DNA电化学生物传感器测定水中痕量铅

采用物理涂附法将单链DNA固定到金表面得到ssDNA/Au修饰电极。研究表明,该电极在pH 5,0.2 mol/L NaAc-HAc缓冲底液中,可用于测定水中痕量Pb2 。Pb2 以Pb-DNA络合物的形式吸附在电极上,以示差脉冲伏安法测定Pb2 ,在0.15 V(vsSCE)处有灵敏的氧化峰,其峰电流与Pb2 浓度在5.0×10-8~1.0×10-6mol/L范围内呈良好的线性关系,此法的检测限为2.0×10-8mol/L,该修饰电极的稳定性和抗干扰性都较好。用该电极对饮用水中的Pb进行检测,得到了满意的结果。     

GAS SORPTION AND TRANSPORT IN POLY (PHENYLENE OXIDE)(PPO)AND ARYL-BROMINATED PPO MEMBRANES

The apparent solubility (S), concentration-average diffusivity (D), and permeability (P), for C0_2, CH_4 and N_2 through PPO and aryl-brominated PPO at 35℃for pressure ranging from 1 to 26 atm are reported. It is found that P, D, and S of the membranes to all the three gases vary with the extent of bromination. S increases with the increase of the percent of bromine in each case, but D to CO_2 increases remarkably only at higher degree of brominafion, and therefore, P to CO_2 is increased by more than 100% over a wide range ofpressure in the case. The solubility data are well described by the dual mode sorption model. It is found that the gas molecules sorbed by the Langmuir mode are relatively more immobilized and the contribution of the nonequilibrinm character of the polymer to gas permeation increases obviously for CO_2 and is hardly changed for CH_4 with increasing bromine content. These observations are interpreted in terms of changes in specific free volume (SFV)and the cohesive energy density (CED) of the polymers.     

肽核酸骨架分子键联卟啉的合成及结构表征

以5,10,15-三苯基-20-(4-羧基苯基)卟啉和5,10,15-三苯基-20-(4-羟基苯基)卟啉为原料, 分别与N-(Boc-氨乙基)甘氨酸乙酯(3)及其衍生物4作用, 得到了两种肽核酸骨架分子键联卟啉化合物6和8. 中间体和目标化合物均由紫外-可见光谱、红外光谱、核磁共振光谱、质谱及元素分析所确证. 目标化合物的荧光光谱测试结果表明, 肽核酸单元分子的链接对卟啉分子的荧光波长和强度影响不大.     

基于弹簧管悬臂梁的FBG压力传感的研究

提出了一种等强度悬臂梁与弹簧管结合的高灵敏度的光纤布拉格光栅(FBG)压力传感器。将FBG粘贴在悬臂梁的下表面,粘贴方向与悬臂梁轴线一致。理论分析了FBG中心波长相对偏移量与压力的关系,实验测得该传感器的压力灵敏度为2.767×10-4/MPa,是裸FBG压强灵敏度系数的142倍。压力灵敏度与悬臂梁和弹簧管的几何参数有关,改变参数,可获得不同灵敏度的压力传感器。     

晶体型二阶偏振模色散模拟器

报道了一种新型的二阶晶体型偏振模色散(PMD)模拟器,其由3片可产生不同的一阶PMD差分群延迟(DGD)的晶体和1个法拉第旋转器组成,法拉第旋转器理论上可在180°内连续变化。该二阶PMD模拟器实验上能近似模拟从50到200 ps2的二阶PMD。给出了二阶PMD随偏置电压变化的曲线。实验表明,该器件输出的重复性好,响应时间小于1 ms,实验值与理论值近似符合。     

光纤光栅传感器信号解调方法综述

当光纤光栅传感器上的温度或应力发生变化时,布拉格反射波长也随之发生漂移,根据这个漂移量便可判断待测量大小,因此,人们提出了许多解调方法.文章论述了几种常用的光纤光栅传感器信号解调方法,分类评述了这几种方法的工作原理和性能,分别给出了其典型的实验原理图,并对其优点和缺点进行了分析比较.     

一种用于波长路由光网络的分布式快速波长预留协议

波长预留协议用于在光通道建立过程中预留和配置波长.目前已有的波长预留协议都把协议设计的目标定为降低网络阻塞率,没有一种协议设计把目标定为缩短光通道的建立时间和简化协议处理过程.然而更短的建立时间和更小的协议复杂度对于未来极其动态的光网络却是非常重要的.提出了一种新颖的用于波长路由光网络的分布式快速波长预留协议-目的端预留协议,它在保证网络阻塞率可以接受的前提下,不仅可以降低连接建立时间,还可以减少控制节点处理器需要处理的信令消息数量.仿真结果证明了这一点.     

Screening of glycoside isomers in P. scrophulariiflora using ionic liquid‐based ultrasonic‐assisted extraction and ultra‐performance liquid chromatography/electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry

A powerful ionic liquid‐based ultrasonic‐assisted extraction (ILUAE) method combined with ultra‐performance liquid chromatography coupled to electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry (UPLC/ESI‐QTOFMSⁿ) was employed in the rapid simultaneous screening of iridoid glycosides, phenylethanoid glycosides, and cucurbitacin glycosides from P. scrophulariiflora. The ILUAE procedure was optimized over several ultrasonic parameters, including the ultrasonic power, concentration of the ionic liquid, and solid–liquid ratio. A comparison with conventional heat‐reflux extraction and regular UAE demonstrated that the optimized approach yielded a high extraction efficiency (Picroside I, 2.84%; Picroside II, 3.57%; 6‐O‐E‐feruloyl catalpol, 2.20%) within a short extraction time of 30 min. Negative ion mode ESI‐QTOFMS² analysis of the fragmentation reactions of the [M–H]^– ions was conducted to characterize the diagnostic ions related to the glycosyl moieties, aglycone units, and the type and substituted position of the ester groups. Interestingly, the positional isomers of the iridoid glycosides could be easily discriminated based on the characteristic ions. A total of 15 glycosides, including three groups of iridoid glycoside isomers and two groups of phenylethanoid glycoside isomers, were conveniently identified within 13.5 min. Moreover, 6'‐O‐vanilloyl catalpol was identified in P. scrophulariiflora for the first time. The method developed here was further validated by measuring the recovery, correlation coefficient (R²), and reproducibility (RSD, n = 5) of three iridoid glycosides: 89.60%–109.02%, 0.9991–0.9998, and 0.93%–1.44%, respectively. This study demonstrated the capabilities of ILUAE combined with UPLC/ESI‐QTOFMSⁿ for the rapid screening of glycosides in P. scrophulariiflora. This method offers an approach to similar studies on other natural plants. Copyright © 2012 John Wiley & Sons, Ltd.     

Risk and Intellectual Property in Technical Standard Competition： A Game Theory Perspective

Technical standard is typically characterized by network effect.The key point for a technical standard is the consumers＇ choice,which is based on consumers＇ maximum benefits.When a technical standard becomes a national standard,its interests have been integrated into the national interests.National interests are divided into economic profits and security factors.From the perspective of consumers＇ choice,this paper deals with the main factors which affect the result of technical standard competition- the risk and profits of intellectual property based on the assumption of bounded rationality and dynamic game theory.