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111.
Wang B Shang JZ Qin YJ Yan BN Guo XH 《Journal of the American Society for Mass Spectrometry》2011,22(8):1453-1462
Electrospray ionization coupled with low energy collision induced dissociation (CID) in an ion trap mass spectrometer was
used to examine the fragmentation patterns of the [M + Na]+ of eight pairs of heptapeptides containing α- or β-Asp residues in second and sixth amino acid positions, respectively. Selective
cleavages at the peptide backbone C-terminal to two Asp residues were observed, which generated a series of C-terminal y5 ions and N-terminal b6 ions. Two typical ions:
[ \texty5 + \textNa-\textH ] + {\left[ {{{\text{y}}_{{5}}} + {\text{Na}}-{\text{H}}} \right]^{ + }} and
[ \textb6 + \textNa + \textOH ] + {\left[ {{{\text{b}}_{{6}}} + {\text{Na}} + {\text{OH}}} \right]^{ + }} , produced by α-Asp containing peptides were noted to be much more abundant than those of the peptides with β-Asp, which could
be used for distinction of the isomers in Asp2 and Asp6, respectively. In addition, a series of internal ions generated by
simultaneous cleavages at Asp residues were detected. Competitive reactions of carboxylic groups occurred between Asp6 side
chain and C-terminus. Formation mechanisms of most product ions are proposed. The results obtained in this work are significant
since low energy CID has been demonstrated to be effective for the distinction of Asp isomers. 相似文献
112.
Fragment density functional theory (DFT) calculation of NMR chemical shifts for several proteins (Trp-cage, Pin1 WW domain, the third IgG-binding domain of Protein G (GB3) and human ubiquitin) has been carried out. The present study is based on a recently developed automatic fragmentation quantum mechanics/molecular mechanics (AF-QM/MM) approach but the solvent effects are included by using the PB (Poisson-Boltzmann) model. Our calculated chemical shifts of (1)H and (13)C for these four proteins are in excellent agreement with experimentally measured values and represent clear improvement over that from the gas phase calculation. However, although the inclusion of the solvent effect also improves the computed chemical shifts of (15)N, the results do not agree with experimental values as well as (1)H and (13)C. Our study also demonstrates that AF-QM/MM calculated results accurately reproduce the separation of α-helical and β-sheet chemical shifts for (13)C(α) atoms in proteins, and using the (1)H chemical shift to discriminate the native structure of proteins from decoys is quite remarkable. 相似文献
113.
114.
A new neolignan glycoside from the leaves of Acer truncatum 总被引:1,自引:0,他引:1
A new neolignan glycoside, (7R,8R)-7,8-dihydro-9'-hydroxyl-3'-methoxyl- 8-hydroxymethyl-7-(4-hydroxy-3-methoxyphenyl)-1'-benzofuranpropanol 9'-O-beta-D- glucopyranoside (1) was isolated from the leaves of Acer truncatum along with (7R,8R)-7,8-dihydro-9'-hydroxyl-3'-methoxyl-8-hydroxymethyl-7-(4-O-alpha-L-rhamno- pyranosyloxy-3-methoxyphenyl)-1'-benzofuranpropanol (2), schizandriside (3), lyoniresinol (4), berchemol (5), (-)-pinoresinol-4-O-beta-D-glucopyranoside (6), hecogenin (7), chlorogenic acid (8) and neochlorogenic acid (9). Their structures were elucidated on the basis of extensive spectroscopic data. The absolute configuration of compounds 1 was established by its CD spectrum. The antibacterial activities of compounds 1-7 were evaluated. 相似文献
115.
A surfactant-sensitized spectrophotometric method for determination of trace orthophosphate has been developed using anion surfactant (Ultrawet 60 L) with molybdate and malachite green in low acidic medium (pHT 1.0). The method detection limit (3 × standard deviation of blank, n = 10) was 8 nM and the calibration curve was linear over a range of 10-400 nM (r2 = 0.997). The molar absorptivity was 1.26 × 105 L mol−1 cm−1 at 600 nm with the background correction at 530 nm. The precision of method was 3.4% at 50 nM and 2.4% at 100 nM orthophosphate (n = 10). The hydrolysis of eight organic phosphorus and polyphosphate compounds was less than 2% of the total phosphorus present (5-10 μM). This method showed less arsenate interference than previous methods, with only 3% even in the presence of orthophosphate in the samples. No interference of silicate up to 40 μM was observed. Background anions (in an order of SO42− > NO3− > Cl−) have greater effects than cations (Ca2+ > Mg2+ > Na+) on the reagent blank and the molar absorptivity of the color product. 相似文献
116.
Ablikim M Bai JZ Ban Y Bian JG Cai X Chen HF Chen HS Chen HX Chen JC Chen J Chen YB Chi SP Chu YP Cui XZ Dai YS Diao LY Deng ZY Dong QF Du SX Fang J Fang SS Fu CD Gao CS Gao YN Gu SD Gu YT Guo YN Guo YQ Guo ZJ Harris FA He KL He M Heng YK Hu HM Hu T Huang GS Huang XT Ji XB Jiang XS Jiang XY Jiao JB Jin DP Jin S Jin Y Lai YF Li G Li HB Li HH Li J Li RY Li SM Li WD Li WG Li XL Li XN Li XQ Li YL Liang YF Liao HB Liu BJ Liu CX Liu F Liu F Liu HH Liu HM Liu J Liu JB Liu JP Liu Q Liu RG Liu ZA Lou YC 《Physical review letters》2006,97(20):202002
Using a data sample of 58 x 10(6) J/psi decays collected with the Beijing Spectrometer II detector at the Beijing Electron Positron Collider, searches for invisible decays of eta and eta' in J/psi to phi eta and phi eta' are performed. The phi signals, which are reconstructed in K+K- final states, are used to tag the eta and eta' decays. No signals are found for the invisible decays of either eta or eta', and upper limits at the 90% confidence level are determined to be 1.65 x 10(-3) for the ratio B(eta-->invisible)/B(eta --> gamma gamma) and 6.69 x 10(-2) for B(eta' --> invisible)/B(eta' --> gammagamma). These are the first searches for eta and eta' decays into invisible final states. 相似文献
117.
We applied hydrostatic pressure (up to 10 GPa) to single-walled carbon nanotube bundles at low temperature (down to 2 K) to measure their magnetoresistance (MR) in a field up to 12 T. We found a pressure-induced transition in MR from positive to negative in the high-field regime. The onset of the transition occurs at approximately 1.5 GPa, which correlates closely with the tube shape transitions. The characteristics of the high-pressure MR are consistent with a model of pressure-induced two-dimensional weak localization. 相似文献
118.
Ablikim M Bai JZ Ban Y Cai X Chen HF Chen HS Chen HX Chen JC Chen J Chen YB Chi SP Chu YP Cui XZ Dai YS Deng ZY Dong LY Dong QF Du SX Du ZZ Fang J Fang SS Fu CD Gao CS Gao YN Gu SD Gu YT Guo YN Guo YQ Guo ZJ Harris FA He KL He M Heng YK Hu HM Hu T Huang GS Huang XP Huang XT Ji XB Jiang XS Jiao JB Jin DP Jin S Jin Y Lai YF Li G Li HB Li HH Li J Li RY Li SM Li WD Li WG Li XL Li XQ Li YL Liang YF Liao HB Liu CX Liu F Liu F Liu HH Liu HM Liu J Liu JB Liu JP Liu RG Liu ZA Lu F Lu GR Lu HJ Lu JG 《Physical review letters》2006,97(14):142002
A broad peak is observed at low K+K- invariant mass in J/psi-->K+K-pi(0) decays found in a sample of 5.8x10(7) J/psi events collected with the BESII detector. The statistical significance of the broad resonance is much larger than 5sigma. A partial wave analysis shows that the J;{PC} of this structure is 1--. Its pole position is determined to be [1576(-55)(+49)(stat)-91+98(syst)] MeV/c(2)-i/2[818(-23)(+22)(stat)-133+64(syst)] MeV/c(2). These parameters are not compatible with any known meson resonances. 相似文献
119.
120.
Tsai JZ Will JA Hubbard-Van Stelle S Cao H Tungjitkusolmun S Choy YB Haemmerich D Vorperian VR Webster JG 《IEEE transactions on bio-medical engineering》2002,49(5):472-483
We used a four-terminal plunge probe to measure myocardial resistivity in two directions at three sites from the epicardial surface of eight open-chest pigs in-vivo at eight frequencies ranging from 1 Hz to 1 MHz. We calibrated the plunge probe to minimize the error due to stray capacitance between the measured subject and ground. We calibrated the probe in saline solutions contained in a metal cup situated near the heart that had an electrical connection to the pig's heart. The mean of the measured myocardial resistivity was 319 ohm x cm at 1 Hz down to 166 ohm x cm at 1 MHz. Statistical analysis showed the measured myocardial resistivity of two out of eight pigs was significantly different from that of other pigs. The myocardial resistivity measured with the resistivity probe oriented along and across the epicardial fiber direction was significantly different at only one out of the eight frequencies. There was no significant difference in the myocardial resistivity measured at different sites. 相似文献