Functional Mechanism of Resveratrol in Inhabiting Growth of Cells ls174t and Its Mechanism in Subcutaneously Transplanted Tumor of Nude Mice

To explore the functional mechanism of Resveratrol against colon cancer cells Is174t and the growth of colon cancer tissue of tumor-bearing mice,MTT method was used to observe the functions of resveratrol for inhibition against cells ls174t in vitro.Transmission electron microscope was used to observe the cell apoptosis.FCM assay was performed to measure the change of the cell apoptosis rate and of cell cycle,RT-PCR method was used to detect the expressions of bc1-2 and bax mRNA.Western blot method was used to detect the expressions of bc1-2 and bax protein.Cells isi74t were transplanted subcutaneously to nude mice to observe the effect of resveratrol on the growth of subcutaneously transplanted tumor.RT-PCR method was used to detect the expressions of bc1-2 and bax mRNA in the tumor tissue.Western blot method was used to detect the expressions of bc1-2 and bax protein in the tumor tissue.Resveratrol has an effect of inhibiting proliferation of cells ls174t in vitro(P<0.01).It is able to induce the apoptosis of cells Is174t,causing the decrease in the expression of bc1-2 and the increase in the expression of bax.Resveratrol could inhibit the growth of subcutaneously transplanted tumor of nude mice(P<0.05),causing the decrease in the expression of bc1-2 and the increase in the expression of bax.Resveratrol can inhibit the growth of cells 174t and the growth of subcutaneously transplanted tumor.The mechanism is possibly related to the induction of the cell apoptosis and the regulation of bc1-2/bax expression.     

单片机在牙齿光学检测中的应用

本文论述了8031单片机在牙齿光学检测系统中所起的作用。并描述了牙齿活力和脱钙的检测方法。这种方法,是通过对牙齿表面磷光或反射光的客观测量来实现的。从而,解决了以前人们曾不能解决的问题。     

牙齿磷光检测技术研究

本文分析了牙齿磷光测量技术一直不能进一步发展的原因,提出了一种新的检测技术。这种技术利用光纤传输紫外光和磷光,利用光电接收器作为传感器,解决了牙齿磷光现象一直不能定量分析的问题,使得牙齿磷光现象与牙齿疾病之间关系的研究得到了进一步的发展。     

Synthesis of the Active Stilbenoids by Photooxidation Reaction of trans-e-Viniferin

Introduction It was reported that stilbene monomers showed multi-faced biological activities, such as antioxidation, antimutagen, antibacterial, antifungal activities.1 Espe-cially, isorhapontigenin and resveratrol showed potent inhibition on biosynthesis of leukotriene and its receptor antagonist.2 Some oligostilbenes exhibited more potent bioactivities than their monomers.3 In recent years, a number of oligostilbenes were isolated from natural sources, but a few of studies on their pharmacol…     

Study on the Intramolecular Cyclization through Openmg of Epoxide IV. Cyclization of N-Methyl-N-benzoylmethylene β-Methyl-α, β- epoxypropionamide in Aprotic and Protic Solvents

Intramolecular cyclization of β -methyl substituted epoxide under aprotics (e.g. LDA in benzene) and protic conditions (e.g. LiOH in MeOH/H2O)both gave a mixture of β-, γ-, δ- and ε-lactam but in different ratio.     

Two New Dihydroflavanoids from Patrinia vittosa Juss (II)

Two new dihydroflavonoids named villosin A and villosin B were obtained from the traditional Chinese medicinal herb Patrinia villosa Juss. Their structures were elucidated as (2S)-5, 2', 6'-trihydroxy-2", 2"-dimethylpyrano [5", 6": 6, 7]flavanone 1 and (2S, 3"S)-5, 2', 6'-trihydroxy-3"-γ, γ-dimethylallyl-2", 2"-dimethyl-3", 4"-dihydropyrano [5", 6": 6, 7] flavanone 2 by spectroscopic methods including UV, IR, HR-TOF- MS, 1D NMR and 2D NMR techniques.     

肝豆状核变性病人血清和尿中铜的测定及其临床意义

本文为拓宽分析仪器在临床医学中的应用 ,建立了肝豆状核变性病人血清和尿中铜的火焰原子吸收光谱测定方法。方法的相对标准偏差小于 1.4 % ,检出限为 7 3× 10 - 3μg·mL- 1 ,加标回收率在 94 %～10 4 %之间。讨论了肝豆状核变性病人血铜和尿铜水平对临床诊断及治疗的意义。实验结果表明 ,血清铜和尿铜水平对于WD虽不具单独诊断意义 ,必须结合临床症状和其他生化指标综合诊断 ,但它们却是诊断本病的重要参考指标 ,尤其在治疗方面 ,及时监控血铜水平和尿铜排出量具有指导意义。本法已广泛应用于临床实际样品的分析 ,获得良好效果。     

红外标准加入法测定复合植物生长剂中三十烷醇的含量

采用红外标准加入法,测定复合植物生长剂中三十烷醇的含量。样品经处理后,在提取的三十烷醇中加入系列标准品,制成溴化钾片,测定含量。该法平均回收率97.3%,测量变异系数2.5%。     

激光与顺铂共同作用下对癌细胞的影响

通过对CNE_2细胞的实验研究,探讨了激光与顺氨氯铂的治癌机理,指出它实质上是一种光敏治疗。     

新化合物阴行草醇的NMR研究

我们从植物阴行草中提取分离到一种新的化合物(1),并将其命名为阴行草醇。利用核磁共振技术辅以其它波谱数据准确无误地确定了该化合物的立体结构,指定了~1H和~(13)C的所有谱线。