within plant cells. One of them is the ¹²C/¹³C isotope effect associated with invertases (EC 3.2.1.26) that are cornerstone enzymes for Suc metabolism and translocation in plants. Another conundrum of isotopic plant biology is the need to measure accurately the specific δ¹³C of individual carbohydrates. Here, we examined two complementary methods for measuring the δ¹³C value of sucrose, glucose and fructose, that is, off‐line high‐performance liquid chromatography (HPLC) purification followed by elemental analysis and isotope ratio mass spectrometry (EA‐IRMS) analysis, and gas chromatography‐combustion (GC‐C)‐IRMS. We also used these methods to determine the in vitro ¹²C/¹³C isotope effect associated with the yeast invertase. Our results show that, although providing more variable values than HPLC～EA‐IRMS, and being sensitive to derivatization conditions, the GC‐C‐IRMS method gives reliable results. When applied to the invertase reaction, both methods indicate that the ¹²C/¹³C isotope effect is rather small and it is not affected by the use of heavy water (D₂O). Copyright © 2009 John Wiley & Sons, Ltd.     

基于知识的成卷方法及在MATBAS系统中的实现

本文以 MATBAS(高等数学试题库及计算机辅助命题和评估软件系统)为背号,论述了基于知识的成卷方法。在分析各类考试特点与规律的基础上,提出三类考试模型。在成卷推理中综合使用可信度推理、启发式推理、规划及约束方法。为了使系统控制更灵活,能够生成若干风格不同的等价试卷,还提出了知识层次划分的思想,引入“元知识”的概念,把知识处理结构设计为耦合式的知识库系统。该成卷方法不仅适用于高等数学命题,也适应于别的课程考试。     

高压氢激发态之间的受激喇曼散射

本文采用非稳腔输出的XeCl准分子激光,首次观察到高压氢高激发态之间的受激喇曼散射,并讨论了它们产生的条件.     

光子晶体偏振分光镜的优化设计

提出了一种二维光子晶体偏振分光镜的设计方法,介绍了光栅基板上用Ge/BaF₂ 多层膜实现二维光子晶体偏振分光镜的优化设计.结果表明，TE模式的光子禁带宽度为0083(2πc/a, a是光栅的周期),设计的偏振波长从172μm到24μm，相对带宽为331％.此光子晶体偏振器件制作简单,具有实用性. 关键词： 偏振分光镜 光子晶体 光子禁带     

On-line matrix addition for detecting aerosol particles

Single aerosol particles were measured by matrix-assisted laser desorption/ionization (MALDI) with an aerosol time-of-flight mass spectrometer (ATOFMS). The inlet to the ATOFMS was coupled with an evaporation/condensation flow cell that allowed matrix addition by condensation onto the particles. The coated particles entered the ion source through three-stage differentially pumped capillary inlet and were then ionized by a focused 266 nm Nd:YAG laser. The mass spectra and aerodynamic size of the single particles can be obtained simultaneously. The on-line matrix addition technique makes it possible to identify biological aerosols in real-time.     

Fe(CN)_6~(3-/4-)在铂电极上氧化还原过程的表面拉曼光谱研究(英文)

本文运用共聚焦显微拉曼仪结合特殊的电极表面粗糙方法首次获得了Fe(CN)_6~(3-/4-)在铂电极表面氧化还原过程的拉曼光谱图。研究结果表明Fe(CN)_6~(3-)在电极表面的吸附能力比Fe(CN)~(4-)强,它们都是通过CN上的N原子和电极表面成键而发生化学吸附的。