Fluorimetric determination of the levels of urinary neopterin and serum thiobarbituric acid reactive substances in the nonagenarians

Twelve self-sustaining nonagenarians, 10 women and two men, aged 94+/-3 years, and eight institutionalised nonagenarians, eight women, aged 91+/-1 year as well as 11 control subjects, seven women and four men, aged 84+/-5 years entered the study. Urinary neopterin, an indicator of systemic immune activation, and serum thiobarbituric acid reactive substances (TBARS), a marker of lipoperoxidation, were determined initially, and collection of the blood and urine samples was repeated at 3-month interval. Neopterin was measured in the urine specimens by reversed-phase high performance liquid chromatography. A C(18) reversed-phase column 3.3x150 mm, 5 mum-diameter packing Separon SGX was used. Potassium phosphate buffer (15 mmol l(-1), pH 6.4) at flow rate of 0.8 ml min(-1) was used as mobile phase. After centrifugation (5 min, 1300xg) and diluting 100 mul of urine specimens with 1.0 ml of mobile phase containing 2 g of disodium-EDTA per litre, a 20 mul sample was injected on a column. Neopterin was identified by its native fluorescence (353 nm excitation, 438 nm emission). Creatinine was determined by Jaffé kinetic reaction after dilution of sample 1:50 (v/v). The concentration of neopterin in urine was expressed as neopterin/creatinine ratio (mumol mol(-1) creatinine). TBARS were determined spectrofluorometrically using LS-5 spectrofluorimeter (excitation wavelength 528 nm, emission wavelength 558 nm) after extraction with n-butanol treatment with thiobarbituric acid. The significance of differences between nonagenarians and control group was examined by ANOVA-Kruskal-Wallis tests, using statistical software NCSS 6.0.21 (Kaysville, UT, 1996). The decision on significance was based on P=0.05. Urinary neopterin was significantly higher in institutionalised compared to self-sustaining subjects and controls (625+/-565 vs. 203+/-63 mumol mol(-1) creatinine, and 198+/-128 mumol mol(-1) creatinine, respectively, P=0.006). The serum TBARS were higher in both groups of nonagenarians (3.23+/-1.16 mumol l(-1) and 2.69+/-0.39 vs. 2.12+/-0.83 mumol l(-1) for the self-sustaining, institutionalised and controls, respectively, P=0.023). We conclude that the fluorimetric determinations of urinary neopterin and serum TBARS can be useful for the monitoring health status in the elderly patients.     

基于数据挖掘技术的交通事故分析与研究

自塔克拉玛干沙漠公路1995年全线通车以来,由于塔里木石油公路特殊的交通环境,交通事故不断发生,并且有逐年增长的趋势,道路交通事故甚至特大恶性交通事故时有发生,对人们的生命财产安全构成了严重的威胁。由于沙漠地区特殊的自然环境条件,道路交通事故系统的复杂性也在逐渐增强,传统的分析、预防方法已呈现其局限性。为克服事故数据库中数据多维、稀疏、不全等的不利影响,有效地识别和发现沙漠环境下的事故数据的新模式及其内在规律,提出我们应用数据挖掘技术全面整理、分析塔里木石油公路的交通事故的思想,并结合数据挖掘实现的方法和应用实例进行具体阐述,以期为塔里木石油公路安全管理提供科学的决策依据。     

具III类功能反应的非自治捕食扩散系统的全局稳定性(英文)

讨论了一类具有扩散率和 III类功能性反应的非自治捕食系统 ,证明了在适当条件下 ,系统是持久的 ,进一步如果系统是周期系统 ,则在一定条件下存在惟一全局稳定的周期解 .     

浅谈建筑CAD的教学与运用

自20世纪70年代以来，一种全新的计算机辅助设计（CAD）技术发展十分迅速，这是一种以计算机图形学为基础，涉及工程分析、数据管理与数据交换、文档处理、以及软件设计等方面的综合性新技术，成为一种用计算机系统辅助人们对产品或工程设计进行显示、修改、输出的一种新的设计方法。80年代初，它已发展成为工程设计界实用的工具；目前它在世界范围内得到了相当广泛的普及和应用。     

课外阅读在汉语教学中的重要性及其方法

语言教学的最终目的是培养学生运用语言交际的能力,要培养学生的语言能力,只能依赖于丰富的语言积累,而语言的积累主要依赖于大量的阅读。大量的阅读仅靠课堂是不够的,这就需要加大学生的课外阅读量。本文就自己的教学实践来谈在汉语教学中如何加大课外阅读量。     

浅谈现代教育技术在汉语课堂中的应用

我国“入世”后，国际交流的范围进一步扩大，人们有更多的机会借助汉语这一语言工具进行对外联络、交往，用汉语进行交际的机会明显增加。社会对各类人才的汉语知识与能力提出了更高的要求，这对我们汉语教学既是一种挑战，也是一种机会。如何激发学生的学习兴趣，怎样活跃课堂气氛，提高学习效率，现代教育技术为我们解决了这一难题。     

ROOM TEMPERATURE LIGHT-THERMO-DETECTOR MADE OF HIGH-Tc SUPERCONDUCTOR

Based on the anisotropic Seebeck tensor coefficients, a light-thermo-radiation detector made of high-T_c superconductor (YBa₂Cu₃O_7-δ) was fabricated, which can function at room temperature. The induced voltaic signals at various modulation frequencies for radiation from He-Ne laser and from a 500 K black body were measured. The noise ratio for detecting He-Ne laser radiation and the D^* for black body were evaluated. Compared with bolometer made of high-T_c superconductor which functions at liquid nitrogen, and with pyroelectric detector, it was found that the advantages of this new device are that the device can work at room temperature with very low noise and very fast response. Although having a lower D^* value than that of bolometer for the present device construction, we point out the possibility and the direction for improving the D^* value, hence the promising prospect of this kind of device.