Hybridization of binary monolayers of single stranded oligonucleotides and short blocking molecules

We have studied the immobilization of single stranded (ss) DNA oligonucleotides of 16-27 base pairs on gold. The oligonucleotides were thiol-modified (SH-ssDNA) or disulfide-modified via a dimethoxytrityl-group (DMT-S-S-ssDNA). Immobilization was performed by adsorption of the probes on the gold surface for 10-15 min, a time within which saturation coverage was obtained for both thiol- and disulfide-modified probes. Hereafter the layer was post-treated with hydroxyalkyl substituted lipoamides also for a time of 10-15 min. The surface density of layers with shorter probes (16-18 mer) was twice (2.4 ± 0.2 × 10¹³ probes/cm²) that of the longer probes (25-27 mer) as studied with surface plasmon resonance. Hybridization of single stranded polymerase chain reaction (PCR) amplified products with a length above 300 base pairs gave a very low hybridization response. For amplicons with about 100 base pairs the response was high. The surface coverage was comparable to that of complementary ssDNA binding (3.0 × 10¹² strands/cm²). Surfaces made from SH-ssDNA showed a 30% higher hybridization response than surfaces made from DMT-S-S-ssDNA. The PCR amplified products used are of relevance in breast cancer diagnosis. The results clearly demonstrate that the single stranded PCR products might be used in label-free cancer diagnostics.     

Ab initio description of high-temperature superconductivity in dense molecular hydrogen

We present a first-principles study of the electron-phonon interaction and the prediction of the superconducting critical temperature in molecular metallic hydrogen. Our study is able to single out the features which drive the system towards superconductivity: mainly, a rich and complex Fermi surface and strongly coupled phonon modes driving the intra- or intermolecular charge transfer. We demonstrate that in this simple system, a very high superconducting critical temperature can be reached via electron-phonon and Coulomb electron-electron interactions.     

Spectral shaping of femtosecond pulses in aperiodic quasi-phase-matched gratings

We analyze the use of cascading second harmonic interactions in quadratic nonlinear crystals to mould the spectral characteristics of broadband near-infrared femtosecond pulses. Using a genetic algorithm, we optimize the design of the aperiodically poled ferroelectric crystal capable of generating the desired femtosecond infrared pulsed radiation.     

Design and synthesis of novel polycycles based on the 3H‐pyrrolo/6,7‐dihydropyrido[1,2‐a]indole scaffold as templates for pharmaceutical development

In the course of our work aimed at developing novel heterocycles of pharmaceutical interest, we designed and synthesized several polycyclic templates as potential substrates to be used in drug design. We obtained a set of condensed ring systems as versatile structural platforms to generate potential DNA‐interactive agents and/or reversible inhibitors of enzymes such as topoisomerases, poly(ADP‐ribose) polymerase‐1 (PARP‐1), telomerase, and, in particular, cyclin dependent kinases. Herein, we report the design, synthesis, structural investigation, and preliminary DNA‐binding affinity of these heteroaromatic systems. J. Heterocyclic Chem., (2011).     

Students’ Activity in Computer-Supported Collaborative Problem Solving in Mathematics

The purpose of this study was to analyse secondary school students’ (N = 16) computer-supported collaborative mathematical problem solving. The problem addressed in the study was: What kinds of metacognitive processes appear during computer-supported collaborative learning in mathematics? Another aim of the study was to consider the applicability of networked learning in mathematics. The network-based learning environment Knowledge Forum (KF) was used to support students’ collaborative problem solving. The data consist of 188 posted computer notes, portfolio material such as notebooks, and observations. The computer notes were analysed through three stages of qualitative content analysis. The three stages were content analysis of computer notesin mathematical problem solving, content analysis of mathematical problem solving activity and content analysis of the students’ metacognitive activity. The results of the content analysis illustrate how networked discussions mediated mathematical knowledge and students’ questions, while the mathematical problem solving activity shows that the students co-regulate their thinking. The results of the content analysis of the students’ metacognitive activity revealed that the students use metacognitive knowledge and make metacognitive judgments and perform monitoring during networked discussions. In conclusion, the results of this study demonstrate that working with the networked technology contributes to the students’ use of their mathematical knowledge and stimulates them into making their thinking visible. The findings also show some metacognitive activity in the students’ computer-supported collaborative problem solving in mathematics.     

On Codes Identifying Sets of Vertices in Hamming Spaces

A code is called (t, 2)-identifying if for all the words x, y(x y) and the sets (B _t (x) B _t (y)) C and are nonempty and different. Constructions of such codes and a lower bound on the cardinality of these codes are given. The lower bound is shown to be sharp in some cases. We also discuss a more general notion of -identifying codes and introduce weakly identifying codes.     

Time-harmonic solution for acousto-elastic interaction with controllability and spectral elements

The classical way of solving the time-harmonic linear acousto-elastic wave problem is to discretize the equations with finite elements or finite differences. This approach leads to large-scale indefinite complex-valued linear systems. For these kinds of systems, it is difficult to construct efficient iterative solution methods. That is why we use an alternative approach and solve the time-harmonic problem by controlling the solution of the corresponding time dependent wave equation.In this paper, we use an unsymmetric formulation, where fluid-structure interaction is modeled as a coupling between pressure and displacement. The coupled problem is discretized in space domain with spectral elements and in time domain with central finite differences. After discretization, exact controllability problem is reformulated as a least-squares problem, which is solved by the conjugate gradient method.     

A control method for speeding up response of hydroelectric station power canals

A control method which, through the suitable servo-operation of the check gates, attains a consistent improvement in the speed of response of hydroelectric power canals is presented. Its application allows the size of the canal headpond to be reduced at the design stage of the power station as well. The proposed method is based on the constant volume control concept and uses a mathematical model of the power canal derived from linearization of the Saint-Venant equations. The results of the computer simulation of a controlled real system's dynamic behavior obtained through the use of the method of characteristics are presented.     

Sub-optimal constant-volume control for open channel networks

A state variable mathematical model for use in the synthesis of automatic control systems for open-channel networks is presented. The system considered here consists of n-cascaded reaches joined by control gates.The linear time invariant model consists of a controllable and observable representation where the state variables are the stored water volume variations in each reach and the control signals are the variations of the control gates opening sections. The model derives, through appropriate simplifications, from a more complex one in terms of transfer functions which was derived by linearizing the Saint-Venant equations.The problem of a linear quadratic optimal regulator is formulated in classical terms for the canal system and the constant-volume control laws obtained for the simplified model have been imposed on the complex one: such a control is therefore to be considered sub-optimal.The results of a digital simulation of the controlled system behaviour indicate that the system operates with practically constant stored water volumes in each reach and that such behaviour is fairly close to that of a pressure-water pipe system.     

Low-energy electron scattering by cubane: resonant states and Ramsauer-Townsend features from quantum calculations in the gas phase

Calculations are carried out, using a nonempirical modeling of the interaction potential and solving the quantum scattering coupled channel equations, for low energy electron scattering from cubane (C8H8) molecules in the gas phase. Total integral cross sections are obtained and partial contributions are analyzed for the most important irreducible representations that describe the continuum electron in the Oh molecular symmetry. Several trapping resonances are found and analyzed in terms of the molecular-type features of the resonant electron states associated with them. A Ramsauer-Townsend minimum is also found and its possible behavior related to features of the scattering length as k --> 0.