基于壳聚糖膜固定双酶的胆碱传感器的研究

提出了一种基于壳聚糖膜固定辣根过氧化物酶 胆碱氧化酶的胆碱传感器的制备方法。该传感器以电聚合于玻碳电极的硫堇作为电子传递介体,在pH6. 8,外加电压-0. 2V(vs.SCE)条件下,其峰电流与浓度范围 5. 0×10-5 ~3. 0×10-3 mol/L的胆碱呈良好的线性响应;检出限为 1. 0×10-5 mol/L。传感器有良好的选择性和稳定性,使用一月后,仍能保持其初始活性的 80%。     

Aerobic oxidative cyclization under Pd(II) catalysis: a regioselective approach to heterocycles

[chemical reaction: see text]. An efficient Yb(OTf)3-promoted palladium-catalyzed oxidative cyclization of gamma-heteroalkenyl beta-keto amides has been developed. Under simple aerobic condition, a variety of six-, seven-, and eight-membered-ring N- and O-heterocycles were obtained regioselectively in excellent yield.     

Luminescence 3D-ordered porous materials composed of CdSe and CdTe nanocrystals

The luminescence porous materials of CdTe or CdSe nanocrystals (NCs) were prepared by filling the corresponding NCs into the voids of colloidal crystal by co-deposition of polymer beads and NCs. After removing the beads with tetrahydrofuran (THF), the 3D-ordered porous materials of CdTe (or CdSe) NCs were obtained. The wavelength of maximum photoluminescence of the NCs porous material shows obvious red shift compared with their aqueous dispersion. Under the excitation of high-energy electron the porous materials of CdTe and CdSe NCs will emit photons that can be collected to form a cathode luminescence (CL) image.     

用峰鉴别技术反相高效液相色谱法测定火锅汤料中微量吗啡

本文报道了以峰鉴别技术为定性依据的测定火锅汤料中微量吗啡的反相高效液相色谱法。本法采用改良的Ｓｔａｓ－ｏｔｔｏ法分离提取复杂样品中的微量吗啡。由于采用峰鉴别技术，增加了定性参数，大大提高了定性方法的准确性。本法简便、快速。最低检出浓度为０．０４ｍｇ／Ｌ。在数十例样品分析中，与其它仪器分析结果比照，非常符合。     

停止-流动分光光度法研究铕(II)与二甲酚橙快速电子转移反应动力学

用停止-流动分光光度法研究了Eu~(2+)与二甲酚橙(XO)间快速电子转移反应的动力学规律。求得了速控步骤的动力学参数。如反应级数(n=2)、几个不同温度下的速率常数(k_(278 K)=5.7×10~8 L·mol~-·s~(-1), k_(s38 K)=1.01×10~9 L·mol~(-1)·s~(-1))、活化能(E=7.6×10~3 J·mol~(-1)), 以及指前因子(A=1.5×10~(10) L·mol~(-1)·s~(-1)), 并判断出其为溶液中扩散控制型反应。根据实验现象与测试结果, 提出了Eu~(2+)与XO的反应分别在Eu~(2+)相对过量和在XO过量时的反应机理, 并判断了各反应步骤速率常数之间的相对关系。     

Band structure studies on crystalline C60, Ca3C60, and Ca5C60

The three-dimensional EHMO crystal orbital calculations for crystalline C₆₀, Ca₃C₆₀, and Ca₅C₆₀ are reported. The ground states of both undoped solid C₆₀ and partially doped Ca₃C₆₀ are found to be insulating with an indirect energy gap of 1.2 and 0.5 eV, respectively. In contrast, Ca₅C₆₀ forms a metallic conducting phase with a set of three half-filled bands crossing the Fermi level, which is found to be located close to a peak of the density of state. The character of crystal orbitals near the Fermi level for both Ca₃C₆₀ and Ca₅C₆₀ is completely carbonlike. In both cases, the Ca atoms are almost fully ionized and C₆₀ molecules form a stable negative charge state with six to 10 additional electrons. © 1995 John Wiley & Sons, Inc.     

Comparative study on conventional and low-flow nebulizers for arsenic speciation by means of microbore liquid chromatography with inductively coupled plasma mass spectrometry

The performance of conventional and low-flow nebulizer systems with liquid chromatography in differentiating four arsenic species in urine was evaluated. Two low-flow (DIN and MCN) chamber assemblies and a conventional (CFN) nebulizer-spray chamber assembly were compared in the hyphenation of anion-exchange microbore liquid chromatography with inductively coupled plasma mass spectrometry. Under optimal analytical conditions, the detection limits of the four arsenic species were 0.2-0.6 ng ml(-1) for all the nebulizer systems tested. The chromatographic resolution was best in the case of DIN due to its minimal off-column dead volume and superior transport efficiency. Four arsenic species were determined in the certified reference materials NIST SRM 2670E and 2670N.     

C NMR spectra of cyclomercurated ferrocenylimines: Substituent effects and conformations in the ferrocenes

Three series of cyclomercurated ferrocenylimines (2-chloromercurio-ferrocenylimines) have been studied using ¹³C NMR spectroscopy. Good to excellent linear relationships have been found to exist between the chemical shifts of the carbon atoms in the ferrocenyl moiety and normal Hammett substituent constants σ_m and σ_p. The δ values of the iminyl carbon atoms show excellent linear correlations with the σ values. In three series of the ferrocenes, the sensitivity of the carbon atoms to the substituent effect is different, which is discussed in terms of the twist angle between the N-phenyl ring and the C---C=N---C plane. The relative sensitivity of the chemical shifts on different positions in ferrocenyl moiety to the substituent effect has also been presented.     

Synthesis and photoluminescence of single crystals europium ion-doped BaF2 cubic nanorods

In this work, we used the hydrothermal method to synthesize Eu³⁺ ion-doped cubic BaF₂ nanorods, which is a luminescent material. The clubbed structures were well crystallized and exhibited face-centred cubic structures, as indicated by powder X-ray diffraction, scanning electron microscopy, electron diffraction, and transmission electron microscopy. The luminescent properties were studied, and local symmetry surrounding Eu³⁺ ions and electronic transition processes included. The results indicated that Eu³⁺ occupied only one C_4ν site in nanorods.     

Eliminating positively charged lysine epsilon-NH3+ groups on the surface of carbonic anhydrase has no significant influence on its folding from sodium dodecyl sulfate

This study compares the folding of two polypeptides--bovine carbonic anhydrase (BCA) and peracetylated BCA (BCA-Ac(18))--having the same sequence of amino acids but differing by 18 formal units of charge, from a solution containing denaturing concentrations of sodium dodecyl sulfate (SDS). Acetylation of BCA with acetic anhydride converts all 18 lysine-epsilon-NH(3)(+) groups to lysine-epsilon-NHCOCH(3) groups and generates BCA-Ac(18). Both BCA and BCA-Ac(18) are catalytically active, and circular dichroism spectroscopy (CD) suggests that they have similar secondary and tertiary structures. SDS at concentrations above approximately 10 mM denatured both proteins. When the SDS was removed by dialysis, both proteins were regenerated in native form. This study suggests that large differences in the net charge of the polypeptide have no significant influence on the structure, the ability to refold, or the rate of refolding of this protein from solutions containing SDS. This study reinforces the idea that charged residues on the surface of BCA do not guide protein folding and raises the broader question of why proteins have charged residues on their surface, outside of the region of the active site.