An immunoassay for terbutryn using direct hapten linkage to a glutaraldehyde network on the polystyrene surface of standard microtiter plates

2-Aminobutylamino-4-ethylamino-6-isopropylamino-1,3,5-triazine (ABA-atrazine) has been synthesized and used as a coating hapten in an immunoassay with a monoclonal antibody against terbutryn. Coating was achieved by covalently linking ABA-atrazine to a glutaraldehyde polymer network directly bound to the polystyrene surface of a standard 96-well microtiter plate. The assay was carefully optimized. In particular, the coating hapten concentration had a strong effect on the ELISA sensitivity. By including a pre-incubation step a low test midpoint (IC50-value) of 0.130 microg L(-1) was achieved. As far as we are aware this is the most sensitive ELISA for terbutryn yet reported. The coating-hapten-format presented is proposed as generally applicable, because the glutaraldehyde-modified microtiter plate surface enables stable immobilization of a broad variety of coating haptens.     

Measurement of GEp/GMp via polarization transfer at Q2 = 0.4 GeV /c2

The polarization transfer from longitudinally polarized electrons to protons in the elastic scattering p( e, e' p) has been measured around Q² = 0.4 (GeV/c)² with the three-spectrometer facility at the Mainz microtron MAMI. From this polarization transfer the ratio G _Ep/(G _Mp/μ_p) has been determined. The ratio is found to be slightly less than unity in agreement with recent results from other laboratories and from the Rosenbluth separation of cross-sections measured with unpolarized electrons. Received: 26 July 2001 / Accepted: 25 September 2001     

解读集成电路的无铅标签

给无铅器件打上标签在任何RoHS项目中都是一个关键部分,需要对部件和包装盒的标号多加注意。有时虽然部件的编号系统已经变化,生产商却仅在外包装盒上标识出来。     

Characterisation of 13C implantations in silicon by NRA [13C(p,γ)14N] and RBS

SiC_X layers close to the surface have been produced by implanting 40 keV ¹³C ions into silicon with a fluence of 6×10¹⁷ at./cm² (j=12 μA/cm²) at room temperature (RT). Depth distributions and areal densities (doses) of the implanted carbon have been analysed by the nuclear reaction ¹³C(p,γ)¹⁴N (NRA) which shows a sharp resonance in the excitation function at a proton energy of 1748 keV (Γ=75 eV FWHM). The depth resolution at the surface amounts to 31 nm due to energy spread of the proton beam (1.2 keV FWHM) and resonance width. The surface resolution of the NRA can be increased up to 8 nm when tilting the sample (surface normal) to an angle of 75° with respect to the proton beam direction. Using a NaI detector the detection limit of ¹³C in silicon is approximately 1 at.%. Comparative elastic backscattering measurements with ⁴He⁺ projectiles were performed at 2 MeV (Rutherford backscattering spectroscopy, RBS) and 3.45 MeV (high energy backscattering, HEBS) at a backscattering angle of 171°. The measured ¹³C depth distributions have been compared with a distribution calculated by the Monte Carlo algorithm T-DYN.     

Matrix isolation and density functional theory study of bis(trifluoromethyl)dioxodiazine: a photodimer of trifluoronitrosomethane

Trifluoronitrosomethane (CF3NO) was trapped in rare gas matrixes and irradiated at 633 and 670 nm. The infrared spectra of the postirradiation samples exhibit features consistent with cis and trans conformers of bis(trifluoromethyl)dioxodiazine, a previously uncharacterized species. The concentration dependence of the formation of the dimer is consistent with a mechanism in which monomers trapped in adjacent sites undergo excitation and subsequent reaction. The dimers reversibly form the monomer when irradiated with ultraviolet light. Density functional theory was used to determine the structure of the dimers and predict their infrared and Raman spectra. The predicted vibrational frequencies are in agreement with those observed. A third (skewed) conformation was predicted to have a triplet ground state, but no evidence of this species was observed. All three dimers exhibit significant diradical character, as evidenced by comparatively low N-N and high N-O stretching frequencies. Transition-state calculations predict the dimerization barrier to range from 17.1 (cis) to 35.0 (trans) kJ mol(-1) for the singlet dimers and to be 62.1 kJ mol(-1) for the triplet dimer. This is an example of nitroso dimerization that requires electronic excitation to proceed.     

Characterization of copolymers of polycarbonate and polydimethylsiloxane by 2D chromatographic separation,MALDI-TOF mass spectrometry,and FTIR spectroscopy

Abstract

The structure and composition of polycarbonate polydimethylsiloxane copolymer (PC-co-PDMS) was investigated by applying various analytical approaches including chromatographic separation methods, spectrometric, and spectroscopic detection techniques. In particular, size exclusion chromatography (SEC) and liquid adsorption chromatography operating at different conditions (e.g. using gradient solvent systems) were used to achieve separations according to molar mass and functionality distribution. The coupling of both techniques resulted in fingerprint two-dimensional plots, which could be used to easily compare different copolymer batches. Matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry was applied for structural investigations. The different ionization behavior of both comonomers, however, strongly limited the applicability of this technique. In contrast to that, Fourier-transform Infrared (FTIR) spectroscopy could be used to quantify the amount of PDMS in the copolymer at different points in the chromatogram. The resulting methodology was capable of distinguishing PC-co-PDMS copolymer from PC homopolymer chains present in the material.     

Die spektrophotometrische Bestimmung der mit Glycin oder Taurin konjugierten Desoxychols?ure

Ohne Zusammenfassung     

Die titrimetrische Bestimmung des Diacetylmonoxims

Ohne Zusammenfassung     

BSA adsorption on differently charged polystyrene nanoparticles using isothermal titration calorimetry and the influence on cellular uptake

BSA adsorption onto negatively and positively charged polystyrene nanoparticles was investigated. The nanoparticles were characterized in terms of particle size, zeta potential, surface group density, and morphology. The adsorption behavior of BSA on the particle surface, as a function of pH and overall charge of the particle, was studied using ITC. Different thermodynamic data such as enthalpy changes upon binding and stoichiometry of the systems were determined and discussed. The degree of surface coverage with BSA was calculated using the thermodynamic data. The cellular uptake of particles before and after BSA adsorption was studied using HeLa cells in the presence and absence of supplemented FCS in the cell culture medium.