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71.
The most popular in vitro nucleic acid amplification techniques like polymerase chain reaction (PCR) including real-time PCR are costly and require thermocycling, rendering them unsuitable for uses at point-of-care. Highly efficient in vitro nucleic acid amplification techniques using simple, portable and low-cost instruments are crucial in disease diagnosis, mutation detection and biodefense. Toward this goal, isothermal amplification techniques that represent a group of attractive in vitro nucleic acid amplification techniques for bioanalysis have been developed. Unlike PCR where polymerases are easily deactivated by thermally labile constituents in a sample, some of the isothermal nucleic acid amplification techniques, such as helicase-dependent amplification and nucleic acid sequence-based amplification, enable the detection of bioanalytes with much simplified protocols and with minimal sample preparations since the entire amplification processes are performed isothermally. This review focuses on the isothermal nucleic acid amplification techniques and their applications in bioanalytical chemistry. Starting off from their amplification mechanisms and significant properties, the adoption of isothermal amplification techniques in bioanalytical chemistry and their future perspectives are discussed. Representative examples illustrating the performance and advantages of each isothermal amplification technique are discussed along with some discussion on the advantages and disadvantages of each technique. 相似文献
72.
用于脉冲多普勒雷达的高速数字自动增益控制(DAGC)系统 总被引:4,自引:1,他引:4
本文从AGC的本质入手,避开复杂的数学计算,提出了一种新的地址指针查表式的DAGC系统,介绍了该系统的工作原理及工作流程。 相似文献
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Jumi Yun Ji Sun Im Young-Seak Lee Hyung-Il Kim Author vitae 《European Polymer Journal》2011,47(10):1893-1902
The electro-responsive transdermal drug delivery system was prepared by electrospinning of poly(vinyl alcohol)/poly(acrylic acid)/multi-walled carbon nanotubes (MWCNTs) nanocomposites. The surface modification of MWCNTs was carried out by oxyfluorination to introduce the functional groups on the hydrophobic MWCNTs. The dispersion of MWCNTs and the compatibility with polymer matrices were improved by oxyfluorination. The MWCNT content and oxyfluorination condition played important roles in the swelling and drug release characteristics of nanofibers. The conductivity of nanofibers increased by increasing the content of MWCNTs and performing oxyfluorination with higher oxygen content. Uniform distribution of the oxyfluorinated MWCNTs in the nanofibers was crucial to the electro-responsive swelling and drug releasing behaviors of nanofibers. 相似文献
76.
High pesticide use, especially in agriculture, can lead to environmental pollution and potentially adverse health effects. As result, pesticide residues end up in different media, including water and food products, which may serve as direct routes for human exposure. There is thus a continuous drive to develop analytical methods for screening and quantification of these compounds in the different environmental media in which they may occur. Development of quantum dot (QD) based sensors for monitoring pesticides has gained momentum in recent years. QD materials have excellent and unique optical properties and have high fluorescence quantum yields compared to other fluorophores. They have thus been used in numerous studies for the development of probes for organic pollutants. In this paper we specifically review their application as fluorescence probes for pesticide detection in different media including water and in fruits and vegetables. The low detection limits reported demonstrate the potential use of these methods as alternatives to expensive and time-consuming conventional techniques. We also highlight potential limitations that these probes may present when it comes to routine application. Finally we discuss possible future improvements to enhance the selectivity and robustness of these sensors. We note that there is still a need for researchers to develop standardized QD based sensors which could lead to their commercialization and routine application. 相似文献
77.
Congyi Liu Author VitaeChunxiao ChiganAuthor Vitae 《Ad hoc Networks》2012,10(3):497-511
To improve traffic safety and efficiency, it is vital to reliably send traffic-related messages to vehicles in the targeted region in vehicular ad hoc networks (VANETs). In this paper, we propose a novel scheme, relative position based message dissemination (RPB-MD), to reliably and efficiently disseminate messages to the vehicles in the zone-of-relevance. Firstly, the relative position based (RPB) addressing model is proposed to effectively define the intended receivers in the zone-of-relevance. To ensure high message delivery ratio and low delivery delay, directional greedy broadcast routing (DGBR) is introduced to make a group of candidate nodes hold the message for high reliability. Moreover, to guarantee efficiency, the protocol time parameters are designed adaptively according to the message attributes and local vehicular traffic density. The protocol feasibility is analyzed to illustrate the robustness and reliability of RPB-MD. Simulation results show that RPB-MD, compared with representative existing schemes, achieves high delivery ratio, limited overhead, reasonable delay and high network reachability under different vehicular traffic density and data sending rate. 相似文献
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合成了 7种草酸根桥联的 Cu 2 Fe 、Ni 2 Fe 、Co 2 Fe 异三核配合物 [M2 Fe(C2 O4) 3Lx](Cl O4) ,(M=Cu,L=bpy,Me2 phen,NO2 phen,x=2 ;M=Ni,Co,L=bpy,Me2 phen,x=4 ) .经元素分析、摩尔电导和磁性的测定以及红外光谱和电子光谱等方法对这些配合物进行了表征 ,确定了配合物的组成和结构 .初步生物活性试验表明形成异三核配合物后其杀菌活性明显提高 相似文献
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M. Meysam Zargham Author Vitae Christian Schlegel Author Vitae 《Integration, the VLSI Journal》2010,43(4):365-377
Analog implementations of digital error control decoders, generally referred to as analog decoding, have recently been proposed as an energy and area competitive methodology. Despite several successful implementations of small analog error control decoders, little is currently known about how this methodology scales to smaller process technologies and copes with the non-idealities of nano-scale transistor sizing. A comprehensive analysis of the potential of sub-threshold analog decoding is examined in this paper. It is shown that mismatch effects dominated by threshold mismatch impose firm lower limits on the sizes of transistors. The effect of various forms of leakage currents is also investigated and minimal leakage current to normalizing currents are found using density evolution and control simulations. Finally, the convergence speed of analog decoders is examined via a density evolution approach. The results are compiled and predictions are given which show that process scaling below 90 nm processes brings no advantages, and, in some cases, may even degrade performance or increase required resources. 相似文献