Determination of periodontal pathogens in human oral cavityEI北大核心CSCD

The simultaneous detection of Porphyromonas gingivalis （PG）,Tannerela forsythia （TF）,and Treponema dentinarum（TD）based on multiplex polymerase chain reaction（PCR）and capillary electrophoresis （CE）,and the effect of sampling position were investigated. Results demonstrated that there was a linear relationship between migration time and DNA length when DNA fragments were separated by capillary electrophoresis,and the correlation coefficient was 0.9976. In 0.5% hydroxyethyl cellulose （HEC）,the PCR products of these periodontal pathogens could be separated within 1.6 min. When sampling at different positions in the periodontal pocket,the PCR products were also different. When analyzing PCR products by capillary electrophoresis,the maximum relative deviation of the peak time was 7.4%. Based on fluorescence intensity,it could be inferred that the concentrations of PG,TD and TF in the gingival fluid were 5.13×10-11 ,7.89×10-11 and 4.87×10-11 ng/µμL,respectively. © 2023, Youke Publishing Co.,Ltd. All rights reserved.     

在高寒山区提高优质豆科牧草产量的研究

根据世行贷款四川省安宁河流域农业资源开发项目凉山州商品羊基地建设报告的要求,开展本研究,旨在探讨在海拔3000m 左右的高寒山区提高优质豆科牧草产量的方法和途径,为养羊业提供足够的优质牧草,促进种草养畜业的健康发展,为项目区农户种草养畜脱贫致富,奠定坚实的基础。本次实验时间是1998年5月至1999年4月,实验用种为光叶紫苕(简称苕子)种子。     

48—50支半细毛羔羊早期补饲试验

通过改善饲养管理务件提高家畜生产力,是当今畜牧业生产最受重视的措施之一。本试验通过对半细毛羔羊实行早期补饲,试验结果表明:早期补饲使48—50支半细毛羔羊的日增重、剪毛量、净毛量等主要生产性能均有一定程度的提高。