Conformational Transformation Exhibited by the Peptide Extracted from Crystalline Region of Bombyx mori Silk Fibroin in Solid and Solution States

The conformational transformation of a 30-residue peptide H（Ala-Gly-Ser-Gly-AIa-Gly）5OH, i.e., （AGSGAG）5, extracted from highly crystalline region of Bombyx mori （B. mori） silk fibroin was described by using the high resolution solid state 13^C NMR, and CD spectroscopies. Based on the conformation-dependent 13^C NMR chemical shifts of the Ala, Gly and Ser residues and the line-shape analysis of the conformation sensitive Ala Cβ resonance, the peptide revealed a strong preference for silk Ⅱ structural form, i,e,, an antiparallel fl-sheet structure （φ= - 140±20°and ψ= 135±20°） in solid state. On the contrary, the CD spectra of this peptide in the two non-native hexafluorinated fibre spinning solvents, hexafluoroisopropanol （HFIP） and hexafluoroacetone （HFA）, exhibited the existence of an unusual tightly-folded conformation resembling 310-helix （φ=- 60±20° and ψ=-30±20°）, as judged from the R ratio of [θ]222/[θ]203 in HFIP solution, whereas a dynamically averaged unordered structure in HFA, Taken together, the information inclined to hypothesis that the primary structure of the highly crystalline regions of B. mori silk fibroin may be easily accessible to the large conformational changes, which in turn may be critical for facilitating the structural transformation from unprocessed silk fibroin （silk I form） to processed silk fiber （silk Ⅱform）.     

基于自校准技术的半球谐振陀螺正交误差的辨识补偿

在静电刚度校正技术的基础上,结合自校准算法,该文提出了一种实时辨识补偿正交误差的理论模型,并在两件套平面电极的半球谐振陀螺上进行了理论仿真和实验验证。结果表明,该方法不仅适用于半球谐振陀螺的力平衡和全角两种工作模式,还适用于虚拟进动的情况。此外,该文提出的补偿方法不会影响正交环路的控制精度,且对陀螺检测和驱动电路的增益和相位误差不敏感。     

提升配电自动化课程内涵的实验平台建设

配电自动化作为新工科智能电网的主要内容,在实验教学上还不满足新工科内涵要求。为加强学生在配电网方面的实践能力,提出配电自动化实验教学体系及课程教学的改革方案,基于贵州大学自主研发的实时仿真器UREP、嵌入式开发系统及PC端搭建配电网模块化半实物通用实时仿真实验平台。以馈线自动化为例,阐述平台的使用方法及步骤。提升学生理论与实践相结合的能力,达到新工科人才培养目的。     

一种6~18 GHz宽带高精度有源移相器

设计了一种6 bit 6~18 GHz工作频段的宽带高精度有源移相器。片上集成了输入无源巴伦、逻辑编码器、RC多相滤波器、矢量合成单元、数控单元等。该移相器的设计采用55 nm CMOS工艺实现,芯片尺寸为1.29 mm×0.9 mm,移相器核心尺寸为1.02 mm×0.58 mm。后仿结果表明,在6~18 GHz频率范围内,增益误差RMS值小于1 dB,相位误差RMS值小于0.75°,输入回波损耗、输出回波损耗分别小于-8.5 dB、-8.9 dB,芯片总功耗为20.7 mW。该6 bit移相器的相对带宽为100%,覆盖C、X和Ku波段,适用于雷达探测等领域。     

能量收集中继安全传输网络的在线功率控制算法

针对源节点和中继节点均采用收集能量供电的放大转发中继网络,考虑两个目的节点之间信息相互保密的场景,该文提出最大化长期时间平均保密速率的源节点和中继节点发送功率联合优化算法.由于能量到达和信道状态是随机过程,该问题是一个随机优化问题.利用Lyapunov优化框架将电池操作和能量使用约束下的长期优化问题转化为每时隙的"虚队列漂移加惩罚"最小化问题,并求解.仿真结果显示该文提出的算法在长期平均保密速率上相较于对比算法具有显著的优势,同时算法仅依赖于当前的电池状态和信道状态信息做出决策,是一种实用的、低复杂度的算法.     

ISOLATION,CHARACTERIZATION AND EXPRESSION OF THE COMPLEMENTARY DNA FOR HUMAN TUMOR NECROSIS FACTOR (TNF-α)

A cDNA for human TNF-α (615bp) was isolated by means of polymerase chain reaction (PCR) using first strand cDNA from PMA-induced HL-60 cells as template. The result from sequencing the 615 bp cDNA fragment indicated that it corresponded to the entire sequence of mature human TNF coding region. Direct expression of mature human TNF was achieved using a plasmid pHT-1 constructed by ligation of the cDNA and a synthetic DNA. The IPTG-induced bacterial product (hTNF) showed cytotoxicity to mouse L-929 cells. The TNF activity was further identified by neutralization of a specific monoclonal antibody against human TNF-α. Approximately 80,000 units of activity were detected per ml of culture at A600=2.     

掺杂态聚苯胺的共振拉曼光谱研究

观测了聚苯胺(PAn)在不同状态(掺杂、反掺杂、碱式、氧化、还原)下的拉曼光谱.根据其光谱特性,结合其它实验结果,提出了掺杂态PAn分子链包含电荷分布不等的三种苦环和二种氮原子的四单元苯醌变体模型,指出质子酸掺杂系分子内氧化还原反应,讨论了PAn氧化程度对掺杂效率的影响.     

透射电镜像转角的测定及应用

论述了完全标定透射电镜像转角所需拍摄的二次曝光电子显微像的数量,推导了由已测的像转角间接计算其它像转角的一般公式,以Philips Cm200电镜为实例,说明了测定像转角的基本步骤,并以实例说明怎样利用像转角,把衍射花样的晶体学信息传递给电子显微像。