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Streak camera has high temporal resolution and high sensitivity, and is a powerful tool in biomedical study to measure fluorescence lifetime and perform fluorescence lifetime imaging. However, nonuniformity of the gain in the streak tube and nonlinearity of the sweeping speed limit the precision of fluorescence lifetime measurement, particularly when fluorescence lifetimes are short. We have constructed a two-photon excitation fluorescence lifetime measurement system that is based on a synchroscan streak camera and have developed accordingly a method to correct the effect of gain nonuniformity and nonlinearity of sweeping speed on the measurement precision. A continuous-wave laser of high stability is used to calibrate the gain of the streak camera, and a Fabry-Perot etalon is used to calibrate the nonlinearity of the sweeping speed. Fitting algorithms are used to correct the gain of the streak camera and nonlinearity of the sweeping speed respectively, which significantly improves the measurement precision of the system, as characterized through the fluorescence lifetime of the short-lived fluorescence dye, Rose Bengal. Experimental results show that the measurement fluctuation of the lifetime has been improved from more than 10% to 2% after correcting the effects of gain nonuniformity and sweeping speed nonlinearity. 相似文献
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We present a method for calibrating the position error of phase retrieval with transverse translation diversity in optical wavefront in situation where the position error sharply influences algorithm precision. This method involves testifying that the essence of the position error in phase retrieval is the translation in frequency domain and the minimum of iterative error against position error reaches when there is no position error. Then the least square method is used for fitting the relationship between the iterative error and the assumed position error in polynomial function. The computer simulations used to prove the validity of this method are described. The results indicated that this method can calibrate the position error to approximately 1/500 mm which nearly has no influence on the phase retrieval. 相似文献
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过渡金属醋酸盐与邻菲咯啉的室温固相配位化学反应研究 总被引:1,自引:0,他引:1
本文研究了C0(Ac)2·4H2O,Ni(Ac)24H2O,Cu(Ac)2·H2O,Zn(Ac)2·2H2O与邻菲咯啉在室温(20土2℃)条件下的固相配位化学反应,讨论了反应物分子结构对固相反应的影响,通过固相反应一步合成了相应的配合物,经元素分析、红外光谱,X-射线衍射测定,确定了固相合成产物的组成。 相似文献
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CHEN Wen-Sen South China Institute of Botany Chinese Academy of sciences Guangzhou Guangdong ChinaYAO Zhu-Jun ZHANG Yi-Bin XU Yong-Zhen WU Yu-LinState Key Laboratory of Bio-organic & Natural Products Chemistry Shanghai Instutute of Organic Chemistry Chinese Academy of Sciences Fenglin Lu Shanghai China 《中国化学》1995,13(3):263-266
Atemoyacin A, a new annonaceous acetogenin was isolated from the alcoholic extract of the seeds of Annona atemoya Hort (Annonaceae). Its structure and relative configuration were elucidated on the basis of the spectral analysis. 相似文献
90.
本文针对GSM业务管理中的编号计划、SIM卡管理和计费处理等与TACS系统差别较大的问题,介绍了相应的处理办法,旨在为GSM经营者在业务管理中提供有益的参考。 相似文献