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141.
Laili Mahmoudian Jonas Melin Mohamad Reza Mohamadi Keiko Yamada Michio Ohta Noritada Kaji Manabu Tokeshi Mats Nilsson Yoshinobu Baba 《Analytical sciences》2008,24(3):327-332
We have developed a new method for a fast and precise analysis of circle-to-circle amplification (C2CA) product for specific gene detection by microchip electrophoresis. In this method, we have added a new enzymatic step to the C2CA reaction, which could be carried out isothermally at 37 degrees C. Compared to the original single-stranded DNA, the double-stranded DNA that is produced by this enzymatic reaction is more reliable for analysis by microchip electrophoresis. C2CA product was detected within 55 s with high reproducibility by this method which was successfully applied to the detection of 10-ng genomic DNA of the pathogenic bacteria Vibrio. cholerae within 110 s. Purification was found to be an indispensable step for the analysis of the C2CA product of genomic DNA samples. 相似文献
142.
The pressure dependences of the self-diffusion coefficients of deuterium oxide in 4.5m solutions of LiCl–D2O and CsCl–D2O (also 7m) and 3.06m CaCl2–D2O have been measured by the NMR spin-echo method at 30°C, 60°C, and 90°C. Shear viscosities and densities of these solutions have also been determined over the same range of experimental conditions. The experimental data show that the diffusion constantD decreases with the increasing structure-making ability of the electrolyte cation Ca+2>Li+. In contrast, the diffusion coefficient for D2O in the 4.5 and 7m CsCl solutions is equal to that for pure D2O at 30°C but lower at 60°C and 90°C. It has been found that the Stokes-Einstein equation relates well the diffusion coefficients to shear viscosity in these concentrated electrolyte solutions. 相似文献
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The history of microcomputer application in automotive electronics is briefly reviewed. American, Japanese, and European automobile manufacturer's microcomputer products are considered, and representative production designs are compared. Differences in approaches and problem solutions are examined. The chronological introduction of microcomputer-controlled functions is presented for engine, transmission, entertainment/comfort/convenience, and instrument panel control. Growth trends are noted for each category, and future automotive microcomputer applications are discussed. Projections are made of future semiconductor VLSI technology and automotive microcomputers. 相似文献
146.
The 23Na NMR lineshapes are reported for the ionic mesophase and isotropic phase of the melts of sodium n-butyrate and sodium isovalerate. The powder pattern for the central transition typical for the second-order quadrupole effect observed in the mesophase melts is of particular interest. Some analogies to 23Na behavior in sodium β-alumina are pointed out. 相似文献
147.
Anders Björner Andreas Paffenholz Jonas Sjöstrand Günter M. Ziegler 《Discrete and Computational Geometry》2005,34(1):71-86
In 1992 Thomas Bier presented a strikingly simple method to produce a
huge number of simplicial (n – 2)-spheres on 2n vertices, as deleted
joins of a simplicial complex on n vertices with its combinatorial
Alexander dual.
Here we interpret his construction as giving the poset of all the
intervals in a boolean algebra that “cut across an ideal.” Thus we
arrive at a substantial generalization of Bier’s construction: the
Bier posets Bier(P, I) of an arbitrary bounded poset P of
finite length. In the case of face posets of PL spheres this yields
cellular “generalized Bier spheres.” In the case of Eulerian or
Cohen–Macaulay posets P we show that the Bier posets Bier(P, I)
inherit these properties.
In the boolean case originally considered by Bier, we show that all the
spheres produced by his construction are shellable, which yields “many
shellable spheres,” most of which lack convex realization.
Finally, we present simple explicit formulas for the g-vectors of
these simplicial spheres and verify that they satisfy a strong form of
the g-conjecture for spheres. 相似文献
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