Surface Functionalized Magnetic Nanoparticles as a Selective Sorbent for Affinity Fishing of PPAR-γ Ligands from Choerospondias axillaris

Coronary heart disease (CHD), which has developed into one of the major diseases, was reported to be treated by the target of peroxisome proliferators-activate receptor γ (PPAR-γ). As a natural medicine long used in the treatment of CHD, there are few studies on how to screen the target active compounds with high specific activity from Choerospondias axillaris. To advance the pace of research on target-specific active compounds in natural medicines, we have combined magnetic ligand fishing and functionalized nano-microspheres to investigate the active ingredients of PPAR-γ targets in Choerospondias axillaris. The PPAR-γ functionalized magnetic nano-microspheres have been successfully synthesized and characterized by vibrating sample magnetometer (VSM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The specificity, reusability, and reproducibility of the nano-microspheres were investigated with the help of the specific binding of rosiglitazone to PPAR-γ. In addition, the incubation temperature and the pH of the buffer solution in the magnetic ligand fishing were optimized to improve the specific adsorption efficiency of the analytes. Finally, with the aid of ultraperformance liquid chromatography plus Q-Exactive Orbitrap tandem mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS/MS), the 16 active ligands including 9 organic acids, 5 flavonoids, and 2 phenols were found in the ethanolic extracts of Choerospondias axillaris. Therefore, the study can provide a successful precedent for realizing the designated extraction and rapid isolation of target-specific active ingredient groups in the complex mixtures.     

相位图法研究光子晶体的禁带

给出了一种判断一维光子晶体禁带位置的相位图,利用扩展相位图可方便地描述光子晶体的禁带位置和禁带特征.研究发现,当光子晶体为1/4波片层堆时,光子晶体的禁带最宽;若要进一步展宽禁带,需提高构成周期单元的两种介质的折射率比.对于一般的光子晶体,若周期单元中两种介质的光学厚度不等,则其禁带中心将偏离中心频率的整数倍.此外还研究了禁带中心区的透射率,给出了中心频率附近透射率的一级近似解析解,并由此定性讨论了Fabry-Perot腔的谱线宽度和品质因子.     

飞行器上层大气层空气动力特性探讨

针对近地轨道飞行器所面临的上层大气层(100～300 km)空气动力学问题,对几类典型航天器构型的上层大气层气动力特性进行了分析,给出了典型气动布局在该空域的气动力基本规律,取得了对上层大气层气动力关键影响因素的初步认识.在上层大气层,飞行器的绕流属于自由分子流状态.研究发现,气体分子与不同材质物面的相互作用反映出截然...     

对数比放大电路在精密光功率计中的应用

光功率测量是光纤通信系统最基本的测量参数之一.在实际测量中,光功率计要求有很大的动态测量范围.采用传统线性放大电路设计的光功率计,存在着换档误差,动态测量范围也受到限制.由于结果显示要进行对数运算,软件处理也相对比较复杂.为改善上述不足,使用对数比放大电路代替传统的线性放大电路.这样可以避免换档误差和繁琐的对数运算,提高精度.经过实际设计验证,改进设计可获得80 dB动态范围,0.1 dLBm的测量精度,而且电路简洁、易于校准.此设计方法已在实际产品得以应用.     

铜电路板缝腐蚀过程缝隙中pH、Cl-浓度分布的测量

根据铜电路板缝腐蚀特征,研制了阵列式Ag/AgCl、IrO2电极,设计缝隙腐蚀模拟装置,在0.5mol.L-1的NaCl溶液中分别同时原位检测电子线路板缝隙腐蚀过程,缝隙内的氯离子浓度分布、pH分布及其随时间的变化.研究表明,在电子线路板发生缝隙腐蚀的过程中,缝隙内部不同深度的Cl-及H+浓度逐渐增大,且随着与缝口距离的增大而增大,从而导致缝隙腐蚀不断向纵深方向发展.     

用COMSOL实现圆波导中电磁波的可视化

为了直观描述金属圆波导中电磁场复杂的空间分布,本文用Comsol软件对金属圆波导中的TE11模、TE01模、和TM01模的电磁场结构进行动态仿真.将电磁场中的抽象概念形象化、可视化,使学生看清电磁场分布的宏观概况和细节变化,开拓了视野,同时加深了学生对电磁波传播特性的理解.     

微电子专业的通才和专才培养模式

国内微电子产业迅猛发展,支持在低端产品的基础技术人员的储备已经达到相当规模,但自主研发高端产品的专业人才匮乏。目前高等学校微电子专业改革中,在通才和专才培养模式之间很难找到平衡点。本文通过对比中美的微电子人才培养模式,讨论微电子专业通才和专才的培养模式。     

模电教学中工程观念的培养

"模拟电子线路"课程是工科本科电子信息类专业的一门重要的专业基础课程.本文以集成运放电路在压力检测中的应用为例,探讨了模拟电子线路课程教学中如何将理论联系实际、如何培养工程观念、如何将基本理论与工程应用相结合等问题.通过课程教案示例,阐述了工程观念的培养在教学实践中的具体做法.     

一种实时数字视频内容识别方法

提出了一种在数字电视和流媒体播出过程中进行实时视频内容识别的算法模型,讨论了该模型的几个性质,同时给出一种融合不同视觉特征模型的视频匹配方法,并针对数字电视TS流进行了算法测试,得到了准确实时的内容检测效果.     

Simultaneous Determination of Six Immunosuppressants in Human Whole Blood by HPLC-MS/MS Using a Modified QuEChERS Method

A high-performance liquid chromatography-tandem mass spectrometry method was established for the simultaneous determination of mycophenolic acid, mycophenolate mofetil, tacrolimus, rapamycin, everolimus and pimecrolimus in human whole blood by optimizing the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) preparation method. Whole blood was extracted into ethyl acetate, salted out with anhydrous magnesium sulfate, and purified with ethylenediamine-N-propyl silane adsorbent. The supernatant was evaporated under nitrogen until dry and finally reconstituted in methanol. Chromatographic separation was performed on an Agilent Poroshell 120 EC-C18 column in methanol (mobile phase A)-water (optimized for 0.1% acetic acid and 10 mM ammonium acetate, mobile phase B) at a 0.3 mL·min⁻¹ flow rate. Electrospray ionization and positive ion multiple reaction monitoring were used for detection. The time for of analysis was 13 min. The calibration curves range of tacrolimus, rapamycin, everolimus and pimecrolimus were in the range of 1–100 ng·mL⁻¹, mycophenolate mofetil in the range of 0.1–10 ng·mL⁻¹ and mycophenolic acid at 10–1000 ng·mL⁻¹. All correlation coefficients were >0.993. The coefficients of variation (CV, %) for inter-day and intra-day precision were less than 10%, while the spiked recoveries were in the range of 92.1% to 116%. Our method was rapid, sensitive, specific, and reproducible for the simultaneous determination of six immunosuppressants in human whole blood. Importantly, our approach can be used to monitor drug concentrations in the blood to facilitate disease treatment.