HnRNP K and PDI marked response to chemotherapy to human colorectal cancer cells

5‐Fluorouracil has been the chemotherapy agent of first‐choice for colorectal cancer for many years, but since there are no proven predictors of a patient's response to therapy, all patients receive similar treatment. Consequently, identification of biomarkers for therapeutic effect is crucial for the development of novel therapeutic strategies. Two human colorectal cancer cell lines of different metastatic potential (LoVo and SW480) were studied. IC50 of 5‐FU for both cell lines were measured by 3‐(4,5‐dimethy‐lthiazol‐2‐yl)‐2,5‐diphenyltetrazolium assay and validated by cell cycle analysis. Then the cell lines were treated with 5‐FU at IC50 concentration and protein was extracted for 2‐DE. Differential protein spots were examined by MALDI‐TOF/TOF MS. The expression levels of the different proteins were further confirmed by Western blot and immunofluorescence analyses. Eleven proteins were identified. Expression of heterogeneous nuclear ribonucleoprotein K (hnRNP K) in LoVo cells was higher than in SW480 cells, while protein disulfide isomerase (PDI) displayed the opposite trend. After treatment with 5‐FU, the expression of hnRNP K in LoVo decreased more significantly than in SW480, while PDI in SW480 increased more significantly than in LoVo cells. Conclusion: hnRNP K and PDI in the two cell lines have different expression characteristics. The sensitivity to 5‐FU is not consistent in tumor progression. It may assist in development of novel treatment strategies for colorectal cancer metastasis.     

基于CORBA的主体结构框架

多主体系统是求解复杂问题的一个重要途径，而异构主体间的协作是构造多主体系统的基础，文章基于CORBA对象模型，提出一种主体结构框架，并讨论了主体的协作控制机制，通过对高层设计的复用，使多主体系统的开发能够集中于领域问题求解方面，从而降低了系统开发的难度。     

线性调频信号2-D到达角估计的虚拟阵元法

通过原始数据的共轭重排构造了虚拟阵列，利用实际阵列与虚拟阵列的互Wigner-Ville分布建立了新的空间时频分布，并给出了基于子空间投影和L-阵列的线性调频信号2-D到达角估计算法。理论分析和仿真实验证明了算法的有效性。     

植物病毒病化学防治剂的探寻 Ⅺ.三羧酸乙基DHT酯的合成及抗病毒活性

合成了１０个未见文献报道的三羧酸乙基ＤＨＴ的酯类化合物，新化合物的水溶性、脂溶性均较ＤＨＴ好，并有一定抗病毒活性，其中化合物２的活性最高，对ＴＭＶ的抑制百分率达到９０％，优于对比的ＤＨＴ     

离子交换色谱法分离测定磷霉素的研究

分别采用全多孔型硅胶基键合强阴离子交换柱与氨丙基键合硅胶柱，在低波长 ２００ ｎｍ 处检测，分离测定了磷霉素 探讨了流动相条件，如ｐ Ｈ 值、离子强度等对磷霉素保留及分离选择性的影响，优化分离条件，建立了一种测定磷霉素的高效液相色谱新方法方法简便、快速     

HIRFL高频腔体功率耦合匹配实验研究

 介绍了HIRFL主加速器SSC高频腔体功率馈入耦合匹配系统的研究，实验结果表明，通过改变耦合环角度可更好地实现高频腔体和高频发射机之间的匹配，提高了高频腔体电压和高频发射机的工作效率。     

在程序编码中防止缓冲区溢出

缓冲区溢出攻击是各种网络攻击方法中较普遍且危害较严重的一种，文章分析了缓冲区攻击的原理，并从编程角度分析了造成缓冲区溢出的潜在漏洞，最后提出了在程序编写过程中防御缓冲区溢出的方法。     

Banach空间中余弦算子函数生成元的有界性

A是Banach空间X中余弦算子函数C（t),t∈R，和正弦算子函数S（t),t∈R，的生成元。本证明了，对每个f∈C（[0,T];X)，连续函数u,u(t)=∫-tS(t-s)f(s)ds,f∈[0,T]是二阶非齐次0初值问题，u″=Au f的强解的充要条件是：A是空间X中的有界算子。     

PMIMD拥塞控制算法

分析了目前在Internet上占主导地位的AIMD算法，指出了其在保持数据流稳定性上的不足，这一缺点不能适应当前的媒流体文件传输；提出了一种新的算法--PMIMD（比例乘性增加乘性减少）。仿真结果表明，本算法能减少控制的超调量，从而降低传输率的波动。     

红外成像系统性能模型的研究进展

综述了国内外红外成像系统性能模型的状况。在分析静态性能模型和动态性能模型的基础上，分别给出了其基本构想，阐述了几种静态性能模型的优缺点，讨论了动态性能模型的三种模块；目标背景模块，图像处理算法模块和目标工作平台模块。介绍了它们在预测现场性能的应用。展望了性能模型的发展趋势。