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Cellulolytic enzyme production in aqueous two-phase systems withTrichoderma reesei Rutgers C30 has been investigated. The influ ence of different phase systems, as well as addition of media compo nents and substrate on enzyme production have been studied. Extractive enzyme production in fed-batch cultivations was per formed in a phase system composed of PEG 8000 5%-Dextran T500 7% with 1% Solka-Floc BW 200 as substrate. The cellulolytic enzyme system was intermittently withdrawn with the top phase. Addition of media components every 24 h and cellulose every 72 h gave an aver age enzyme activity in the withdrawn top phase of 2.2 FPU/mL dur ing 170 h cultivation. The corresponding productivity was 18 FPU/lh. The productivity was increased to 24 FPU/l.h when media compo nents and cellulose were added every 72 h. The average enzyme con centration was then 1.6 FPU/mL. The results are discussed in relation to methods for cellulolytic enzyme production involving immobiliza tion and cell recycling.  相似文献   
44.
This paper reports new results on the optical spectra of Na particles and on laser-induced photodissociation of Na atoms from the surface of these particles. In continuation of our earlier studies we have performed experiments to elucidate the mechanism of thenonthermal dissociation process. Furthermore, theoretical calculations have been carried out with the goal to correlate the wavelength dependence of the photodissociation yield with the optical absorption spectra of the metal particles. In addition, manipulation of the size distribution of metal particles on supports is outlined as an application of the effect. This allows for the preparation of very special surfaces with novel physical and chemical properties.  相似文献   
45.
We prove that the nodal set (zero set) of a solution of a generalized Dirac equation on a Riemannian manifold has codimension 2 at least. If the underlying manifold is a surface, then the nodal set is discrete. We obtain a quick proof of the fact that the nodal set of an eigenfunction for the Laplace-Beltrami operator on a Riemannian manifold consists of a smooth hypersurface and a singular set of lower dimension. We also see that the nodal set of a Δ-harmonic differential form on a closed manifold has codimension 2 at least; a fact which is not true if the manifold is not closed. Examples show that all bounds are optimal. Received: 28 October 1996 / Accepted: 3 March 1997  相似文献   
46.
We study game formulas the truth of which is determined by a semantical game of uncountable length. The main theme is the study of principles stating reflection of these formulas in various admissible sets. This investigation leads to two weak forms of strict-II11 reflection (or ∑1-compactness). We show that admissible sets such as H2) and Lω2 which fail to have strict-II11 reflection, may or may not, depending on set-theoretic hypotheses satisfy one or both of these weaker forms. Mathematics Subject Classification : 03C70, 03C75.  相似文献   
47.
Brandau  C.  Bartsch  T.  Böhm  S.  Böhme  C.  Hoffknecht  A.  Kieslich  S.  Knopp  H.  Schippers  S.  Shi  W.  Müller  A.  Grün  N.  Scheid  W.  Steih  T.  Bosch  F.  Franzke  B.  Kozhuharov  C.  Krämer  A.  Mokler  P. H.  Nolden  F.  Steck  M.  Stöhlker  T.  Stachura  Z. 《Hyperfine Interactions》2003,146(1-4):41-45
Hyperfine Interactions - An overview of measurements of dielectronic recombination (DR) with the heaviest lithiumlike ions is presented. The experiments have been carried out at the heavy ion...  相似文献   
48.
In this paper, the development of decision support tools for dynamic ambulance relocation and automatic ambulance dispatching is described. The ambulance dispatch problem is to choose which ambulance to send to a patient. The dynamic ambulance relocation problem occurs in the operational control of ambulances. The objective is to find new locations for some of the ambulances, to increase the preparedness in the area of responsibility. Preparedness is a way of evaluating the ability to serve potential patients with ambulances now and in the future. Computational tests using a simulation model show that the tools are beneficial in reducing the waiting periods for the patients.  相似文献   
49.
High-accuracy film thickness measurements in the range below 100 nm can be made by various complex methods like spectral ellipsometry (SE), scanning force microscopy (SFM), grazing incidence X-ray reflectometry (GIXR), or X-ray fluorescence analysis (XRF). The measurement results achieved with these methods are based on different interactions between the film and the probe. A key question in nanotechnology is how to achieve consistent results on a level of uncertainty below one nanometre with different techniques.Two different types of thickness standards are realised. Metal film standards for X-ray techniques in the thickness range 10 to 50 nm are calibrated by GIXR with monochromatised synchrotron radiation of 8048 eV. The results obtained at four different facilities show excellent agreement. SiO2 on Si standards for SE and SFM in the thickness range 6 to 1000 nm are calibrated by GIXR with monochromatised synchrotron radiation of 1841 eV and with a metrological SFM. Consistent results within the combined uncertainties are obtained with the two methods. Surfaces and interfaces of both types of standards are additionally investigated by transmission electron microscopy (TEM). PACS 61.10.Kw; 68.55.Jk; 06.20.Fn; 06.60.Mr; 07.79.Lh  相似文献   
50.
We review many-body calculations of the equation of state of dilute neutron matter in the context of effective-field theories of the nucleon-nucleon interaction.  相似文献   
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