Stable Encapsulation of QD Barcodes with Silica Shells

Quantum dot-doped mesoporous microbeads (QDMMs) are encapsulated with silica shells for enhanced chemical stability. The results show that a micro-emulsion procedure is highly efficient in coating QDMMs with polyvinyl alcohol (PVA), which is important in the subsequent deposition of a silica shell. Incorporation of fluorescent silane precursors allows direct observation of silica shells by fluorescence microscopy. The resulting silica coated QDMMs (QDMM@SiO(2)) exhibit remarkable stability against solvent-induced QD leaching and chemical-induced fluorescence quenching compared with uncoated QDMMs. Further development of this technology such as optimization of silica shell thickness, surface modification with non-fouling polymers, and conjugation with biomolecular probes will enable clinical translation of the optical barcoding technology for highly multiplexed detection and screening of genes and proteins.     

基于β-环糊精接枝聚L-谷氨酸星型聚合物的可注射水凝胶的制备与表征

采用Gadelle-Defaye法制备了全-6-氨基-β-环糊精[β-CD-(NH2)7],并用"Grafting-from"接枝法得到不同分子量的星型结构β-环糊精-g-聚L-谷氨酸(β-CD-g-PLGA).对β-CD-g-PLGA进行酰肼化改性后与醛基化海藻酸钠(ALG-CHO)通过席夫碱交联反应制备β-CD-g-PLGA/ALG水凝胶.研究了前驱体浓度以及β-CD-gPLGA分子量对β-CD-g-PLGA/ALG水凝胶性能的影响,并以疏水性辛伐他汀(SIM)为模型药物,研究了水凝胶对SIM的控制释放行为.     

共振散射光谱分析法测定环境水样中痕量汞

在pH 9.5的NH3-NH4C l缓冲溶液中,汞(Ⅱ)与双硫腙可形成能稳定存在的螯合物微粒,显示出共振散射效应。该微粒体系的最强共振散射峰在560 nm处,汞(Ⅱ)的质量浓度在0.028~6.140μg/mL范围内,共振散射与△I之间存在良好的线性关系,回归方程为△I=76.786ρ+2.7,相关系数为0.999 8,检出限0.012μg/mL Hg。利用共振散射测量水样中微量汞(Ⅱ),方法简单、灵敏度高、结果满意。     

Quantum wavepacket ab initio molecular dynamics: generalizations using an extended Lagrangian treatment of diabatic states coupled through multireference electronic structure

We present a generalization to our previously developed quantum wavepacket ab initio molecular dynamics (QWAIMD) method by using multiple diabatic electronic reduced single particle density matrices, propagated within an extended Lagrangian paradigm. The Slater determinantal wavefunctions associated with the density matrices utilized may be orthogonal or nonorthogonal with respect to each other. This generalization directly results from an analysis of the variance in electronic structure with quantum nuclear degrees of freedom. The diabatic electronic states are treated here as classical parametric variables and propagated simultaneously along with the quantum wavepacket and classical nuclei. Each electronic density matrix is constrained to be N-representable. Consequently two sets of new methods are derived: extended Lagrangian-QWAIMD (xLag-QWAIMD) and diabatic extended Lagrangian-QWAIMD (DxLag-QWAIMD). In both cases, the instantaneous potential energy surface for the quantum nuclear degrees of freedom is constructed from the diabatic states using an on-the-fly nonorthogonal multireference formalism. By introducing generalized grid-based electronic basis functions, we eliminate the basis set dependence on the quantum nucleus. Subsequent reuse of the two-electron integrals during the on-the-fly potential energy surface computation stage yields a substantial reduction in computational costs. Specifically, both xLag-QWAIMD and DxLag-QWAIMD turn out to be about two orders of magnitude faster than our previously developed time-dependent deterministic sampling implementation of QWAIMD. Energy conservation properties, accuracy of the associated potential surfaces, and vibrational properties are analyzed for a family of hydrogen bonded systems.     

压电复合元件耦合振动模态分析

一种用于单波束的压电复合元件,采用整体切割灌注的方法制备,由压电陶瓷和聚合物两种不同材质组成.为进一步提高压电复合元件基元的一致性,利用有限元分析软件ANSYS10.0对单波束压电复合元件进行耦合模态的特性分析,得出了耦合频率与聚合物密度、泊松比和基元结构之间的关系.实验结果表明,通过调整边缘基元的外形尺寸,可有效提高边缘基元的耦合频率,从而达到与中心基元一致.     

FOG制造工艺及其关键技术

介绍了ACF技术和FOG工艺(ACF预贴、预邦定、主邦定和检测);详细阐述了FOG制造工艺的关键技术压力控制、温度控制及高温状态下压头的平行度要求;通过图表和数据分析提出了相应的解决方案,合理的气路设计提高邦定压力的精度和脉冲控温技术,利用有限元设计软件进行压头结构设计.FOG制造工艺及其关键技术的研究对我们研发相关设备具有重要的指导意义.     

有限元在金属微结构电铸特性分析中的应用

为改善高深宽比金属微结构的电铸成型质量,采用有限元方法分析微细电铸的电场和流场的分布特点,及搅拌方式、电场和流场分布对微细电铸质量的影响。研究表明:搅拌对电铸区域的影响只集中在微区的入口部分,深度小于55μm,扩散过程成为金属沉积的限制性因素;电力线曲率随电铸深宽比增大而提高,导致电铸微区内金属离子传输能力不均匀。微细电铸过程中,搅拌速度提高并不能改善金属沉积的传质条件;辅助超声的复合搅拌在相同条件下能提高金属沉积速度,减少铸层缺陷、改善传质。实现了表面光滑、侧壁陡直的金属微结构的电铸成型。     

Formation Mechanism and Stability Study of Konjac Glucomannan Helical Structure

The formation mechanism and stability of konjac glucomannan (KGM) helical structure were investigated by molecular dynamic simulation and experimental method. The results indicate that the molecular conformation of KGM is a non-typical helical structure. In detail, helical structure of KGM is mainly sustained by acetyl group, whose size and stability are affected by the molecular polymerization degree of KGM. In vacuum among the non-bonding interactions, electrostatic force is the greatest factor affecting its helical structure, but in water solution, hydrogen bond affects the helical arrangement greatly. To our interest, temperature exhibits a reversible destroying effect to some extent; the helical structure will disappear completely and present a ruleless clew-like arrangement till 341 K. This work suggests that the method of combining molecular dynamic simulation and experiment tools can be effective in the study of KGM helical structure.     

新型室温酯化法接枝聚合物改性SiO_2纳米微粒

提出了一种在室温、大气环境等温和条件下通过酯化反应将端羧基聚合物链接枝到纳米SiO2微球表面从而制备有机/无机复合纳米微粒的新方法.该方法通过以下两个步骤得以实现,即第一,用3-环氧丙基三甲氧基硅烷对纳米SiO2微球表面进行改性处理,接着将引入到纳米SiO2表面的环氧基团转化为烷羟基基团;第二,通过引入到纳米SiO2微球表面的烷羟基与聚合物中的端羧基在室温下发生酯化反应,从而将聚合物接枝到纳米SiO2表面制得复合微球.利用XPS、FTIR、TEM和TGA等测试手段对纳米SiO2的改性过程以及聚合物接枝后得到的复合微球进行了表征.研究结果表明,该室温酯化接枝方法具有较高的接枝率,接枝到无机纳米微粒表面的聚合物占复合微球质量的55wt%～70wt%;接枝聚合物后,纳米SiO2微球的粒径从40nm增加到64～75nm,从而得到了以SiO2为核、以聚合物为壳的有机-无机复合微球.     

Motion of micrometer sized spherical particles exposed to a transient radial flow: attraction, repulsion, and rotation

It is now accepted that the physical forces in ultrasonic cleaning are due to strongly pulsating bubbles driven by the sound field. Here we have a detailed look at bubble induced cleaning flow by analyzing the transport of an individual particle near an expanding and collapsing bubble. The induced particulate transport is compared with a force balance model. We find two important properties of the flow which explain why bubbles are effectively cleaning: During bubble expansion a strong shear layer loosens the particle from the surface through particle spinning and secondly an unsteady boundary layer generates an attractive force, thus collecting the contamination in the bubble's close proximity.