基于荧光共振能量转移的核酸适配体传感器检测环丙沙星

建立了一种基于荧光共振能量转移（FRET）的核酸适配体传感器,并用于检测实际水体和牛奶中的环丙沙星（CIP）。为了防止羧基荧光素（FAM）被CIP猝灭,FAM和四甲基罗丹明（TAMRA）分别标记在互补单链DNA(FAM-cDNA)和适配体（TAMRA-APT）,通过DNA杂交发生FRET, TAMRA有效猝灭FAM的荧光。CIP加入后,其与FAM-cDNA发生亲和力竞争反应,CIP与TAMRA-APT形成结构更稳定的CIP/TAMRA-APT复合物,使体系FAM的荧光恢复。在优化条件下,本方法对CIP表现出高灵敏度和高选择性,检测浓度线性范围为0.01～1μmol/L,检出限为6 nmol/L;对实际水样和牛奶的加标回收率为90.4%～113.2%,相对标准偏差为1.8%～11%。该荧光适配体传感器具有成本低、灵敏度高、特异性好等优点,在环境中CIP残留快速检测方面具有良好的应用潜力。     

Structural Optimization of Damping Groove Based on Valve Plate Dynamic Cavitation Response Model

To reduce cavitation occurring on valve plate of piston pump,an optimization design method was introduced to quantitively analyze the accurate relationship between structural jet groove parameters and cavitation. Using the computational fluid dynamics（ CFD） method,the absorbing and discharging processes in piston pump were simulated dynamically. The damping groove＇s structure effects on both jet angle and pressure shock were analyzed visually with a series of different parametrical grooves. By establishing parametrical damping groove model,the piston pumps＇ dynamic analysis was integrated with CFD analysis,experimental design and approximation model, etc. The mathematical model of plunger pressure during oil back period,jet angle and structural parameters of damping groove were established in the form of second-order response surface method（ RSM） model. The damping groove structure of valve plate was optimized on the basis of the RSM model. Test data shows that the anti-cavitation performance of optimized valve plate was obviously improved. And this method provides theoretical foundation for the structure design of damping groove.     

数控系统的自适应鲁棒控制器设计

基于李雅普诺夫稳定理论与动态逆方法，将自适应鲁棒控制与连续监督控制策略应用于数控系统的控制器设计中，用来补偿摩擦非线性、参数不确定性以及模型不确定性的影响，能显增强系统的稳定性和性能鲁棒性。对该方法进行了理论分析并与基于扰动观测器的控制器进行了对比仿真，仿真结果证明了理论分析的正确性。     

Periodic and chaotic oscillations in mutual-coupled mid-infrared quantum cascade lasers

Dynamic states in mutual-coupled mid-infrared quantum cascade lasers (QCLs) were numerically investigated in the parameter space of injection strength and detuning frequency based on the Lang—Kobayashi equations model. Three types of period-one states were found, with different periods of injection time delay τ_inj, 2τ_inj, and reciprocal of the detuning frequency. Besides, square-wave, quasi-period, pulse-burst and chaotic oscillations were also observed. It is concluded that external-cavity periodic dynamics and optical modes beating are the mainly periodic dynamics. The interaction of the two periodic dynamics and the high-frequency dynamics stimulated by strong injection induces the dynamic states evolution. This work helps to understand the dynamic behaviors in QCLs and shows a new way to mid-infrared wide-band chaotic laser.     

基于QualNet的数据链联合仿真应用研究

为更真实地仿真分析数据链系统性能,基于QualNet仿真框架引入联合仿真思想。提出了一种QualNet与Simulink进行联合仿真分析数据链系统的新方法。通过分析QualNet中底层模型,得出其自身模型难以满足数据链仿真精度要求的结论。在此基础上使用Simulink建立相对精确的数据链底层模型。仿真得到上层所需要的数据,实现与Simulink联合仿真数据链系统。仿真结果分析表明,该方法建立的系统模型能够较真实反映底层模型对上层协议的影响,所建立的系统模型具有较高的可信度和较强的灵活性。     

Banach代数中两个元素之和与积的广义Drazin逆

令a,b为具有单位元1的Banach代数A中两个广义Drazin逆的元素,a^d为a的广义Drazin逆.利用Banach代数中的幂等系统考虑两个元素之和与积的广义Drazin逆,在ab²=bab,b^πba²=b^πaba,a^db²=ba^db等条件下给出a,b之和与积的广义Drazin逆的表达式.     

渐变折射率光纤内的光线轨迹

本文从渐变折射率光纤的几何光学近似出发,推导出渐变折射率光纤的三个光线守恒量和光纤端面上的光线微分方程.并给出应用的例子.得到的结果与其它理论方法得到的结果一致.本文提出的方法和结果对光纤折射率剖面的设计有意义.     

典型非圆形入口自激抖动射流燃烧器的研究

通过数值模拟与燃烧实验对三种典型的非圆形入口突扩腔体自激抖动射流燃烧器的流动与燃烧特性进行研究,结果表明:自激抖动射流燃烧器相对于普通直流射流燃烧器的火焰具有更高的扩散率和卷吸率;相对于菱形和王字形突扩腔体自激抖动射流,三角形突扩腔体射流混合最快且湍流强度更大,燃烧室内温度分布均匀性也最好,说明在腔体入口面积一定的情况下,燃烧特性(湍动能、火焰长度、温度场等)与腔体入口截面周长之间不存在简单的单调函数关系.     

电传动推土机牵引性能匹配的变压控制方法

电传动推土机动力总成包括发动机、发电机、超级电容以及轮边驱动电机,其载荷变化范围大且具有突变性的动力学特征,使发电机与电动机难以实现全局功率匹配.针对电传动推土机动态牵引性能匹配这一关键问题,提出基于双DC-DC(直流-直流)变换器的直流母线电压变压控制方法.即在直流母线与发电机之间加入DC-DC变换器,控制发电机的额定电压工作点,实现发动机在额定工况点工作的高效目标;在直流母线与电动机之间加入DC-DC变换器,根据实时载荷所表达的系统状态变矢量调节变压系数,控制发电机与电动机相对于载荷变化的动力学匹配关系,使发电机与电动机在全载荷意义上实现功率匹配,保证其牵引性能的发挥.通过某型号推土机开发过程性能匹配及其系统数字化模型仿真试验,验证了基于双DC-DC变换器的直流母线电压变压控制方法的有效性.