Recombinant PsbF from Synechococcus sp. PCC 7002 forms β:β homodimeric cytochrome b559

All organisms with oxygenic photosynthesis contain two photosystems:photosystem Ⅰ(PSⅠ)and photo-systemⅡ(PSⅡ),The minimal photosystem Ⅱ particles which are photochemically active contain three subunits:D1,D2 and cytochrome b559 (Cyt b559),The function of Cyt b559 remains unclear,We have successfully overxpressed the psbF gene,encoding the β subunit of Cyt b559,from a marine cyanobacterium Synechoccous sp.PCC 7002 as a fusion gene and obtained a redox-active from of Cyt b559,When the N-terminal GST protein of the fusion gene product was removed with thrombin ,the PsbF protein was still redox-active,suggesting that the recombinant PsbF can form dimer in Escherichia coli.The absorption spectra of either the oxidized from or the reduced form of both GST fusion protein and the purified PsbF dimer and the difference Spectra between the two forms are the same as that of the Cyt b559 isolated from the higher plants .Redox titration analysis of recombinant PsbF showed that the mid-point redox potential of the recombinant Cyt b559 was approximately 50 mV, which is close to the low potential of Cyt b559 ,The results are helpful to the understanding of locatlization and function of Cyt b559 on thylakoid membranes.Ⅰ     

不同发育时期荔枝种子的生理研究

荔枝种子自花后70天发育至成熟，发芽率和活力指数由0升至最大，种由黄色最后变为红褐色，种子浸泡液的电导率和280nm处的紫外吸收值降到最体，胚轴及子叶中总酚的含量和胚轴中的酸性磷酸酶活性测呈上升的趋势，a－淀粉酶活性测不出来，种子含水量下降，但成熟时种子各部分的含水量仍很高。花后91天，荔枝果实尚未成熟，种子发芽率达100％，活力指数近似最大，此后种子的干鲜重变化很小。采用硅胶轻度脱水可以提高荔枝种子的发芽率及活力指数，不同发育时期的荔枝种子耐脱水力有差别，其中以花后84天的耐脱水力较强，花后98天完全成熟的种子耐脱水力弱。     

论多孔介质中流体流动问题的数值模拟方法

简要介绍了多孔介质中流动问题的数值模拟方法及其发展现状,对各种方法的优劣及适用范围进行了评价。对多孔介质－纯液体耦合流动区域的数值模拟方法及发展现状进行了综合对比。多孔介质模型方法可以用于多孔介质中液体流动问题的数值模拟,并且非常适于进行多孔介质－纯液体耦合流动区域的数值模拟。     

猪生长激素cDNA基因的改建

为了将猪生称激素cDNA基因在表达后能一步纯化获得表达产物,对于来自质粒的猪生长激素cDNS基因通过PCR技术进步了改建,进一步的序列分析表明,与GenBank登录的序列不同,改建后的基因不带有突变。     

海面杂波与目标雷达回波的数值模拟

提出了一种广义前后向迭代方法与谱加速算法结合的快速迭代数值方法,计算了电场水平极化与电场垂直极化锥形波入射到有船目标存在的粗糙海面上时海面散射杂波与目标回波,研究了雷达散射回波与某些重要的物理参数如极化、视角（特别是低掠角）和海面风速等因素的关系,给出了相应的计算结果。     

具强近项的中立型差分方程的振动性和渐进性

使用不等式的某些技巧，获得了如下具强迫项的中立型差分方程△(yn yn-τ) QnG(yn-σ)=fnn∈N的所有解振动或当n→∞时趋于零的充分条件，还获得了如下的中立型差分方程△(yn-yn-τ) QnG(yn-σ)=0n∈N的所有解振动的充分条件。     

冲击波作用下粉末颗粒效应及其形成过程

采用两种颗粒尺寸相差悬珠的高速钢粉末进行爆炸烧结机制的研究,发现了明显的“颗粒效应”,烧结过程首先在小颗粒变形量最大的尖角处发生熔化,在冲击波作用下使液体流激烈运动,加速了颗粒的熔化,最后使颗粒相互粘结在一起,达到烧结的目的。     

Hydrothermal Synthesis and Structure Characterization of Compound Zn（Hpydc）2（H2O）（pydc=pyridine—2,5—dicarboxylate）

1 INTRODUCTION The researches in zinc complexes have been rapidly expanding because of their fascinating structural diversity and potential applications as functional materials and bioloenzymes[1~5]. A lot of compounds of different structural types containing zinc metal have been synthesized and reported[6~9]. In these complexes, the multifunction ligand, such as polyamine, polyacid, and so on, were often elected. In order to obtain more compounds having novel structures, recently, we ch…     

含碳铬矿球团的还原性能

本文讨论了温度、还原剂、铬矿粒度以及与还原剂结合形式等因素,对铬铁矿直接还原性能的影响,并进行了多种添加剂对铬矿还原反应影响的实验。通过气体中间物的固-固反应理论,描述了还原过程。实验结果为开发合碳铬矿球团在竖炉型的熔融还原提供了配料造球的理论依据。