Applications of homemade kit in mutation detection of genes

With the accomplishment of Human Genome Project (HGP), single nucleotide polymorphism (SNP) and mutation detection in human genome are becom-ing a new researching focus. These researches can help us to understand the phenotype diversity of indi-vidual, disease susceptibility and drug resistance of different colonies. Traditional method used for muta-tion detection is slab gel electrophoresis, which re-mains labor-intensive and time-consuming because of the requirement of radioactivity or te…     

从n型硅到RTN超薄SiO2膜的电流传输特性

用卤素钨灯作辐射热源，对超薄(10nm)SiO2膜进行快速热氮化(RTN)，制备了SiOxN，超薄栅介质膜，并制作了Al／n-Si／SiOxNy／Al结构电容样品．研究了不同样品中n型Si到快速热氮化超薄SiO2膜的电流传输特性及其随氮化时间的变化．结果表明：低场时的漏电流很小；进入隧穿电场时，I—E曲线遵循Fowler-Nordheim(F-N)规律；在更高的电场时，主要出现两种情形，其一是I-E曲线一直遵循F-N规律直至介质膜发生击穿，其二是I-E曲线下移，偏离F-N关系，直至介质膜发生击穿．研究表明，I-E曲线随氮化时间增加而上移．文中对这些实验结果进行了解释．     

求解层合板内残余应力的边界单元法

本文导出基于边界元理论的一种数值方法，用以计算正交层合板的温度应力，对于板的平面问题的应力分析，采用格林基本解答做为权函数，导出板的边界积分方程。对于有侧向载荷的板壳，同时采用两种不同的基本解为权函数，建立一组边界积分方程。数值计算结果收敛，和实验结果基本相符，证实了方法的可行性。     

入世后我国反倾销案件激增的原因与对策

近年来，我国产品在国际市场上屡遭反倾销起诉，一定程度上对我国涉及生产和出口产品的企业乃至整个行业的发展造成了冲击。分析了国外对华反倾销的现状及原因，并提出相应的对策以利于我国当前消除贸易障碍、促进经济发展。     

慢回弹聚氨酯发泡体吸声性能研究

对不同泡孔大小、不同厚度慢回弹聚氨酯材料在不同频段下的吸声性能进行实验研究.研究表明,1 300 μm是材料泡孔大小对吸声性能影响的转折点.对于低频声波,泡孔直径为1 300 μm时,吸声性能最好;对中高频声波,吸声效果最差.材料厚度对吸声性能的影响,6 cm为最优值,此时材料的吸声效果最好;材料对不同频段声波的吸收效果也各不相同,频率为1 000 Hz声波的吸收效果最差,此频率是材料吸声性能的低谷.以上研究结果为工程使用提供了有价值的实验依据.     

SAE#2试验台湿式离合器摩擦转矩测试误差分析

为通过摩擦转矩试验准确得到湿式离合器真实传递的摩擦转矩,建立了SAE#2试验台动力学模型,并基于试验台两摩擦副和六摩擦副湿式离合器摩擦转矩测试对动力学模型进行了验证.随后提出了摩擦转矩测试误差系数以表征离合器真实传递摩擦转矩相对于测得转矩的增大程度,并对离合器主动端转动惯量和系统附加惯量对摩擦转矩测试误差系数的影响规律进行了研究.结果表明:转速转矩传感器测得的转矩并不是离合器真实传递的摩擦转矩,而是转速转矩传感器主动部分的惯性转矩,且小于离合器真实传递的摩擦转矩.随着离合器主动端转动惯量的增大,摩擦转矩测试误差系数线性增大;随着系统附加惯量的增大,摩擦转矩测试误差系数逐渐减小且减小速度逐渐减缓.     

响应面法优化连翘内生真菌J8抗氧化活性物质的发酵提取工艺

对连翘内生真菌J8抗氧化活性物质的发酵提取工艺进行优化。通过单因素筛选,结合Plackett-Burman试验确定影响提取工艺的主要因素,以响应面中心组合法(CCD)拟合了最优组合。结果表明,发酵提取的最优工艺为:当乙酸铵浓度为5.855g/L、介质pH值为7.82、料液比为1∶9.35时,ABTS+·清除率预期值为93.31%。将pH值设为7.8,液料比和乙酸铵不变,在此条件下,J8代谢产物的ABTS+·清除率达到90.23%,与预期值接近。此结果对连翘内生真菌J8进一步分离和纯化其抗氧化性物质研究提供了基础资料。     

刚性筏板下群桩基础共同作用实用分析方法

为了分析实际工程中大规模桩筏基础荷载分担及沉降特性,基于桩-土-筏相互作用理论提出了在竖向荷载作用下考虑土体弹塑性的刚性筏板下群桩基础共同作用的实用分析方法.桩-土-筏相互作用包括桩-土、桩-桩、筏-土和桩-筏相互作用,土体弹塑性考虑了土体模量的双曲线型变化特性.通过对不同尺寸条件下桩筏基础现场实测案例进行分析,介绍了计算过程中相应土体参数的取值方法,并基于基础沉降及桩-土荷载分担比实测和计算值对比结果,验证了该计算方法用于分析实际工程中大规模桩筏基础的合理性.同时基于某工程桩基优化分析,其计算结果表明:考虑筏板的荷载分担能显著降低桩基用量,桩数降为原设计1/3左右时,基础沉降并不会显著增加.     

Cu含量对2A14铝合金显微组织和腐蚀性能的影响

为了提升2A14铝合金耐局部腐蚀性能,采用扫描电镜(SEM)、点蚀测试、循环阳极极化曲线(Tafel)和电化学阻抗谱(EIS)等分析测试方法,研究了Cu含量对2A14铝合金锻件显微组织和腐蚀性能的影响.结果表明:随着Cu含量降低,诱导点蚀的Al2Cu和AlCuMgSi残余结晶相体积分数减小,点蚀坑密度显著降低,合金抗点蚀性能和电化学腐蚀性能大幅提高.     

New testing methodology for the quantification of rock crushability: Compressive crushing value (CCV)

Crushing is a size reduction process that plays a key role in both mineral processing and crushing-screening plant design. Investigations on rock crushability have become an important issue in mining operations and the manufacture of industrial crusher equipment. The main objective of this research is to quantify the crushability of hard rocks based on their mineralogical and mechanical properties. For this purpose, the mineralogical, physical, and mechanical properties of various hard rocks were determined. A new compressive crushing value (CCV) testing methodology was proposed. The results obtained from CCV tests were compared with those from mineralogical inspections, rock strength as well as mechanical aggregate tests. Strong correlations were found between CCV and several rock and aggregate properties such as uniaxial compressive strength (UCS), the brittleness index (S₂₀), and aggregate impact value (AIV). Furthermore, the relationship between the mineralogical properties of the rocks and their CCVs were established. It is concluded that the proposed testing methodology is simple and highly repeatable and could be utilized as a pre-design tool in the design stage of the crushing process for rock quarries.