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41.
Maria J. Matos Claudio D. Navo Tuuli Hakala Xhenti Ferhati Ana Guerreiro David Hartmann Barbara Bernardim Kadi L. Saar Ismael Compan Francisco Corzana Tuomas P. J. Knowles Gonzalo Jimnez‐Oss Gonalo J. L. Bernardes 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(20):6712-6716
Quaternized vinyl‐ and alkynyl‐pyridine reagents were shown to react in an ultrafast and selective manner with several cysteine‐tagged proteins at near‐stoichiometric quantities. We have demonstrated that this method can effectively create a homogenous antibody–drug conjugate that features a precise drug‐to‐antibody ratio of 2, which was stable in human plasma and retained its specificity towards Her2+ cells. Finally, the developed warhead introduces a +1 charge to the overall net charge of the protein, which enabled us to show that the electrophoretic mobility of the protein may be tuned through the simple attachment of a quaternized vinyl pyridinium reagent at the cysteine residues. We anticipate the generalized use of quaternized vinyl‐ and alkynyl‐pyridine reagents not only for bioconjugation, but also as warheads for covalent inhibition and as tools to profile cysteine reactivity. 相似文献
42.
The thermospray mass spectrometry (TSP/MS) of five N-methylcarbamates is presented. This is the first time that ions other than [M + H]+ and [M + NH4]+ have been reported using positive TSP/MS. Protonation of ROCONHCH3 yields the [CH3NH2CO]+· ion, with formation of the ion–molecule adduct [ROCONHCH3 · CH3NH2CO]+· through elimination of CO+· from [CH3NH2CO]+·, and the adduct [M + 75]+·, [ROCONHCH3 · OCONH2CH3]+·, is also obtained. 相似文献
43.
High-Resolution Temporo-Ensemble PIV to Resolve Pore-Scale Flow in 3D-Printed Fractured Porous Media
Ahkami Mehrdad Roesgen Thomas Saar Martin O. Kong Xiang-Zhao 《Transport in Porous Media》2019,129(2):467-483
Transport in Porous Media - Fractures are conduits that can enable fast advective transfer of (fluid, solute, reactant, particle, etc.) mass and energy. Such fast transfer can significantly affect... 相似文献
44.
We prove several Liouville theorems for F-harmonic maps from some complete Riemannian manifolds by assuming some conditions on the Hessian of the distance function, the degrees of F(t) and the asymptotic behavior of the maps at infinity. In particular, the results can be applied to F-harmonic maps from some pinched manifolds, and can deduce a Bernstein type result for an entire minimal graph. 相似文献
45.
R. Saar 《Fresenius' Journal of Analytical Chemistry》1924,65(1-2):61
Ohne Zusammenfassung 相似文献
46.
Wanda Wlostowska F. M. Wieninger H. Siegfried W. Schüler R. Saar H. Fink R. Lechner J. M. Guthrie G. G. Philip und Fr. Emslander 《Fresenius' Journal of Analytical Chemistry》1934,96(5-6):226-229
Ohne Zusammenfassung 相似文献
47.
48.
Localized structures in dryland vegetation: forms and functions 总被引:1,自引:0,他引:1
Vegetation patches in drylands are localized structures of biomass and water. We study these structures using a mathematical modeling approach that captures biomass-water feedbacks. Biomass-water structures are found to differ in their spatial forms and ecological functions, depending on species type, soil conditions, precipitation range, and other environmental factors. Asymptotic spot structures can destabilize to form ring structures, expanding in the radial direction, or crescent structures, migrating uphill. Stable spot structures can differ in their soil-water distributions, forming water-enriched patches or water-deprived patches. The various biomass-water structures are expected to function differently in the context of a plant community, forming landscapes of varying species diversity. 相似文献
49.
The density of states of a rectangular billiard with an Aharonov–Bohm flux line in its center was calculated in the semiclassical approximation and was used for the calculation of the form factor in the diagonal approximation. The distribution of nearest level spacings and the form factor were calculated also numerically. For some values of the flux these were found to be close to the ones of the semi-Poisson statistics. The difference between the numerical results and the semiclassical ones were found to be much larger than for chaotic and for integrable systems within similar approximations. Possible reasons for these discrepancies are discussed. 相似文献
50.