镀金和碳纳米管修饰金电极上吸附态葡萄糖氧化酶比活性的EQCM研究

采用石英晶体微天平(EQCM)技术监测了裸金电极、镀金和碳纳米管修饰金电极上葡萄糖氧化酶(GOD)的吸附过程. 通过EQCM测量吸附固定的GOD质量, 并实时检测酶反应产物H2O2的氧化电量, 求算了各表面上吸附态GOD的比活性(ESAi). 结果表明, 各表面上均可吸附一定的GOD, 且吸附态GOD均有一定的酶活性; 修饰CNTs可增大酶吸附量和酶电极对葡萄糖的响应电流, 但ESAi随CNTs修饰量的增大而降低; Au电极上电镀金后, 酶吸附量和酶电极对葡萄糖的响应电流亦增大, 但ESAi与裸金电极上的基本一致.     

Application of chiral thiazolidine ligands to asymmetric hydrosilation

Seven chiral thiazolidines bound rhodium complexes were synthesized and their catalytic asymmetric hydrosilation properties were investigated It was found through investigation that the configuration of newly formed chiral centre C2 of substituted chiral thiazolidines prepared from L-cysteine or its esters has no effect on the final results of catalytic asymmetric hydrosilation.The direct reason for causing this phenomenon is reported by the present quantitative results for the first time:the rapid racemation of chiral center C2 of chiral thiazolidine ligands takes place under the catalysis of rhodium(Ⅰ) complex [Rh(COD)CI]2     

压电石英晶体在电解质水溶液中的振荡性能研究(Ⅱ)——频率、频移特性

根据两面电极浸入液体中的压电晶体等效电路模型和Pierce振荡器振荡方程的解,得到晶体在电解质溶液中的振荡频率(F_1)与溶液电导率(x)的关系式:F_1=(1/2π)(1/C_1+1/C_2)(1/X_e)[(R_e~2+X_e~2)x~2+2R_ex+1]。以自行设计的Pierce晶体振荡器。研究了晶体和主振电路参数、输入电压及检测池外壳屏蔽接地对AT切9MHz石英晶体在KCl、Na_2SO_4水溶液中的频率、相对于纯水频移随溶液电导率变化的影响规律,结果与上式相符。     

Purification and Characterization of DNA Methylase From HL-60 Cells

A solid leukemia sarcoma has been successfully developed after subcutaneous inoculation of the cultured human promyelocytic leukemia cells (HL-60 cells) into unde mice. The solid leukemia sarcoma is a more plantiful source than the cultured cells for enzymatic study and its growing environment is closer to that of the human body than the cultured cells.We establish an efficient procedure of purifying HL-60 cells DNA methylase which includes: disruption of HL-60 cells by homogenization and sonication, removing the cell fragments and cellular particles by centrifuge and ultracentrifuge (105.000 g); removing endogenous DNA by streptomycin sulfate, salting out by (NH4)2SO4, ion exchange chromatography on DEAE-cellulose (DE-52), gel filtration over Sephadex G-100 column.The DNA methylase from HL-60 cells has been purified 204 fold by this procedure. The purified enzyme shows a single-band on PG-PAGE. A 479-kD molecular weight of this enzyme is measured by PG-PAGE. The enzyme properties of HL-60 DNA methylase     

齐格勒-纳塔催化剂催化苯乙烯淤浆预聚合

通过淤浆预聚对齐格勒-纳塔催化剂催化的苯乙烯预聚动力学规律和预聚条件对预聚物粒子形态的影响进行了系统的研究。结果表明苯乙烯在石油醚中的聚合速率是衰减型的，但随着溶液中甲苯含量的增加聚合速率曲线会出现增长型。聚苯乙烯的粒子形态与溶剂种类和单体浓度有很大关系。较适宜的预聚条件是在石油醚中单体浓度为0．6mol／L、温度为40℃和反映时间问40min。但由于受扩散控制严重，在石油醚中的聚合速率比较慢。     

饵料及日采收率对蒙古裸腹溞培养效果的影响

~~饵料及日采收率对蒙古裸腹溞培养效果的影响@史海东$浙江海洋学院海洋与渔业研究所,浙江省海洋水产研究所!浙江舟山316100 @辛俭$浙江海洋学院海洋与渔业研究所,浙江省海洋水产研究所!浙江舟山316100 @王海岳$岱山县岱西镇人民政府!浙江岱山316200 @毛国民$浙江海洋学院海洋与渔业研究所,浙江省海洋水产研究所!浙江舟山316100 @姚海富$浙江海洋学院海洋与渔业研究所,浙江省海洋水产研究所!浙江舟山316100~~~~~~浙江省科技厅科研计划项目(021102111)…     

高雷诺数情况下钝体绕流的数值模拟

在高雷诺数 (Re=1× 1 0 4)情况下 ,利用流函数———涡量法在二维空间对半圆半椭圆的拼接体 (新月形覆冰导线的截面 )绕流流场的流动结构、涡的变化及气动力特性进行数值模拟 .将模拟过程用于圆柱体和半圆半椭圆拼接体 ,结果与前人的研究结果及试验值相吻合 ,验证了此数值模拟过程的合理性     

一类具有不确定性时滞大系统的鲁棒稳定条件

根据微分方程理论和矩阵指数特性，讨论了一类具有时变时滞线性大系统的稳定性，并导出了具有时滞相关和时滞无关线性系统稳定性的充分条件，然后讨论了此类大系统的鲁棒稳定条件，并与相关文献进行了比较，计算实例表明，所得出的结果改进了现有的研究成果。