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排序方式: 共有140条查询结果,搜索用时 31 毫秒
51.
52.
Bio‐Hybrid Tumor Cell‐Templated Capsules: A Generic Formulation Strategy for Tumor Associated Antigens in View of Immune Therapy 下载免费PDF全文
Lien Lybaert Elly De Vlieghere Riet De Rycke Nane Vanparijs Olivier De Wever Stefaan De Koker Bruno G. De Geest 《Advanced functional materials》2014,24(45):7139-7150
For the development of effective anti‐cancer vaccines, tumor associated antigens need to be internalized by antigen presenting cells alongside specific co‐stimulatory signals. Interestingly, relative to soluble antigens, nano‐ and micro‐particulate antigens are much better presented to CD8 T cells, a crucial step in the induction of cytotoxic T cells that can eliminate malignant cells. In this regard, a generic strategy to encapsulate cancer cell derived proteins into a particulate delivery system would be of high interest. Here we present a versatile approach to incorporate cancer cell proteins into polymeric capsules using the cells themselves as templates for layer‐by‐layer assembly of complimentary interacting species. After coating, the cells are killed by hypo‐osmotic treatment leading to bio‐hybrid capsules loaded with cell lysate. Particular focus is devoted in this work on choosing the optimal coating components and conditions to maximize cell membrane integrity during the coating process, minimize pre‐mature protein release and achieve optimal encapsulation of cell lysate upon lysis of the cells. To further underline the generic nature of our approach, we demonstrate that heat shock proteins, important immune‐activators, can be induced and encapsulated into the bio‐hybrid capsules. 相似文献
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Saad Baaj Georges Skandalis Stefaan Vaes 《Transactions of the American Mathematical Society》2005,357(4):1497-1524
We study the Kac cohomology for matched pairs of locally compact groups. This cohomology theory arises from the extension theory of locally compact quantum groups. We prove a measurable version of the Kac exact sequence and provide methods to compute the cohomology. We give explicit calculations in several examples using results of Moore and Wigner.
55.
Derveaux S Stubbe BG Braeckmans K Roelant C Sato K Demeester J De Smedt SC 《Analytical and bioanalytical chemistry》2008,391(7):2453-2467
In the field of medical diagnostics there is a growing need for inexpensive, accurate, and quick high-throughput assays. On
the one hand, recent progress in microfluidics technologies is expected to strongly support the development of miniaturized
analytical devices, which will speed up (bio)analytical assays. On the other hand, a higher throughput can be obtained by
the simultaneous screening of one sample for multiple targets (multiplexing) by means of encoded particle-based assays. Multiplexing
at the macro level is now common in research labs and is expected to become part of clinical diagnostics. This review aims
to debate on the “added value” we can expect from (bio)analysis with particles in microfluidic devices. Technologies to (a)
decode, (b) analyze, and (c) manipulate the particles are described. Special emphasis is placed on the challenges of integrating
currently existing detection platforms for encoded microparticles into microdevices and on promising microtechnologies that
could be used to down-scale the detection units in order to obtain compact miniaturized particle-based multiplexing platforms.
S. Derveaux and B. G. Stubbe contributed equally to this work. 相似文献
56.
We study equivalence relations and II1 factors associated with (quotients of) generalized Bernoulli actions of Kazhdan groups. Specific families of these actions are entirely classified up to isomorphism of II1 factors. This yields explicit computations of outer automorphism and fundamental groups. In particular, every finitely presented group is concretely realized as the outer automorphism group of a continuous family of non stably isomorphic II1 factors. 相似文献
57.
Lavandera I Kern A Ferreira-Silva B Glieder A de Wildeman S Kroutil W 《The Journal of organic chemistry》2008,73(15):6003-6005
Ketones with two bulky substituents, named bulky-bulky ketones, as well as less sterically demanding ketones were successfully reduced to the corresponding optically highly enriched alcohols using a novel identified recombinant short-chain alcohol dehydrogenase RasADH from Ralstonia sp. DSM 6428 overexpressed in E. coli. 相似文献
58.
Dr. Stefaan De Koker Dr. Jiwei Cui Nane Vanparijs Dr. Lorenzo Albertazzi Prof. Johan Grooten Prof. Frank Caruso Prof. Bruno G. De Geest 《Angewandte Chemie (International ed. in English)》2016,55(4):1334-1339
The induction of antigen‐specific adaptive immunity exclusively occurs in lymphoid organs. As a consequence, the efficacy by which vaccines reach these tissues strongly affects the efficacy of the vaccine. Here, we report the design of polymer hydrogel nanoparticles that efficiently target multiple immune cell subsets in the draining lymph nodes. Nanoparticles are fabricated by infiltrating mesoporous silica particles (ca. 200 nm) with poly(methacrylic acid) followed by disulfide‐based crosslinking and template removal. PEGylation of these nanoparticles does not affect their cellular association in vitro, but dramatically improves their lymphatic drainage in vivo. The functional relevance of these observations is further illustrated by the increased priming of antigen‐specific T cells. Our findings highlight the potential of engineered hydrogel nanoparticles for the lymphatic delivery of antigens and immune‐modulating compounds. 相似文献
59.
Release mechanisms for polyelectrolyte capsules 总被引:1,自引:0,他引:1
De Geest BG Sanders NN Sukhorukov GB Demeester J De Smedt SC 《Chemical Society reviews》2007,36(4):636-649
Polyelectrolyte capsules have recently been introduced as new microscopic vehicles which could have high potential in the biomedical field. In this critical review we give an introduction to the layer-by-layer (LbL) technique which is used to fabricate these polyelectrolyte capsules as well as to the different triggers that have been exploited to obtain drug release from these capsules. Furthermore, other types of triggered delivery systems are compared and critically discussed with regard to their clinical relevance. (171 references.). 相似文献
60.