Time-evolution-proof Scattering Data for the Focusing and Defocusing Zakharov-Shabat Systems

In this article we give sufficient conditions on the scattering data of a defocusing or focusing Zakharov-Shabat system in order that its potential is square integrable. For a dense subset of integrable as well as square integrable potentials, we show that the scattering data actually satisfy these sufficient conditions.     

A novel method for serum lipoprotein profiling using high performance capillary isotachophoresis

A new capillary isotachophoresis (cITP) method for lipoprotein profiling with superior lipoprotein coverage compared to previous methods has been developed, resolving twice as many lipoprotein species (18 peaks/fractions) in serum or plasma in less than 9.5 min. For this, a novel mixture of 24 spacers, including amino acids, dipeptides and sulfonic acids, was developed and fine-tuned, using predictive software (PeakMaster) and testing of spiked serum samples. Lipoprotein peaks were identified by serum-spiking with reference lipoproteins. Compatibility with common lipophilic stains for selective lipoprotein detection with either UV/Vis or laser-induced fluorescence was demonstrated. A special new capillary with a neutral coating (combining water-compatible OV1701-OH deactivation and methylation) was used for the first time for electrodriven separations, allowing very stable separations in a pH 8.8–9.4 gradient system, being functional for more than 100 injections. Excellent reproducibility was achieved, with coefficients of variation lower than 2.6% for absolute migration times. Comparison was performed with human plasma samples analyzed by NMR, leading to similar results with cITP after multivariate statistics, regarding group-clustering and lipoprotein species correlation. The new cITP method was applied to the analysis of serum samples from a LDL receptor knock-out mice model fed either a normal diet or a western-type diet. Differences in the lipoprotein levels and in the sublipoprotein types were detected, showing a shift to more atherogenic particles due to the high cholesterol diet. In summary, this novel method will allow more detailed and informative profiling of lipoprotein particle subtypes for cardiovascular disease research.     

Conformational Transmission in DNA

Abstract

This poster presents our recent results on DNA dimers in which a stable trigonal bipyramidal pentacoordinated phosphorus (P^v) structure forms the internucleoside linkage. Conformational analysis of the systems 1-4 with 300 and 500 ¹H NMR has shown that the P^v structure results in a distorted conformation of the backbone structuE (1).