Small Ubiquitin-Like Modifier Protein 3 Enhances the Solubilization of Human Bone Morphogenetic Protein 2 in <Emphasis Type="Italic">E. coli</Emphasis>

Small ubiquitin-like modifier (SUMO) fusion technology is widely used in the production of heterologous proteins from prokaryotic system to aid in protein solubilization and refolding. Due to an extensive clinical application of human bone morphogenetic protein 2 (hBMP2) in bone augmentation, total RNA was isolated from human gingival tissue and mature gene was amplified through RT-PCR, cloned (pET21a), sequence analyzed, and submitted to GenBank (Accession no. KF250425). To obtain soluble expression, SUMO3 was tagged at the N-terminus of hBMP2 gene (pET21a/SUMO3-hBMP2), transferred in BL21 codon+, and ~?40% soluble expression was obtained on induction with IPTG. The dimerized hBMP2 was confirmed with Western blot, native PAGE analysis, and purified by fast protein liquid chromatography with 0.5 M NaCl elution. The cleavage of SUMO3 tag from hBMP2 converted it to an insoluble form. Computational 3D structural analysis of the SUMO3-hBMP2 was performed and optimized by molecular dynamic simulation. Protein-protein interaction of SUMO3-hBMP2 with BMP2 receptor was carried out using HADDOCK and inferred stable interaction. The alkaline phosphatase assay of SUMO3-hBMP2 on C2C12 cells showed maximum 200-ng/ml dose-dependent activity. We conclude that SUMO3-tagged hBMP2 is more suited for generation of soluble form of the protein and addition of SUMO3 tag does not affect the functional activity of hBMP2.     

Phytochemical screening and in-vitro evaluation of pharmacological activities of peels of Musa sapientum and Carica papaya fruit

Aqueous, absolute and 80% ethanolic extract of fruit peels of Musa sapientum and Carica papaya were investigated for their antibacterial activity, measured by disc diffusion method and antioxidant activity, measured by four different methods. Papaya and banana peels were found to contain terpenoids, tannins, alkaloids, saponins steroid, phenols, fixed oils and fats. 80% ethanolic extract of banana peel was found to contain highest total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity but in papaya peel, highest TPC and reducing activity was shown by water extract while, TFC and radical scavenging activity was given by 80% ethanolic extract. In banana, water extract showed highest antibacterial activity against tested bacteria while in case of papaya, absolute ethanolic extract showed highest antibacterial activity. The present study revealed that peels of banana and papaya fruits are potentially good source of antioxidant and antibacterial agents.     

An effective back‐off selection technique for reliable beacon reception in VANETs

In safety‐critical scenarios, reliable reception of beacons transmitted by a subject vehicle is critical to avoid vehicle collision. According to the employed contention window sizes in IEEE 802.11p, beacons are transmitted with a small contention window size. As a result, multiple vehicles contend for the shared channel access by selecting the same back‐off slot. This is a perfect recipe for synchronous collisions wherein reliable beacon delivery cannot be guaranteed for any vehicle. We consider the problem of selecting the back‐off slots from the current contention window to provide reliable delivery of beacons transmitted by a subject vehicle to its neighbors. Given a safety scenario, we propose a Pseudo‐Random Number Generator (PRNG)‐inspired back‐off selection (PBS) technique. The proposed technique works on the hypothesis that synchronous collisions of beacons transmitted by a subject vehicle can be reduced if all its neighbors select different back‐off slots (ie, not the back‐off slot selected by the subject vehicle). The discrete‐event simulations demonstrate that PBS can increase the overall message reception from a subject vehicle, in comparison with the uniform random probability back‐off selection in IEEE 802.11p.     

An Energy-Efficient and Congestion Control Data-Driven Approach for Cluster-Based Sensor Network

Mobile Networks and Applications - In Wireless Sensor Networks (WSNs), a dense deployment of sensor nodes produce data that contain intra-temporal and inter-spatial correlation. To reduce the...     

Multiattribute‐based routing for lifetime maximization in opportunistic mobile social networks

With the advancement in wireless technology and portable devices, smart phones have become one of the belongings of human being. People share their data online, but it has limitations due to transmission range and mobility. Yet opportunistic mobile social network enables users to share data online even if there is no connected path between source and destination. The widespread use of mobile phones equipped with WiFi, Bluetooth, and several other components and contact opportunities among humans bridge the gap between internet available and nonavailable area. In this paper, we have proposed a new routing approach which utilizes both spatial and temporal attributes of user such as probability to meet a particular location and remaining intercontact time between two nodes to select better relay nodes. Generally, users visit different locations such as sports stadium and mall, with varying probability. Users with similar interest form group, and each user has different intercontact time with other users according to their point of interest and visiting pattern. By utilizing multiattributes, different forwarding strategies have been devised for both inter and intragroup routing. The proposed work “point of interest (PoI)‐based routing” is implemented in Opportunistic Network Environment simulator, and the performance is analysed in terms of delivery rate, latency, overhead, goodput, and energy consumed. The simulation results show that PoI diminishes 23% of overhead and yields 24% improvement in goodput over the state‐of‐the‐art protocol. Thus, the simulation results reveal that our proposed work provides the balance between routing performance and resource consumption.     

Correction to: Similar Feed-forward Loop Crosstalk Patterns may Impact Robust Information Transport Across E. coli and S. Cerevisiae Transcriptional Networks

Mobile Networks and Applications - The original version of this article unfortunately contained a mistake in the author group section. Author Preetam Ghosh’s given name was incorrectly...     

Comparative study of maximum power point tracking techniques for hybrid renewable energy system

The power generation demand is increasing day-by-day throughout the world, therefore, the use of hybrid systems becomes a significant solution. The hybrid renewable energy system (HRES) is used for delivering power in various regions in order to overcome intermittence of wind and solar resources. Because of increasing environmental problems, for example, greenhouse gas emission and energy cost have interested novel research into substitute methods in favour of electrical power generation. Maximum Power Point Tracking (MPPT) control method is a vast deal of novel research used for enhancing the efficiency of HRES. The authors have revealed that the hybrid techniques i.e. Global MPPT, fuzzy-neuro systems, Adaptive Neuro-Fuzzy Inference System (ANFIS), Perturbed and Observe (P&O) + Adaptive Neural Network (ANN) etc. can provide best results as compared to other MPPT control methods. This paper offering a state of art review of MPPT control techniques for HRES.     

Wireless Brain Computer Interface for Smart Home and Medical System

Wireless Personal Communications - The number of aged and disabled people has been increasing worldwide. To look after these people is a big challenge in this era. However, scientists overcome the...     

Measurement of Onset of Structural Relaxation in Melt-Quenched Phase Change Materials

Chalcogenide phase change materials enable non-volatile, low-latency storage-class memory. They are also being explored for new forms of computing such as neuromorphic and in-memory computing. A key challenge, however, is the temporal drift in the electrical resistance of the amorphous states that encode data. Drift, caused by the spontaneous structural relaxation of the newly recreated melt-quenched amorphous phase, has consistently been observed to have a logarithmic dependence in time. Here, it is shown that this observation is valid only in a certain observable timescale. Using threshold-switching voltage as the measured variable, based on temperature-dependent and short timescale electrical characterization, the onset of drift is experimentally measured. This additional feature of the structural relaxation dynamics serves as a new benchmark to appraise the different classical models to explain drift.     

Sustainable Green Fog Computing for Smart Agriculture

Wireless Personal Communications - Medical Body Area Networks or MBANs are gaining popularity in healthcare circles because of the convenience they provide to patients and caregivers and assist in...