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101.
MRI and x-ray CT study of spatial distribution of core breakdown in 'Conference' pears 总被引:7,自引:0,他引:7
Lammertyn J Dresselaers T Van Hecke P Jancsók P Wevers M Nicolaï BM 《Magnetic resonance imaging》2003,21(7):805-815
Two non-destructive tomographic techniques, X-ray CT imaging and magnetic resonance imaging (MRI), were applied to study the development of core breakdown disorder in 'Conference' pears (Pyrus communis cv. Conference). This disorder, which is characterized by brown discoloration of the tissue and development of cavities, is induced by elevated CO(2) and decreased O(2) levels during controlled atmosphere storage. Tomographic images of pears stored for 10 months under disorder inducing conditions, were acquired with both techniques and compared to the actual slices. Both X-ray and MRI were able to differentiate between unaffected tissue, brown tissue and cavities. A simple image-processing program, based on threshold values, was developed to determine the area percentage of affected and unaffected tissue as well as the cavity and core area per slice. For all three imaging techniques the area percentage brown tissue per slice increased with the diameter of the pear, but was systematically underestimated by 12% and 6% for, respectively, X-ray and MRI, compared to the actual slices. The area percentage cavity corresponded very well for all techniques. It was also found that the contours of the brown tissue were parallel to the fruit boundaries, suggesting a relation between the disorder symptoms and gas diffusion properties of the fruit. It was concluded that MRI is the most appropriate technique to study the development of core breakdown disorder during postharvest storage in future experiments. 相似文献
102.
Giampiero Mei Almerinda Di Venere Eleonora Nicolai Nicola Rosato Alessandro Finazzi Agro' 《Journal of fluorescence》2003,13(1):33-39
Tryptophan fluorescence is extremely useful to monitor structural conformational transitions in proteins. Denaturant-induced unfolding of azurin and ascorbate oxidase has been studied by dynamic fluorescence measurements in the frequency domain and the results have been interpreted in terms of continuous distribution of lifetimes. The data add new information on the unfolding mechanism that was previously analyzed by steady-state emission spectroscopy. In particular, the existence of multiple, parallel unfolding pathways may be envisaged and correlated, in both cases, to the two protein structures. The effect of metal depletion has been also characterized by fluorescence lifetime measurements. In the case of azurin, a monomeric protein, the data demonstrate that copper removal yields a totally different unfolding pathways with respect to the holo protein, indicating that metal ion plays a fundamental structural role in the wild type, native protein. In the case of ascorbate oxidase a dimer of 140 kDa, only minor effects have been detected by copper removal. However, the analysis of the fluorescence decay in presence of different amounts of guanidinium hydrochloride gives new important insights on the unfolding intermediates. In particular the data support the hypothesis of a partial exposure of an outer layer of dimer at intermediate denaturant concentration. This ability of dynamic fluorescence to pinpoint the presence of structural micro-heterogeneity in the unfolding pathways of proteins demonstrates the greater power of this technique compared to the most commonly used steady-state measurements. 相似文献
103.
Gunaratne T Rodgers MA Felder D Nierengarten JF Accorsi G Armaroli N 《Chemical communications (Cambridge, England)》2003,(24):3010-3011
Transient absorption spectrometry of Cu(I)-phenanthrolines in CH2Cl2 reveals ligand-independent dynamic processes lasting 15 ps, which are associated with the peculiar structural rearrangements occurring for this class of compounds upon photoexcitation. 相似文献
104.
De Nicola A Liu Y Schanze KS Ziessel R 《Chemical communications (Cambridge, England)》2003,(2):288-289
A facile and original synthesis of four 2,2'-bipyridine (bipy) ligands grafted with thiophene subunits is described using phase transfer experimental conditions: related Ru(II) complexes exhibit well-defined redox and photophysical properties which were probed by cyclic voltammetry, UV-vis, steady-state emission and transient absorption spectroscopy. 相似文献
105.
Starch and its analogue glycogen are biosynthesized by enzymes that have been classified by sequence similarities into two families that have no significant sequence overlap: the animal/fungal glycogen synthases and the plant/bacterial glycogen (starch) synthases. Recent gene sequence analysis of putative archaea enzymes implicates them as a third family that links the structural and functional features of the other two classes. Herein, we present the first rapid electrospray ionization mass spectrometry-based assay to quantify any carbohydrate-polymerizing activity, the first cloning and recombinant expression as well as verification of the putative function of a glycogen synthase from the hyperthermophilic archaea Pyrococcus furiosus, and the characterization of a variety of glycogen synthases with the new assay. The new assay allowed the determination of Km and Vmax values for the rabbit, yeast, and P. furiosus glycogen synthases. Most surprisingly, unlike the synthases from rabbit or yeast and in contradiction to what would be expected from structural studies of other nucleotide-sugar binding proteins, the synthase from the archaea source accepts both uridine- and adenine-diphosphate activated glucose competitively and with comparable affinities to form a glucose polymer. This loose substrate specificity implicates this protein as the chemical link between the two branches of glycogen synthases that have evolved to accept primarily one or the other nucleotide as well as a good source enzyme for polymer bioengineering efforts. 相似文献
106.
[reaction: see text] Addition of isopropyl-beta-d-thiogalactopyranoside (IPTG) to bacterial cultures is often used to induce expression of plasmid-based genes for the production of recombinant proteins under control of the lac promoter, but a simple method to circumvent the inherent instability of this compound has not been addressed experimentally. Herein we report the first synthesis of isobutyl-C-galactoside (IBCG), the C-glycoside analogue of IPTG, and show that IBCG is superior to IPTG in inducing protein expression over long induction times. 相似文献
107.
Nicola Garofalo Elena Sartori 《Proceedings of the American Mathematical Society》2001,129(12):3603-3610
We use the method of Alexandroff-Serrin to establish the spherical symmetry of the ground domain and of the weak solution to a free boundary problem for a class of quasi-linear parabolic equations in an unbounded cylinder , where , with a simply connected bounded domain. The equations considered are of the type , with modeled on . We consider a solution satisfying the boundary conditions: for , and , as . We show that the overdetermined co-normal condition for , with 0$"> for at least one value , forces the spherical symmetry of the ground domain and of the solution.
108.
Liliana Caristia Giuseppe Nicotra Corrado Bongiorno Nicola Costa Sebastiano Ravesi Salvo Coffa Riccardo De Bastiani Maria Grazia Grimaldi Corrado Spinella 《Microelectronics Reliability》2007,47(4-5):777
This work reports the study concerning the influence of the preparation conditions on the structure of silicon rich oxide (SRO) deposited by PECVD method by which the structural properties of the film are strictly related. In particular we investigated the role of reactant gases N2O and SiH4 on the total Si concentration, Si excess concentration, Si clustered concentration and size of nanoclusters formed by high annealing temperature. We payed particular attention on the role of the hydrogen and nitrogen during the Si agglomeration.The presence of hydrogen atoms on the as-deposited specimen, confirmed by the Si–H bonds peak on the FTIR analysis, has been directly correlated to the silicon excess concentration in the layer. The silicon, oxygen and nitrogen atomic density has been calculated from RBS analysis. These information were coupled to the ones obtained using methodology based on electron energy loss spectroscopy combined with energy filtered images, which allowed us to quantify the clustered silicon concentration in annealed sub-stoichiometric silicon oxide layers (SiOx). We have verified that the nitrogen dissolved in the layer inhibits the Si excess clustering so that the efficiency of silicon agglomeration process decreases as the nitrogen content increases. 相似文献
109.
Beniamino Cappelletti Montano Antonio De Nicola 《Journal of Geometry and Physics》2007,57(12):2509-2520
We present a compared analysis of some properties of 3-Sasakian and 3-cosymplectic manifolds. We construct a canonical connection on an almost 3-contact metric manifold which generalises the Tanaka–Webster connection of a contact metric manifold and we use this connection to show that a 3-Sasakian manifold does not admit any Darboux-like coordinate system. Moreover, we prove that any 3-cosymplectic manifold is Ricci-flat and admits a Darboux coordinate system if and only if it is flat. 相似文献
110.
Hetherington NB Kulak AN Sheard K Meldrum FC 《Langmuir : the ACS journal of surfaces and colloids》2006,22(5):1955-1958
Single crystals of calcite with regular patterned surfaces comprising close-packed arrays of hemispherical cavities or domes were produced by crystallization on colloidal monolayers or PDMS replicas of these monolayers, respectively. Perfect replication of the substrate topography was achieved for all colloidal particles, irrespective of their size and surface chemistry when the substrate geometry permitted unrestricted ion flow to the growing crystal. This work demonstrates that crystallization within a mould provides a very general route to producing single crystals with curved surfaces and unusual morphologies and that such patterning can be applied from the micro- to the nanoscale. 相似文献