A High Sensitive,Reproducible and Disposable Immunosensor for Analysis of SOX2

In this study, an ITO (indium tin oxide) based biosensor was constructed to detect SOX2. SOX2 helps the regulation of cell pluripotency and is closely related to early embryonic development, neural and sexual differentiation. SOX2 is amplified and overexpressed in some malignant tumors such as squamous cell, lung, prostate, breast, esophageal cell, colon, ovarian, glioblastoma, pancreatic cancer, gastric cancer, head and neck squamous cell carcinoma. To generate a hydroxylated clean electrode surface, ITO electrodes were treated with NH₄OH/H₂O₂/H₂O. Later, ITO‐PET electrode surfaces were modified with 3‐glycidoxypropyl trimethoxysilane (3‐GOPS). Then, Anti‐SOX2 was covalently immobilized onto the electrode surfaces. 3‐GOPS concentration, Anti‐SOX2 concentration and incubation time, SOX2 incubation time were optimized. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were utilized in order to follow up the immobilization processes and the optimization steps of the biosensor. To characterize the analytical properties of constructed immunosensor; linear range, repeatability, reproducibility and regeneration studies were investigated. The linear range of the immunosensor was detected as 0.625 pg/mL–62.5 pg/mL. Square wave voltammetry technique was also applied to the biosensor. Storage life of the biosensor was determined for identifying the possible usability of the biosensor in clinical field. Finally, the designed biosensor was applied to the real human serum samples. The results obtained with the presented biosensor were also compared with ELISA results.     

Electrical Conductivity in a Laser Beam Channel

Russian Physics Journal - Electrical conductivity in a laser beam channel has been investigated. Oscillations of the conductivity caused by the oscillation motion of electrons in laser-induced...     

Energy Characteristics of Non-Resonator Generation in Nanodispersed Active Media Irradiated with Femtosecond Laser Pulses

Russian Physics Journal - Results of experimental measurements of the energy characteristics of stimulated emission in coumarin-30 and rhodamine 6G dyes with nanoparticles under irradiation with...     

ESR determination of the thermal stability of Mn<Superscript>2+</Superscript> ion binding sites in different legume lectins

The legume lectins are a large family of homologous carbohydrate-binding proteins. Their carbohydrate specificity and quaternary structure vary widely. The carbohydrate binding activity of legume lectins depends on the simultaneous presence of calcium and transition-metal ions, especially the Mn²⁺ ion. In the present work, thermal stabilities of Mn²⁺ binding sites in pea, lentil, soybean and kidney-bean lectins have been studied by electron spin resonance spectroscopy in the temperature range of 120–400 K in different atmospheres. The evolution of parameters, such as the line width, peak-to-peak intensity associated with the hyperfine transition [m _I (+5/2) → m′ _I (−5/2)] at the lowest magnetic field side of the central electron transition [m _S (+1/2) → m′ _S (−1/2)] of the Mn⁺² ion, g-factor and hyperfine splitting was evaluated. Annealing was also performed at high temperatures (353, 373, 383, 403 and 443 K) and the oxidation activation energy of Mn²⁺ ions in the indicated legumes was determined.     

Nanoplasmonic directional couplers and Mach–Zehnder interferometers

We present a novel design and analysis of two nano-scale plasmonic devices: a directional coupler and a Mach–Zehnder interferometer. The designs of the two devices are based on our recent work on the air-gap coupler that resulted in high coupling efficiency between a dielectric waveguide and a plasmonic waveguide. The two devices are embedded between two dielectric waveguides and operate at optical telecom wavelengths. The overall efficiency was 37% for a 2×2 directional coupler switch and above 50% for the proposed designs for a Mach–Zehnder Interferometer. The efficiency in the proposed devices can be increased using broader plasmonic waveguides.     

Joint bounds for the Perron roots of nonnegative matrices with applications

Given a finite set {A_x}_{x ∈ X} of nonnegative matrices, we derive joint upper and lower bounds for the row sums of the matrices D⁻¹ A^(x) D, x ∈ X, where D is a specially chosen nonsingular diagonal matrix. These bounds, depending only on the sparsity patterns of the matrices A^(x) and their row sums, are used to obtain joint two-sided bounds for the Perron roots of given nonnegative matrices, joint upper bounds for the spectral radii of given complex matrices, bounds for the joint and lower spectral radii of a matrix set, and conditions sufficient for all convex combinations of given matrices to be Schur stable. Bibliography: 20 titles. __________ Translated from Zapiski Nauchnykh Seminarov POMI, Vol. 334, 2006, pp. 30–56.     

Synthesis,Characterization, DNA Binding Studies,Photocleavage, Cytotoxicity and Docking Studies of Ruthenium(II) Light Switch Complexes

A new ligand 3-(1H-imidazo[4,5-f][1,10]phenanthrolin-2yl)phenylboronic acid and its (IPPBA) three ruthenium(II) complexes [Ru(phen)₂(IPPBA)](ClO₄)₂ (1), [Ru(bpy)₂(IPPBA)](ClO₄)₂ (2) and [Ru(dmb)₂(IPPBA)](ClO₄)₂ (3) have been synthesized and characterized by elemental analysis, UV/VIS, IR, ¹H-NMR,¹³C-NMR and mass spectra. The binding behaviors of the three complexes to calf thymus DNA were investigated by absorption spectra, emission spectroscopy, viscosity measurements, thermal denaturation and photoactivated cleavage. The DNA-binding constants for complexes 1, 2 and 3 have been determined to be 7.9?×?10⁵ M^?1, 6.7?×?10⁵ M^?1 and 2.9?×?10⁵ M^?1. The results suggest that these complexes bound to double-stranded DNA in an intercalation mode. Upon irradiation at 365 nm, three ruthenium complexes were found to promote the cleavage of plasmid pBR322 DNA from super coiled form ? to nicked form ??. Further in the presence of Co²⁺, the emission of DNA–Ru(ΙΙ) complexes can be quenched. And when EDTA was added, the emission was recovered. The experimental results show that all three complexes exhibited the “on–off–on” properties of molecular “light switch”. The highest Cytotoxicity potential of the complex1 was observed on the Human alveolar adenocarcinoma (A549) cell line. Good agreement was generally found between the spectroscopic techniques and molecular docked model which provides further evidence of groove binding.     

An efficient computational method for a stochastic dynamic lot-sizing problem under service-level constraints

We provide an efficient computational approach to solve the mixed integer programming (MIP) model developed by Tarim and Kingsman [8] for solving a stochastic lot-sizing problem with service level constraints under the static-dynamic uncertainty strategy. The effectiveness of the proposed method hinges on three novelties: (i) the proposed relaxation is computationally efficient and provides an optimal solution most of the time, (ii) if the relaxation produces an infeasible solution, then this solution yields a tight lower bound for the optimal cost, and (iii) it can be modified easily to obtain a feasible solution, which yields an upper bound. In case of infeasibility, the relaxation approach is implemented at each node of the search tree in a branch-and-bound procedure to efficiently search for an optimal solution. Extensive numerical tests show that our method dominates the MIP solution approach and can handle real-life size problems in trivial time.     

Physical, structural, and luminescence studies of Nd3+ doped MgO-ZnO borate glass

A series of borate glass of the system xNd₂O₃-5MgO-20ZnO-(75 ? x)B₂O₃, where x = 0.5, 1.0,1.5, 2.0, and 2.5 was successfully fabricated using melt quench method. The properties of the glass were characterized using X-ray diffraction (XRD), Fourier transform infrared (FTIR), absorption and luminescence spectra. The upconversion properties of Nd³⁺ doped borate glass were observed by using 574 nm excitation wavelength corresponding to ⁴ I _15/2 → ² H _114/2 transition. The emission bands centered at 460, 500 and 620 nm which corresponding to the Nd³⁺ transitions, ⁴ F _7/2 → ⁴ I _15/2, ² H _11/2 → ⁴ I _15/2, and ⁴ F _9/2 → ⁴ I _15/2 respectively were observed at room temperature. The presence of Nd³⁺ in borate based glass could intensify the upconversion luminescence spectra as it can potentially be used as host materials for upconversion lasers.