葡萄糖氧化酶的固定化方法与改进

本文在固定化葡葡糖氧化酶方面,对几种固定化载体和方法做了最佳条件摸索和比较。改进了戊二醛偶联法,尝试用二乙醇胺先保护戊二醛一端,另一端与载体偶联,然后脱去保护基再偶联酶,效果较好。用该法制得固定化酶在所研究的三种方法中效果最好。固定化酶活力回收率比用聚丙烯酰胺包埋法高出一倍多,比用重氮盐共价键合法高出10%左右。     

The Immobilization of Glucose Oxidase and its Improved Method

The best conditions of immobilizing glucose oxidase using different supports and different methods were studied and compared. An improved method of glucose oxidase immobilization by glutar aldehyde was studied. One end of a glutaraldehyde molecule was first protected by die thanolamine and the other end was linked to the carrier. Then the protected end was hydrolysed and glucose oxidase was linked to it. Glucose oxidase immobilized according to the above method has the highest activity. The enzyme activity was two fold higher than the polyacrylamide and 10% higher than the diazo method.     

禾谷炭疽菌中内吞相关蛋白找寻及特征解析

植物病原丝状真菌内吞过程与菌丝细胞的生长、分化以及发育等关系密切。禾谷炭疽菌是一种半活体营养型植物病原丝状真菌,可以侵染玉米、小麦、高粱等禾本科植物而引起炭疽病,前人对其分泌蛋白在致病性过程中作用的研究较多,但对致病性过程中内吞相关蛋白的研究尚未见报道。构巢曲霉中具有DPFxD基序的蛋白发挥着受体介导的内吞信号作用,有助于实现真菌的内吞过程。基于构巢曲霉DPFxD蛋白序列,通过Blastp比对分析和关键词搜索,明确禾谷炭疽菌中存在48个DPFxD蛋白序列,并利用生物信息学分析工具对上述蛋白进行了亚细胞定位、信号肽、二级结构等特征解析,以及对DPFxD蛋白和同源蛋白开展遗传关系分析,明确其与炭疽菌属中C. incanum、C.sublineola、C. tofieldiae具有较高的同源性。研究成果为进一步探究DPFxD蛋白功能奠定理论基础,也为其他炭疽菌内吞作用蛋白研究提供重要的理论指导。     

疏水性多糖的合成及荧光法研究其在水中的自聚集行为

由胆固醇与１,６－已二异氰酸酯反应合成了１,６－已二异氰酸酯胆固醇单脂（Ｉ）,然后通过葡聚糖与（Ｉ）反应合成了具有自聚集性质的葡聚糖,测得葡聚糖主链上每１００个糖环含有３－５个胆固醇。我们采用探针式超声波来制得胆固醇修饰葡聚糖「ＣＨＤ」的水溶液,同时用Ｎ－苯基－α－萘胺「ＰＮＡ」作为荧光探针来研究ＣＨＤ自聚集过程。在实验中我们发现,每一种葡聚糖溶液,都存在着一个临界浓度。在此浓度之上,ＰＮＡ的荧光     

Molecular modeling of the binding mode of chiral metal complexes A- and A-[Co(phen)_2dppz]~(3 ) with B-DNA

Molecular modeling methods have been applied to the structural characterization of the interaction between chiral metal complexes [Co(phen)2dppz]3 (where phen = 1, 10-phenanthroline, dppz = dipyrido[3,2-a: 2', 3'-c]phenazine) and the oligonucleotide (B-DNA fragment). The natures of two kinds of the binding modes, which are currently intense controversy, have been explored. Barton proposed that there is enantio-selective DMA binding by the octahedral complexes and intercalative access by these complexes from the major groove; but Norden suggested that both enantiomers bind extremely strongly to DNA from the minor groove without any noticeable enantio-selectivity. Our results support and extend structural models based upon Norden's studies, and conflict with Barton's model.     

Efficient Cleavage of DNA by the Novel Copper (II) Complex Derived From 2,2'-Pyridil Ligand

Due to the importance of oxidative degradation of nucleic acids in mutagenesis, carcinogenesis, and aging, a large number of chemical and biological investigations have been recently published1. Artificial metallonucleases have been proven to be efficient tools for the footprinting and sequence-specific targeting of nucleic acids1,2. They usually require high concentrations of the complex and of an external reducing agent (such as dithiothreitol, DTT) to form a reactive species in situ, the f…     

铝原子团簇Al5、Al5-和Al5+稳定结构的密度泛函理论研究

采用密度泛函理论的四种方法 :杂化密度泛函B3LYP与B3PW 91、Perdew Wang91交换与相关泛函WP91PW91、局域自旋密度近似SVWN ,研究了Al5、Al5-和Al5+ 团簇的多种可能结构 ,找到了它们稳定的结构与自旋态 ,与已有的理论结果作了比较 ,并计算了Al5-的绝热与垂直电子离解能、Al5的绝热与垂直电离势 ,同有关的实验数据比较 ,符合较好 .同时对四种密度泛函方法的计算结果作了一些比较与讨论     

司帕沙星氯代物的制备及荧光性质研究

司帕沙星在酸性介质中与亚硝酸反应 ,其重氮盐在氯化亚铜存在下 ,继而与氢氯酸反应生成一种较司帕沙星荧光响应值高 1 1 0倍的强荧光物质 ,采用元素分析、红外光谱和质谱对其衍生物的结构进行了表征。结果表明 ,司帕沙星的荧光强度与其喹啉环 5 位上的氨基密切相关 ,本文还对司帕沙星的荧光自猝灭及氯代荧光增敏机理进行了探讨。     

内燃机车柴油机飞车事故的分析及预防

介绍了飞车发生的现象，分析了内燃机车柴油机飞车事故的原因，提出了飞车事故的处理方法和预防措施。     

住宅楼墙体裂缝的原因分析及防治措施

通过分析砖混结构住宅楼在交付使用几年后出现结构裂缝的基本情况、产生的原因，指出只有在深化设计，严把施工质量关，制定可靠的楼板灌浆工艺标准等方面狠下功夫，才能防治裂缝的产生。