PCR芯片和生化微分析系统

介绍了PCR微芯片的最新研究进展,给出了不同结构的PCR芯片设计原理以及特点,介绍由PCR芯片为主要单元的集成微全分析系统的相关研究,同时简要介绍了对PCR的仿真模拟等。     

Speciation analysis of arsenic in traditional Chinese medicines by hydride generation-atomic fluorescence spectrometry.

A method has been described for the determination of arsenic species (arsenite and arsenate) by hydride generation-atomic fluorescence spectrometry (HG-AFS). The experimental conditions that influence the fluorescence intensity and the reduction of arsenic were investigated and optimized, and the influences from foreign ions and their elimination were studied. The detection limit was found to be 79.7 ng L(-1). The proposed method was applied to the determination of arsenic species in water leachate of traditional Chinese medicines with a recovery range of 91.1-109.5%.     

基于cDNA宏阵列的系统聚类分析猪发育阶段的基因表达谱

取杜洛克猪胚胎第33,45,55，65．75天的背最长肌样本．用cDNA Macroarray分析方法和聚类分析技术分析了327个EST在骨骼肌内不同发育阶段的基因表达谱．结果表明有98条EST在不同发育时期显著差异表达．第33天和第45天两阶段基因表达状态相似．第55天和第65天基因表达状态相似．而第75天的基因表达与第55天和第65天两个阶段的基因表达具有相近的聚类关系．表达状态相近，基因功能相似的基因大都被聚类在一起．     

6to4与ISATAP技术相结合的IPv6网络实现

在IPv4向IPv6过渡期间,多种过渡技术可以互相支持、互相组合使用,以达到互利互助。简要介绍了其中2种隧道技术:6to4技术和ISATAP技术,提出了1种基于两者结合的新型实验网络的实现方案。具体讨论了在Linux下如何组建这种新型实验网络平台,详细阐明了如何配置IPv6主机以及双栈路由器,为IPv4过渡到IPv6提供1个可行的方案。     

图的边韧性度

文[1]中,定义图G(V,E)的边韧性度定义为min{(|S|+T(G-S))/(ω(G-S)):S?E(G)},这里,T-(G-S)和ω(G-S)分别表示G-S中最大分支的顶点数和连通分支数.这是一个能衡量网络图稳定性较好的参数,因为它不仅考虑到了图G-S的分支数也考虑到了它的阶数.在以前的工作中,作者得到了边韧性度图的一个充要条件.利用这些结果证明了K-树是严格边韧性度图,并找到了边韧性度与较高阶的边坚韧度和边坚韧度之间的关系.     

航空放射性遥感技术应用研究

航空放射性遥感系统采用航空γ能谱仪,在空中飞行探测地表放射性,能够快速进行大面积环境放射性调查。结合已经开始进行的首次大面积航空放射性遥感调查工作,简要介绍了航空放射性遥感系统构成、核探测γ能谱测量和数据处理技术。对系统转换因子刻度和剥离系数刻度方法进行了讨论,并对初步应用情况进行了总结。     

光斑光强渐变测量通信光纤数值孔径

数值孔径NA(Numerical Aperture)是通信光纤的一个重要光学参数,表征了光纤的集光能力.它与光纤传输系数的计量有密切关系,对通信光纤的传输带宽有很大影响.光纤数值孔径的测量方法有多种,文中设计了一种用于通信光纤数值孔径快速测量的装置,该装置包括光源、刻度旋转台、微调机构及数字仪表等单元,测量基于光斑光强渐变机理,根据仪表显示值在临界点突变可确定被测光纤的数值孔径,实验表明该装置测量稳定可靠,多次测量平均值的重复性优于2%.     

Penta-Coordinate Phosphorus Intermediates Analysis on Phospho-related Proteins

In this paper, based on known crystal structures of square pyramid （SP） and trigonal bipyramid （TBP） penta coordinated phosphorus compounds containing amino acid side chains, such as amino, carboxyl, hydroxyl or thiol, a software for survey the P（5）-structure of phosphorylated proteins was derived. By this software, it was found that 382 of 398 phosphorus related kinases （96%） from current PDB could go through the penta-coordinated phosphorus transition state or intermediate.     

27,28P+28Si总反应截面增强及可能相关的机制

测量了27,28P和相应同中子异位素在28Si靶上的中能反应截面.测得N=12和13同中子异位素的反应截面在Z=15处突然增大.对Z≤14同中子异位素和28P的实验数据结果可以用改进的光学极限近似的Glauber理论很好地描述.28P的反应截面能够用扩大核芯以改进的Glauber理论来解释.但是,用改进光学极限和少体近似的Glauber理论却低估了27P的实验数据.理论分析表明,扩大的核芯加质子晕可能是响应27P+28Si反应截面增强的机制. The reaction cross sections of~(27,28)P and the corresponding isotones on Si target were measured at intermediate energies. The measured reaction cross sections of the N=12 and 13 isotones show an abrupt increase at Z=15. The experimental results for the isotones with Z≤14 and~(28)P can be described well by the modified Glauber theory of the optical limit approach. The enhancement of the reaction cross section for~(28)P could be explained by the modified Glauber theory with an enlarged core. Theoretical ana...