Building addressable libraries: the use of electrochemistry for spatially isolating a heck reaction on a chip

Pd(0) was generated at preselected sites on an electrochemically addressable chip and then utilized to effect a Heck reaction. The Pd(0) was confined to the preselected electrodes with the use of allylmethyl carbonate. Unlike most mediated electrochemical reactions, the electrolysis in this case was not used to convert a stoichiometric process into a catalytic one by recycling the metal. Instead, the unique environment of the chip was used to interfere with a catalytic process to make it stoichiometric. This was done to gain spatial control over the reaction. The development of a strategy for conducting Pd(0)-catalyzed reactions on the chips should greatly expand the synthetic chemistry available for building chip-based libraries.     

Determining PPARγ-ligand binding affinity using fluorescent assay with cis-parinaric acid as a probe

Upon the study of small-molecules binding to proteins, the traditional methods for calculating dissociation constants (Kd and Ki) have shortcomings in dealing with the single binding site models. In this paper, two equations have been derived to solve this problem. These two equations are independent of the total concentration or initial degree of saturation of receptor and the activity of the competitive molecule. Through nonlinear fitting against these two equations, Kd value of a probe can be obtained by binding assay, and Ki value of a ligand can be obtained by competitive assay. Moreover, only the total concentrations of receptor([R]t), ligand([L]t) and probe([P]t) are required for the data fitting. In this work, Ki values of some typical ligands of PPARγ were successfully determined by use of our equations, among which the Ki value of PPARγ-LY171883 was reported for the first time.     

Organic Reactions in Ionic Liquids： An Efficient Method for the Synthesis of Phenacyl Esters by Reaction of Carboxylic Acids with α-Bromoacetophenone Promoted by Potassium Fluoride

An efficient method is reported for the synthesis of phenacyl esters by reaction of carboxylic acids with a-bromoacetophenone promoted by potassium fluoride in ionic liquid [Bmim]PF6, the yield of the reaction is almost quantitative and the products are essentially pure.     

Synthesis of Novel Benzoylphenylurea Chitin Inhibitors From Chlorothalonil(Ⅱ)

IntroductionThe insecticidal activity of benzoylphenylurea( BPU ) analogues,discovered in the early1 970′s[1_ 4] ,is connected with the process ofmoult-ing and appears to affect the chitin synthesis of in-sects. The benzoylphenylurea class of insectgrowth regulators( IGRs) ,interfere with chitinsynthesis in target pests,causing death or abortivedevelopment and are considered to be the fourthgeneration of insecticides or novel materials for in-sect control.Benzoylphenylurea type insecticides…     

巢湖主要入湖河道磷的污染现状

为了解巢湖主要入湖河道中总磷的污染状况,进行了为期三年每月一次的污染调查,十五里河、南淝河、双桥河,污染较重,超出了(GH281-1999)《地表水环境质量标准》的V娄标准。污染主要来自工业废水、生活污水、和农业面源。认为只有开展农业面源污染治理,城市环境综合整治,城市环境综合整治,加强和削减农业化肥的使用量,使得入湖河道水质变好,巢湖的富营养化才能得到控制。     

Two Novel Myrinsol Diterpenes from Euphorbia prolifera

Two novel diterpenes,Euphorprolitherin A(1) and Euphorprolitherin B(2), were isolated from the roots of Euphorbia prolifera. Their structures were elucidated on the basis of spectroscopic methods.     

拟合粘度曲线法研究高聚物的长链支化

本文介绍了用计算机试误法拟合粘度曲线,讨论支化参数的方法,本法可以同时确定支化临界分子量和支化程度,在此基础上可以同时得到支化参数及平均分子量、分子量分布.以聚乙烯标准参考物:SRM 1475(线型)、1476(支化物)及镍催化顺式聚丁二烯为对象介绍了上述方法.     

聚丙烯腈表面金属化新方法的研究

以硼氢化钠（ＮａＢＨ４）水溶液还原聚丙烯腈（ＰＡＮ）／金属盐络合膜后，就可得到一种新的高分子表面金属化材料．其导电性能较好，表面电阻约为１０－１～１０Ω／□通过ＵＶ、ＩＲ等分析手段证实了在高聚物和金属离子之间存在络合作用。研究了影响表面电阻的因素。通过电镜和测量还原率、电子探针微区分析，证实了还原过程中存在离子迁移     

Studies of poly-<Emphasis Type="Italic">L</Emphasis>-lysine-starch nanoparticle preparation and its application as gene carrier

Anion starch nanoparticle (StNP) with a diameter of 50 nm was prepared in water-in-oil microemulsion, with soluble starch as raw materials and POCl₃ as crosslinking agent. PLL-StNP was prepared by linking poly-L-lysine (PLL) on the surface of StNP. At the same time, the size of PLL-StNP and its stability in aqueous solution were checked by AFM. The analysis of plasmid DNA binding, DNase I enzymatic degradation, toxicity and transfection were done. We discovered that PLL-StNP may be used as non-virus nanoparticle gene carrier. And we developed the method of preparing PLL-StNP gene carrier and used it in cell transfection. As non-virus gene carrier, PLL-StNP has some advantages, such as large load of DNA, high transfection efficiency, low cell toxicity and biodegradability.