Effect of lanthanum ions on tRNAphe structure: Imino proton NMR spectroscopy

The effect of lanthanum ions on the structural and conformational change of yeast tRNA Phe was studied by 1H NMR. The results suggest that the tertiary base pair (G-15)(C-48), which was located in the terminal in the augmented dihydrouridine helix (D-helix), was markedly affected by adding La 3+ and shifted 0.33 downfield. Based pair (U-8)(A-14), which is associated with a tertiary interaction, links the base of the acceptor stem to the D-stem and anchors the elbow of the L structure, shifted 0.20 upfield. Another imino proton that may be affected by La 3+ in tRNA Phe is the tertiary base pair (G-19)(C-56). The assignment of this resonance is tentative since it is located in the region of highly overlapping resonances between 12.6 and 12.2. This base pair helps to anchor the D-loop to the TΨC loop.     

Carbon isotope anomaly in marbles associated with eclogites from the Dabie Mountains

This is a report of carbon isotope anomaly in marbles associated with the UHP eclogites from the Dabie Mountains. The results place constraints on the degree of crust-mantle interaction during the UHP metamorphism.     

射流携带床气化炉内混合过程的数值模拟

采用ｋ－εＥ模型对射流携带床气化炉内α－萘酚与对氨基苯磺酸重氮盐的偶合竞争串联反应的分隔指数进行了数值模拟,研究了气化炉出口面积,长径比,两股射流动量比对分隔指数的影响。模拟结果表明,ｋ－ε－Ｅ模型由于同时考虑了宏观混合和微观混合的作用,其观测值和实验测试值吻合较好。     

关于二维Navier—Stokes方程的不稳定性

应用分层理论证明了二维Ｎａｖｉｅｒ－Ｓｔｏｋｅｓ方程的ｃ＾２不稳定性,并给出了重要例证分析。     

相似设计方法在微小移动机器人性能分析中的应用

将微小移动机器人放大后,作模型试验,利用相似理论,进行模型的设计,并把模型的试验结果应用到原型上去。     

Topologic configuration of a graphoglyptid and its functional morphologic analysis

Conclusions  

Spectroscopic studies on 2,3,4,5-tetraphenylpyrylium salts with and without silver (I)-bridged structures

Two novel phenylated pyrylium compounds, silver (I)-bridged 2,3,4,5-tetraphenylpyrylium perchlorate (P1) and its silver (I)-free pyrylium ligand (P2) were prepared from 1,2,3,4-tetraphenylcyclopentadiene to examine their spectroscopic behaviors. The UV/vis absorption and fluorescent emission spectra of P1 and P2, measured in three solvents (acetonitrile, dichloromethane and toluene), reveal that the photophysical behaviors are closely related to silver (I) fragment, and strongly dependent on solvent polarity. In polar acetonitrile, P1 displays longer absorption wavelength and much lower fluorescent emission intensity than P2, although they exhibit much similarity in shape. In contrast, in nonpolar toluene, while P2 shows an apparent absorption band at 338 nm, P1 displays a tail-like line without absorption band observed. All the spectra obtained indicate a better coplanarity and a stronger intra-molecular charge transfer in P1 due to the effect of silver (I) fragment. Additionally, the 1H NMR spectra of P1 and P2, which were recorded under the same conditions, indicate that the silver (I) fragment reinforces pyrylium ring's capacity to localize the formal positive charge within the heterocyclic ring.     

示波极谱法测定地质样品中痕量氟

提出了地质样品经半熔法分解后,8-羟基喹啉沉淀干扰组分,过量的8-羟基喹啉用四氯化碳萃取分离,在六次甲基四胺缓冲液中测定Ce(Ⅲ)-ALC-F~-络合吸附波。并对标准物质中F~-进行测定,其测定值与推荐值吻合。方法的RSD为5.6％,检出限为0.6μg·g~(-1)。     

Reactivity of anatase TiO(2) nanoparticles: the role of the minority (001) surface

Methanol adsorption on clean and hydrated anatase TiO(2)(001)-1 x 1 is studied using density functional theory calculations and first principles molecular dynamics simulations. It is found that (i) dissociative adsorption is favored on clean TiO(2)(001) at both low and high methanol coverages; (ii) on the partially hydrated surface, methanol dissociation is not affected by the coadsorbed water and can still occur very easily; (iii) the dissociative adsorption energy of methanol is always larger than that of water under similar conditions. This implies that water replacement by methanol is energetically favored, in agreement with recent experimental observations on colloidal anatase nanoparticles.     

Isoelectric focusing nonporous silica reversed-phase high-performance liquid chromatography/electrospray ionization time-of-flight mass spectrometry: a three-dimensional liquid-phase protein separation method as applied to the human erythroleukemia cell-line

A liquid-phase three-dimensional protein separation method has been developed that is used to separate the cytosolic fraction of a HEL cell lysate via isoelectric focusing (IEF), nonporous silica (NPS) reversed-phase high-performance liquid chromatography (RP-HPLC) and electrospray ionization time-of-flight mass spectrometry (ESI-TOFMS), respectively. Several hundred unique protein molecular weights were observed in a pI range from 4.8 to 8.5 and a mass range from 5 to 85 kDa. Proteins were positively identified by analysis of the pI (+/-0.5 pI units), an intact protein molecular weight (+/-150 ppm), and peptide mass mapping results. Using the molecular weight (MW) and peptide mapping results of identified proteins it was possible to characterize their posttranslational (PTMs) and/or sequence modifications. PTMs were detected on both forms of cytosolic actin, heat shock 90 beta, HINT and alpha-enolase. Sequence modifications or conflicts were observed for beta-and gamma-actin, ATP beta-synthase and heat shock 90 beta. IEF-NPS-RP-HPLC/ESI-TOFMS was used to determine experimental pI, MW and relative hydrophobicity values for each protein detected. This data was used to generate a 2-D pI-MS protein map, where proteins are displayed according to their pI and molecular weight. Protein molecular weight peaks are represented as bands in the 2-D pI-MS image where the gray scale of each band is proportional to the intensity of the protein molecular weight peak. In addition, a third hydrophobicity dimension (%B) was added as the % acetonitrile elution to generate a 3-D pI-MS-%B plot where each protein can be tagged according to three parameters.