Synthesis,Characterization, and X-Ray Structures of 2-(3,5-Dimethylpyrazol-1-Yl)Phenyl-Based Organoselenium Compounds and Their Glutathione Peroxidase (Gpx) Like Activity

Abstract

The synthesis of a series of 2-(3,5-dimethylpyrazol-1-yl)phenyl-based organoselenium compounds, (dmpzC₆H₄Se)₂ (1), dmpzC₆H₄SeR (dmpz = 3,5-dimethylpyrazol-1-yl; R = (CH₂)_nY; Y = OH, NH₂, and COOH), and dmpzC₆H₄SeX (X = Cl, Br, or I) is described. The compounds are characterized by IR, NMR (¹H, ¹³C{¹H}, ⁷⁷Se{¹H}), and mass spectral (MS) data. The molecular structures of (dmpzC₆H₄Se)₂, dmpzC₆H₄SeCH₂COOH, and dmpzC₆H₄SeCH₂CH₂OH have been established by X-ray crystallography. The two latter compounds are associated in the solid state through intermolecular hydrogen bonding between the OH proton and the pyrazolyl nitrogen atom of the adjacent molecule. Glutathione peroxidase (GPx) like catalytic activity of these compounds has been evaluated by using hydrogen peroxide (H₂O₂) as substrate and dithiothreitol (DTT^red) as thiol cofactor in CD₃OD, and the progress of the reaction was monitored by ¹H NMR spectroscopy. All the compounds exhibited the GPx-like catalytic activity. Among these, the ones containing alkylamino groups showed the best activity.

Supplemental materials are available for this article. Go to the publisher's online edition of Phosphorus, Sulfur, and Silicon and the Related Elements to view the free supplemental file.     

Synthetic,Spectroscopic, and Biological Aspects of Triorganosilicon(IV) Complexes of Tridentate Schiff Bases

Abstract

Hexacoordinated organosilicon complexes of type R₃Si(L) (R = ethyl, butyl, phenyl; HL = ligand, obtained by the condensation of 4-aminoantipyrine with 2-hydroxyacetophenone, 2-hydroxybenzophenone, 2-hydroxybenzaldehyde, and 2-hydroxynapthaldehyde) have been synthesized and characterized by elemental analysis, molar conductance, and spectroscopic studies (IR, ¹H, ¹³C, and ²⁹Si NMR). The spectroscopic studies indicated that the ligands acted as tridentate coordinating through azomethine nitrogen, carbonyl oxygen, and oxygen of hydroxyl after deprotonation to the central silicon atom. The ligands and their organosilicon complexes have been evaluated for antimicrobial activities against fungi (Aspergillus niger and Candida albicans), and against Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis), and Gram-negative bacteria (Escherichia coli). The aim of the present work is to synthesize novel eco-friendly fungicides and bactericides and to study the effect of the biological activity of ligands on the complexation with organosilicon moiety. Supplemental materials are available for this article. Go to the publisher's online edition of Phosphorus, Sulfur, and Silicon and the Related Elements to view the free supplemental file.     

Combustion Synthesis and Optical Properties of Eu3+-Doped BaGd2ZnO5 f–f Transition Nanophosphor for White LED

An Eu³⁺-doped BaGd_2(1?x)ZnO₅ nanophosphor has been synthesized by means of a single-step, urea-assisted, solution-combustion process. The structural, morphological, and optical properties of the nanophosphor were studied by x-ray diffraction (XRD), scanning electron microscopy, transmission electron microscopy, and photoluminescence spectroscopy. The XRD results showed that the pure orthorhombic BaGd₂ZnO₅ structure with space group Pbnm was obtained at 900°C. The intense red luminescence at 628 nm on near-UV (396 nm) excitation is because of the hypersensitive ⁵D₀ → ⁷F₂ transition of luminescent activator Eu³⁺ ions, located at a site with no inversion symmetry in the BaGd₂ZnO₅ crystal lattice. The optimum doping concentration and decay time of Eu³⁺-doped BaGd_2(1?x)ZnO₅ nanophosphor were also determined. The emission could be effectively tuned from blue to the white and red regions by varying the concentration of europium ions. Decay curve analysis revealed that cross-relaxation is primarily responsible for the concentration quenching. High luminescent intensity, low-cost, easy synthesis, uniform shape, and controlled color tunability suggest use of BaGd₂ZnO₅:Eu³⁺ as an efficient red-emitting nanophosphor for near-UV-based LED solid-state lighting applications.     

Fluorescence spectroscopic studies on binding of a flavonoid antioxidant quercetin to serum albumins

Binding of quercetin to human serum albumin (HSA) was studied and the binding constant measured by following the red-shifted absorption spectrum of quercetin in the presence of HSA and the quenching of the intrinsic protein fluorescence in the presence of different concentrations of quercetin. Fluorescence lifetime measurements of HSA showed decrease in the average lifetimes indicating binding at a location, near the tryptophan moiety, and the possibility of fluorescence energy transfer between excited tryptophan and quercetin. Critical transfer distance (R _o ) was determined, from which the mean distance between tryptophan-214 in HSA and quercetin was calculated. The above studies were also carried out with bovine serum albumin (BSA).     

Multitargeting by curcumin as revealed by molecular interaction studies

Curcumin (diferuloylmethane), the active ingredient in turmeric (Curcuma longa), is a highly pleiotropic molecule with anti-inflammatory, anti-oxidant, chemopreventive, chemosensitization, and radiosensitization activities. The pleiotropic activities attributed to curcumin come from its complex molecular structure and chemistry, as well as its ability to influence multiple signaling molecules. Curcumin has been shown to bind by multiple forces directly to numerous signaling molecules, such as inflammatory molecules, cell survival proteins, protein kinases, protein reductases, histone acetyltransferase, histone deacetylase, glyoxalase I, xanthine oxidase, proteasome, HIV1 integrase, HIV1 protease, sarco (endo) plasmic reticulum Ca(2+) ATPase, DNA methyltransferases 1, FtsZ protofilaments, carrier proteins, and metal ions. Curcumin can also bind directly to DNA and RNA. Owing to its β-diketone moiety, curcumin undergoes keto-enol tautomerism that has been reported as a favorable state for direct binding. The functional groups on curcumin found suitable for interaction with other macromolecules include the α, β-unsaturated β-diketone moiety, carbonyl and enolic groups of the β-diketone moiety, methoxy and phenolic hydroxyl groups, and the phenyl rings. Various biophysical tools have been used to monitor direct interaction of curcumin with other proteins, including absorption, fluorescence, Fourier transform infrared (FTIR) and circular dichroism (CD) spectroscopy, surface plasmon resonance, competitive ligand binding, Forster type fluorescence resonance energy transfer (FRET), radiolabeling, site-directed mutagenesis, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), immunoprecipitation, phage display biopanning, electron microscopy, 1-anilino-8-naphthalene-sulfonate (ANS) displacement, and co-localization. Molecular docking, the most commonly employed computational tool for calculating binding affinities and predicting binding sites, has also been used to further characterize curcumin's binding sites. Furthermore, the ability of curcumin to bind directly to carrier proteins improves its solubility and bioavailability. In this review, we focus on how curcumin directly targets signaling molecules, as well as the different forces that bind the curcumin-protein complex and how this interaction affects the biological properties of proteins. We will also discuss various analogues of curcumin designed to bind selective targets with increased affinity.     

Structural and optical properties of BaZrO$$_{}$$:Eu$$^{+}$$+phosphor

BaZrO\(_{3}\):Eu\(^{3+}\) perovskite phosphors were successfully synthesized by employing combustion method. The structure, morphology and optical properties of material have been characterized by X-ray diffraction, scanning electron microscopy and fluorescence spectrometry. The XRD results indicate that crystals of BaZrO\(_{3}\):Eu\(^{3+}\) belongs to cubic perovskite system. The phosphors can be effectively excited by UV light and the emission spectra results indicate that reddish-orange luminescence dominates due to parity allowed magnetic dipole transition \(^{5}\)D\(_{0}\rightarrow ^{7}\)F\(_{1}\) located at 593 nm. The prepared phosphor show remarkable luminescent properties which find applications in field emission displays and plasma display panels.     

Properties of phenoxyl radical of dehydrozingerone, a probable antioxidant

Free radical reactions of dehydrozingerone (DZ), a methoxy phenol, were studied at dfferent pHs with a variety of oxidants using nanosecond pulse radiolysis technique. Hydroxyl radical (OH) reaction with the phenolic form at pH 6 led mainly to the formation of an OH-adduct absorbing at 460 nm in addition to a minor oxidation product. On the other hand, at pH 10 with the deprotonated phenoxide ion, the only reaction observable was oxidation generating a phenoxyl radical absorbing at 360 nm. HPLC analysis indicated formation of two different products at pH 6 from addition and oxidation reactions, whereas at pH 10, only the oxidation product was detectable. Reactions of more specific secondary oxidizing radicals, N3√, Br√, Br⁻₂√ and Tl(II) with DZ gave rise to the phenoxyl radical over the entire pH range. DZ in the phenoxide ion form reacted with nitrogen dioxide and trichloromethyl peroxyl radicals with rate constants 6×10⁸ and 8.8×10⁸ dm³ mol⁻¹ s⁻¹ respectively leading to the phenoxyl radicals. The DZ phenoxyl radical reacted with trolox C (an analogue of -tocopherol) with a rate constant of 8.3×10⁷ dm³ mol⁻¹ s⁻¹. One electron reduction potential of the DZ phenoxyl radical at pH 6 was determined to be +1.1 V vs NHE using N3√/N⁻₃ as the standard couple.     

Enantioselective LC analysis and determination of selective serotonin reuptake inhibitors

LC separation of biologically and pharmaceutically important enantiomers (from racemic or non-racemic mixtures) remains a subject of importance. The present review article deals with the liquid chromatographic enantioseparation of chiral selective serotonin reuptake inhibitors (SSRIs), namely citalopram, paroxetine, sertraline and fluoxetine. It is now known that the enantiomers of numerous psychotropic drugs exhibit distinct pharmacodynamics, pharmacokinetic patterns and receptor binding properties, and psychiatric patients are frequently taking more than one medication. Therefore, monitoring of the levels of these analytes in biological fluids is important to determine the levels of enantiomer concentrations; the present paper may be helpful in understanding the present state of available methods (along with a critical discussion of applicability of the methods) and in developing the new ones for this purpose. Different approaches using LC discussed herein may be applied for determining the enantiomeric composition (and enantiomeric purity) of SSRIs and numerous other racemic drugs, of current/future pharmaceutical importance and utility, using simple separation methods, instrumentation, inexpensive reagents and potentially significant analytical approaches. The contents cover the essential data to understand the various separation techniques and associated issues, if any, with documented examples.     

Linear independence of harmonic numbers over the field of algebraic numbers II

The Ramanujan Journal - In a recent article, the authors have studied the arithmetic natures of a certain class of harmonic numbers. The present paper will explore the linear independence of...     

Synthesis,structural analysis,in vitro antioxidant,antimicrobial activity and molecular docking studies of transition metal complexes derived from Schiff base ligands of 4-(benzyloxy)-2-hydroxybenzaldehyde

Research on Chemical Intermediates - Sixteen Co(II), Ni(II), Cu(II) and Zn(II) complexes were synthesized by four Schiff base ligands...