锁核酸分子信标在分子识别与生物分析中的应用

分子信标是一种荧光探针,闭合时呈发夹结构。其5'末端修饰荧光基团,3'末端修饰猝灭基团。当目标存在时,环部与目标结合,发夹打开,发出荧光。锁核酸是一类双环状寡核苷酸衍生物,能够遵循碱基互补配对原则与核酸结合。锁核酸分子信标技术,结合了分子信标无需分离未结合探针而直接检测的优势和锁核酸亲合力强、热稳定性好、抗酶切以及体内无毒等特点,在核酸检测方面具有灵敏度高、特异性好的独特优势,近年来得到广泛关注。本文介绍了锁核酸修饰分子信标的结构、功能、设计要点,及其研究现状和一些重要进展,并讨论了目前锁核酸分子信标在分子识别及生物分析中的应用及存在的问题和发展前景。     

Elastic Low-Energy Electron Collisions with Methanethiol

We report ab initio scattering calculations results of electron-methanethiol collisions using R-matrix approach within the static-exchange and static-exchange-polarization approximations. The calculated elastic integral cross sections are in agreement with the experimental data. Two shape resonances of ²A' symmetry located at 4.06 and 8.32 eV are detected within static-exchange-polarization model. For this dipole molecule, Born-closure procedure was used to account for the higher partial waves (l>4) for the convergence of the cross section. The effective collision frequencies over a wide electron temperature range (200?30000 K) are calculated using the data of the momentum-transfer cross section.     

The Role of Taurine in Mitochondria Health: More Than Just an Antioxidant

Taurine is a naturally occurring sulfur-containing amino acid that is found abundantly in excitatory tissues, such as the heart, brain, retina and skeletal muscles. Taurine was first isolated in the 1800s, but not much was known about this molecule until the 1990s. In 1985, taurine was first approved as the treatment among heart failure patients in Japan. Accumulating studies have shown that taurine supplementation also protects against pathologies associated with mitochondrial defects, such as aging, mitochondrial diseases, metabolic syndrome, cancer, cardiovascular diseases and neurological disorders. In this review, we will provide a general overview on the mitochondria biology and the consequence of mitochondrial defects in pathologies. Then, we will discuss the antioxidant action of taurine, particularly in relation to the maintenance of mitochondria function. We will also describe several reported studies on the current use of taurine supplementation in several mitochondria-associated pathologies in humans.     

端面抽运Tm，Ho：YLF激光器双稳特性的理论分析与实验研究

报道了波长为792nm激光二极管端面抽运Tm,Ho：YLF连续激光器的双稳输出特性.在考虑能量传递上转换和基态对2μm激光重吸收的前提下,建立了Tm,Ho：YLF双稳激光器准三能级速率方程理论模型.通过对Tm,Ho：YLF激光器速率方程的求解,理论预见了Tm,Ho：YLF激光器双稳输出的特性,并分析了其产生的机理.进行了激光二极管端面抽运Tm,Ho：YLF激光器双稳输出的实验研究,当激光晶体温度为253K时,双稳区的宽度可达1.6W,跃变点输出功率的跃变量可达82.5mW.通过对比发现理论结果与实验结果比 关键词： 端面抽运 Tm Ho：YLF晶体 连续输出 光学双稳     

Pigment identification and decoration analysis of a 5th century Chinese lacquer painting screen: a micro‐Raman and FTIR study

To investigate the pigments and decoration applied to a wood‐based lacquer painting screen from the tomb of Si‐ma Jin‐long, Shanxi Province, central China, made by Chinese craftsmen in the 5th century, a combination of micro‐Raman spectroscopy, Fourier transform infrared spectroscopy (FTIR), wavelength dispersive X‐ray fluorescence (WDXRF), and microscopic examination was used. The obtained results are as follows: (1) the black, yellow, and red colors are identified as carbon black, orpiment and realgar, and cinnabar, respectively, by using micro‐Raman spectroscopy. The FTIR result shows that the white pigment filled in the leaves is not lead white, as assumed in the literature, but gypsum. Whether lead white was used at other locations remains unanswered and requires more samples for further work; (2) the thickness of each discernable pigment layer, as observed under the microscope, is approximately equal and the differences among them are small, suggesting a superfine painting skill; besides, a noticeable smooth interface between wood and the red grounding substance indicates that a polishing process might have been applied before the painting; (3) the red background was proved to contain cinnabar, but further FTIR analyses found no evidence for the presence of Chinese wood oil; and (4) the most interesting finding, rarely reported before, is that white grains of different sizes are found in both pigment layers and the grounding substance, which are perhaps an intentional addition. Further, in situ XRF and Raman analyses indicate that they are sourced from hydroxyapatite, coming probably from the intentional addition of animal bone ash to the lacquer. But how such a process could be finished and what purpose it served have not yet been answered. Copyright © 2009 John Wiley & Sons, Ltd.     

Periodic solutions for planar four-body problems

For Newtonian four-body problems with equal masses, we use variational minimizing methods to prove the existence of non-collision periodic solutions such that all bodies move on two symmetric trajectories with opposite orientation; here the methods and results are simpler and more general than those of Chen [K.-C. Chen, Action minimizing orbits in the parallelogram four-body problem with equal masses, Arch. Ration. Mech. Anal. 158 (2001) 293–318].     

6.17-W continuous wave operation of a 2132-nm c-cut Tm,Ho:YAP pumped by a laser diode of 794.3 nm

Continuous wave (CW) operation of a c-cut Tm (5 at %), Ho (0.3 at %):YAP laser at 2132 nm wavelength were reported in this paper. In the temperature of 77 K, the Tm,Ho:YAP crystal was double end-pumped by a 21.4 W fiber-coupled laser diode (LD) at the center wavelength of 794.3 nm. Different resonator lengths and output couplers for the total pump power were tried. The resonator length was 205 mm, and the maximum CW output power of 6.17 W was acquired, corresponding to an optical-optical conversion efficiency of 28.8% and a slope efficiency of 35.6%.     

Short-term frequency characteristics of 2 µm single frequency Solid-state lasers

The Short term frequency stability characteristics of 2 μm single frequency Solid-state lasers was investigated. The two laser systems of 2 μm single longitudinal mode oscillation Tm, Ho:YLF microchip laser and Ho:YAG NPRO laser were designed and constructed. The Short term frequency stability of these two laser were measured with the fiber delay self-beating heterodyne method. The 3dB width of the relative frequency fluctuation of Tm, Ho:YLF microchip laser and Ho:YAG NPRO laser were measured to be 895 and 736 Hz with 500 m fiber optical (2.5 μs delay). The proportional relation between the lasing fluctuation and the delay time were 358 and 263 Hz/μs, respectively. The vibration experiment was presented and it indicated that the NPRO Ho:YAG was more terrible to the influence of vibration, which is important in the practical application of wind measurement lidar.     

THG in dielectrics using low-energy tightly-focused ir femtosecond laser: third-order nonlinearity measurements and the evolution of laser-induced plasma

The third-order nonlinearity x ⁽³⁾ and other optical properties of LiF, CaF₂, fused silica, and free standing polymeric thin film are studied using a diagnostic technique based on the third-harmonic generation of tightly-focused femtosecond Cr:forsterite laser radiation. Time-resolved measurements of the plasma evolution in the bulk of a fused silica sample are performed by a pump-probe technique using the fundamental and third-harmonic radiation as a probe. With the use of the pump-probe technique, two types of the relaxation processes are registered.     

Electron swarm coefficients in 1,1,1,2 tetrafluoroethane (R134a) and its mixtures with Ar

Using a pulsed Townsend technique, we have measured the drift velocity, the longitudinal diffusion coefficient and the effective ionisation coefficient of electrons in R134a and R134a-Ar over a wide range of the density-reduced electric field intensity, E/N. Regarding the measurement of the electron drift velocities and of the effective ionization coefficients, we have covered a wider range than that hitherto achieved for pure R134a. Both the electron drift velocity and the effective ionisation coefficient have been found in very good agreement with those published in the literature, covering a shorter range of E/N. On the other hand, the swarm coefficients on R134a-Ar are, to the best of our knowledge, the first to be published. It is hoped that these data will be of interest for the test/derivation of electron collision cross sections for this important hydrofluorocarbon gas, which is nowadays of great use in gaseous detectors.