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41.
Repo S Paldanius TA Hytönen VP Nyholm TK Halling KK Huuskonen J Pentikäinen OT Rissanen K Slotte JP Airenne TT Salminen TA Kulomaa MS Johnson MS 《Chemistry & biology》2006,13(10):1029-1039
The chicken genome encodes several biotin-binding proteins, including avidin and avidin-related protein 4 (AVR4). In addition to D-biotin, avidin binds an azo dye compound, 4-hydroxyazobenzene-2-carboxylic acid (HABA), but the HABA-binding properties of AVR4 are not yet known. Differential scanning calorimetry, UV/visible spectroscopy, and molecular modeling were used to analyze the binding of 15 azo molecules to avidin and AVR4. Significant differences are seen in azo compound preferences for the two proteins, emphasizing the importance of the loop between strands beta3 and beta4 for azo ligand recognition; information on these loops is provided by the high-resolution (1.5 A) X-ray structure for avidin reported here. These results may be valuable in designing improved tools for avidin-based life science and nanobiotechnology applications. 相似文献
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Vesa Rauhala 《Tetrahedron》2004,60(41):9199-9204
A new short strategy to prepare the spiroketal fragment of calyculins is presented. A novel Seyferth-Gilbert type homologation of hindered lactols to the corresponding alkynes has been achieved for the first time. The spirocyclization was achieved efficiently via a DIHMA (double intramolecular hetero-Michael addition) process of this hindered ynone. The spirocyclization rate is not dependent on the stereochemistry of the alkoxy substituent in the oxolane ring. 相似文献
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We study the geometry of plane domains and the uniform Hölder continuity properties of analytic functions.With 1 Figure 相似文献
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Perez Carrillo A Bonada J Patynen J Valimaki V 《The Journal of the Acoustical Society of America》2011,130(2):1020-1029
This work presents a method for measuring and computing violin-body directional frequency responses, which are used for violin sound synthesis. The approach is based on a frame-weighted deconvolution of excitation and response signals. The excitation, consisting of bowed glissandi, is measured with piezoelectric transducers built into the bridge. Radiation responses are recorded in an anechoic chamber with multiple microphones placed at different angles around the violin. The proposed deconvolution algorithm computes impulse responses that, when convolved with any source signal (captured with the same transducer), produce a highly realistic violin sound very similar to that of a microphone recording. The use of motion sensors allows for tracking violin movements. Combining this information with the directional responses and using a dynamic convolution algorithm, helps to improve the listening experience by incorporating the violinist motion effect in stereo. 相似文献
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Penttinen H Pakarinen J Välimäki V Laurson M Li H Leman M 《The Journal of the Acoustical Society of America》2006,120(6):4052-4063
This paper presents a model-based sound synthesis algorithm for the Chinese plucked string instrument called the guqin. The instrument is fretless, which enables smooth pitch glides from one note to another. A version of the digital waveguide synthesis approach is used, where the string length is time-varying and its energy is scaled properly. A body model filter is placed in cascade with the string model. Flageolet tones are synthesized with the so-called ripple filter structure, which is an FIR comb filter in the delay line of a digital waveguide model. In addition, signal analysis of recorded guqin tones is presented. Friction noise produced by gliding the finger across the soundboard has a harmonic structure and is proportional to the gliding speed. For pressed tones, one end of a vibrating string is terminated either by the nail of the thumb or a fingertip. The tones terminated with a fingertip decay faster than those terminated with a thumb. Guqin tones are slightly inharmonic and they exhibit phantom partials. The synthesis model takes into account these characteristic features of the instrument and is able to reproduce them. The synthesis model will be used for rule based synthesis of guqin music. 相似文献
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Jari Hyväluoma Vesa Niemi Mahesh Thapaliya Eila Turtola Jorma Järnstedt Jussi Timonen 《Transport in Porous Media》2018,121(2):353-368
The lattice Boltzmann method is increasingly utilized in the simulation of flow-induced wall shear stress needed in various applications. In image-based flow simulations, the simulation geometry is usually based on a three-dimensional reconstruction of the true structure of the pore space obtained, for example, by X-ray tomography. The geometry is then given in a voxel-based representation, which complicates an accurate determination of the surface-normal vectors that are necessary in the computation of the wall shear stress. To avoid this problem, we introduce here a method for the determination of surface-normal vectors directly from a greyscale image instead of its segmented binary image version. The proposed method is fast and automatic, and it can be used for an arbitrary pore space geometry provided in a greyscale form by any imaging modality. We show that this method can produce accurate surface-normal vectors even for binary images and that their accuracy is further increased when the original greyscale images are used instead. We compute wall shear stresses for generated benchmark geometries and then demonstrate the utility of the method for soil samples with ‘random’ pores imaged by X-ray tomography. 相似文献
48.
Salla-Maarit Kokkonen Juha Nikkinen Jukka Remes Jussi Kantola Tuomo Starck Marianne Haapea Juho Tuominen Osmo Tervonen Vesa Kiviniemi 《Magnetic resonance imaging》2009
Analysis of resting-state functional magnetic resonance imaging (fMRI) data is based on detecting low-frequency signal fluctuations in functionally connected brain areas. These synchronous fluctuations in resting-state networks have been observed in several studies with healthy subjects. In this study, we explored if independent component analysis (ICA) can be used to localize the sensorimotor area from resting-state fMRI data in patients with brain tumors. Finger-tapping activation task and resting-state blood-oxygenation-level-dependent fMRI data were acquired from 8 patients with brain tumors and 10 healthy volunteers. Sensorimotor task independent components (ICtask) were used to verify resting-state independent components (ICrest) individually. In addition, sensorimotor ICrests were compared between the groups and no significant differences were detected in volume, spatial correlation or temporal correlation. These results show that it is possible to localize a sensorimotor area from resting-state data using ICA in patients with brain tumors. This offers a complementary method for assessing the sensorimotor area in subjects with brain tumors who have difficulties in performing motor paradigms. 相似文献
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