Fluorimetric determination of the levels of urinary neopterin and serum thiobarbituric acid reactive substances in the nonagenarians

Twelve self-sustaining nonagenarians, 10 women and two men, aged 94+/-3 years, and eight institutionalised nonagenarians, eight women, aged 91+/-1 year as well as 11 control subjects, seven women and four men, aged 84+/-5 years entered the study. Urinary neopterin, an indicator of systemic immune activation, and serum thiobarbituric acid reactive substances (TBARS), a marker of lipoperoxidation, were determined initially, and collection of the blood and urine samples was repeated at 3-month interval. Neopterin was measured in the urine specimens by reversed-phase high performance liquid chromatography. A C(18) reversed-phase column 3.3x150 mm, 5 mum-diameter packing Separon SGX was used. Potassium phosphate buffer (15 mmol l(-1), pH 6.4) at flow rate of 0.8 ml min(-1) was used as mobile phase. After centrifugation (5 min, 1300xg) and diluting 100 mul of urine specimens with 1.0 ml of mobile phase containing 2 g of disodium-EDTA per litre, a 20 mul sample was injected on a column. Neopterin was identified by its native fluorescence (353 nm excitation, 438 nm emission). Creatinine was determined by Jaffé kinetic reaction after dilution of sample 1:50 (v/v). The concentration of neopterin in urine was expressed as neopterin/creatinine ratio (mumol mol(-1) creatinine). TBARS were determined spectrofluorometrically using LS-5 spectrofluorimeter (excitation wavelength 528 nm, emission wavelength 558 nm) after extraction with n-butanol treatment with thiobarbituric acid. The significance of differences between nonagenarians and control group was examined by ANOVA-Kruskal-Wallis tests, using statistical software NCSS 6.0.21 (Kaysville, UT, 1996). The decision on significance was based on P=0.05. Urinary neopterin was significantly higher in institutionalised compared to self-sustaining subjects and controls (625+/-565 vs. 203+/-63 mumol mol(-1) creatinine, and 198+/-128 mumol mol(-1) creatinine, respectively, P=0.006). The serum TBARS were higher in both groups of nonagenarians (3.23+/-1.16 mumol l(-1) and 2.69+/-0.39 vs. 2.12+/-0.83 mumol l(-1) for the self-sustaining, institutionalised and controls, respectively, P=0.023). We conclude that the fluorimetric determinations of urinary neopterin and serum TBARS can be useful for the monitoring health status in the elderly patients.     

基于数据挖掘技术的交通事故分析与研究

自塔克拉玛干沙漠公路1995年全线通车以来,由于塔里木石油公路特殊的交通环境,交通事故不断发生,并且有逐年增长的趋势,道路交通事故甚至特大恶性交通事故时有发生,对人们的生命财产安全构成了严重的威胁。由于沙漠地区特殊的自然环境条件,道路交通事故系统的复杂性也在逐渐增强,传统的分析、预防方法已呈现其局限性。为克服事故数据库中数据多维、稀疏、不全等的不利影响,有效地识别和发现沙漠环境下的事故数据的新模式及其内在规律,提出我们应用数据挖掘技术全面整理、分析塔里木石油公路的交通事故的思想,并结合数据挖掘实现的方法和应用实例进行具体阐述,以期为塔里木石油公路安全管理提供科学的决策依据。     

具III类功能反应的非自治捕食扩散系统的全局稳定性(英文)

讨论了一类具有扩散率和 III类功能性反应的非自治捕食系统 ,证明了在适当条件下 ,系统是持久的 ,进一步如果系统是周期系统 ,则在一定条件下存在惟一全局稳定的周期解 .     

浅谈建筑CAD的教学与运用

自20世纪70年代以来，一种全新的计算机辅助设计（CAD）技术发展十分迅速，这是一种以计算机图形学为基础，涉及工程分析、数据管理与数据交换、文档处理、以及软件设计等方面的综合性新技术，成为一种用计算机系统辅助人们对产品或工程设计进行显示、修改、输出的一种新的设计方法。80年代初，它已发展成为工程设计界实用的工具；目前它在世界范围内得到了相当广泛的普及和应用。     

课外阅读在汉语教学中的重要性及其方法

语言教学的最终目的是培养学生运用语言交际的能力,要培养学生的语言能力,只能依赖于丰富的语言积累,而语言的积累主要依赖于大量的阅读。大量的阅读仅靠课堂是不够的,这就需要加大学生的课外阅读量。本文就自己的教学实践来谈在汉语教学中如何加大课外阅读量。