This article reports on the development of a simple and cost-effective bioassay for the detection of biotin in urine and serum, based on the very selective binding of avidin and biotin. Avidin was allowed to react without isolating it from egg white. Egg white was treated with the dye HABA, which binds to avidin. Upon subsequent treatment with biotin, HABA is released due to the high affinity of biotin to avidin. The amount of HABA released is proportional to the amount of biotin used. 相似文献
All freely available plane-of-array (POA) transposition models and photovoltaic (PV) temperature and performance models in pvlib-python and pvpltools-python were examined against multiyear field data from Albuquerque, New Mexico. The data include different PV systems composed of crystalline silicon modules that vary in cell type, module construction, and materials. These systems have been characterized via IEC 61853-1 and 61853-2 testing, and the input data for each model were sourced from these system-specific test results, rather than considering any generic input data (e.g., manufacturer's specification [spec] sheets or generic Panneau Solaire [PAN] files). Six POA transposition models, 7 temperature models, and 12 performance models are included in this comparative analysis. These freely available models were proven effective across many different types of technologies. The POA transposition models exhibited average normalized mean bias errors (NMBEs) within ±3%. Most PV temperature models underestimated temperature exhibiting mean and median residuals ranging from −6.5°C to 2.7°C; all temperature models saw a reduction in root mean square error when using transient assumptions over steady state. The performance models demonstrated similar behavior with a first and third interquartile NMBEs within ±4.2% and an overall average NMBE within ±2.3%. Although differences among models were observed at different times of the day/year, this study shows that the availability of system-specific input data is more important than model selection. For example, using spec sheet or generic PAN file data with a complex PV performance model does not guarantee a better accuracy than a simpler PV performance model that uses system-specific data. 相似文献
The aim of this study was to propose a new conceptualization of early number sense. Six-year-old students’ (n = 204) number sense was tracked from the beginning of Grade 1 through the beginning of Grade 2. Data analysis suggested that elementary arithmetic, conventional arithmetic, and algebraic arithmetic contributed to the latent construct early number sense, and the invariance of the model over time was validated empirically. Algebraic arithmetic represents the dimension of early number sense that moves beyond conventional arithmetic and encompasses an abstract understanding of the relations between numbers. A parallel process growth model showed that the three components of number sense adopt a linear growth rate. A structural model showed that the growth rate of the algebraic arithmetic component has a direct effect on the growth rate of conventional arithmetic, and subsequently the growth rate of conventional arithmetic predicts the growth rate of elementary arithmetic. 相似文献
Steady convective mass transfer to or from fluid interfaces in pores of angular cross-section is theoretically investigated.
This situation is relevant to a variety of mass transport process in porous media, including the fate of residual non-aqueous
phase liquid ganglia and gas bubbles. The model incorporates the essential physics of capillarity and solute mass transfer
by convection and diffusion in corner fluid filaments. The geometry of the corner filaments, characterized by the fluid–fluid
contact angle, the corner half-angle and the interface meniscus curvature, is accounted for. Boundary conditions of zero surface
shear (‘perfect-slip’) and infinite surface shear (‘no-slip’) at the fluid–fluid interface are considered. The governing equations
for laminar flow within the corner filament and convective diffusion to or from the fluid–fluid interface are solved using
finite-element methods. Flow computations are verified by comparing the dimensionless resistance factor and hydraulic conductance
of corner filaments against recent numerical solutions by Patzek and Kristensen (J. Colloid Interface Sci 236, 305–317 2001). Novel results are obtained for the average effluent concentration as a function of flow geometry and pore-scale
Peclet number. These results are correlated to a characteristic corner length and local pore-scale Peclet number using empirical
equations appropriate for implementation in pore network models. Finally, a previously published “2D-slit” approximation to
the problem at hand is checked and found to be in considerable error. 相似文献
The various end‐to‐end distances of four‐junction polymers are investigated. The sizes of the different kinds of equal length branches and the backbone of two different polymers, with either nine or eleven branches, are estimated by means of both renormalization‐group and MC calculations. The comparisons of first‐order ε = 4 − d predictions with the MC results are satisfactory. The same trends are present in both techniques. The excluded‐volume interactions from additional branches further expand the various parts of the chains so that internal branches are larger than external ones. The branch ratios in the eleven‐branch case are expanded even more than the corresponding ratios of the nine‐branch polymer.
Diosmetin and hesperetin are the aglycones of the flavonoid glycosides diosmin and hesperidin which occur naturally in citrus fruit. A GC/MS method for the simultaneous determination of diosmetin and hesperetin in human plasma and urine has been developed and validated. The method was linear in the 2-300 ng/mL concentration range for both diosmetin and hesperetin in plasma and urine (r > 0.999). The precision of the method was better than 6.01 and 7.16% for diosmetin and hesperetin, respectively, and the accuracy was 96.76-100.40% and 95.00-105.50% for diosmetin and hesperetin, respectively. The lower limit of quantitation was found to be 2 ng/mL for both analytes in plasma and urine. Recovery of diosmetin, hesperetin and internal standard naringenin was greater than 82.5%. The method has been applied for the determination of diosmetin and hesperetin in plasma and urine samples obtained from a healthy male subject following a single oral 1000 mg dose of the flavonoid glycoside diosmin. The presence of hesperetin in plasma and urine samples indicates the metabolic reduction of diosmetin to its flavanone analogue hesperetin through reduction of the 2,3 double bond of the C-ring by the enzymes of bacteria of the intestinal microflora. 相似文献
A critical problem in building long systolic arrays lies in efficient and reliable synchronization. We address this problem in the context of synchronous systems by introducing probabilistic models for two alternative clock distribution schemes: tree and straight-line clocking. We present analytic bounds for the Probability of Failure and the Mean Time to Failure, and examine the trade-offs between reliability and throughput in both schemes. Our basic conclusion is that as the one-dimensional systolic array gets very long, tree clocking becomes more reliable than straight-line clocking. 相似文献
