蛙虹彩病毒cop基因的克隆表达及亚细胞定位

从蛙虹彩病毒(Rana gryliovirus,RGV)基因组中克隆了含凋亡相关结构域的新基因-cop(Caspaserecruit ment domain only protein,COP)基因的全部编码区,成功构建了重组表达载体,进行了原核表达,并在鲤鱼上皮瘤细胞(Epithelioma papulosumcyprini,EPC)中进行了亚细胞定位.序列分析表明,RGVcop基因全长288 bp,编码一个长为95 aa,分子量为10.4×103的推定蛋白.二级结构预测表明其含有5个α螺旋.同源性比对分析显示,RGVcop与其他6株虹彩病毒及人类cop相关蛋白同源性最高为94%,最低为33%.重组原核表达质粒pET28a-COP转化大肠杆菌BL21后,经IPTG诱导表达,获得一条约14×103的融合蛋白.重组真核表达质粒pEGFPN3-COP转染EPC细胞,经DAPI染色后,在荧光显微镜下观察显示重组蛋白在整个细胞中均有分布.     

In Situ Observation of Skeletal Shape Transition during BaB2O4 Crystal Growth in High-Temperature Solution

The transition from a fiat solid-liquid interface to a skeletal shape during BaB2O4 （BBO） single crystal growth in Li2B4O7 flux is observed in real time by an optical high-temperature in-situ observation system. The movement of crystal step is also investigated. The observation results demonstrate that the steps propagate along and parallel to the fiat interface when the crystal size is small. Nevertheless, they will ‘bend＇ close to the face centre if the crystal size becomes greater. Atomic force microscopy reveals that more deposition places near the face centre give rise to the bending of advancing steps and thus the formation of a vicinal interface structure. Measurements of step velocity show that the velocity keeps nearly constant at different moments for one specific step, whereas the step on a newly formed layer advanced faster than that on a previously formed one when the crystal size is larger than 210μm or so. Thus interracial morphological instability occurs and a skeletal interface is obtained.     

3mm波段极近距离雷达

提出一种极近距离雷达概念，并导出其作用距离公式，在此基础上研制出一台３ｍｍ波段极近距离多普勒雷达样机．试验结果表明该雷达结构简单，成本低廉，在作用距离为零点几米至几米范围内可获得较高的定距精度．     

一种新颖的半导体光放大器镀膜监控方法

本文介绍了一种在半导体光放大器镀膜过程中监测端面反射率的简单而有效的方法。理论模型的建立考虑了光放大器的漏电流和自发发射速率随载流于浓度的非线性变化，并且基于实验测量的ＰＩ（输出功率与注入电流）曲线，采用曲线拟合的方法来修正一些依赖于器件的参数，理论计算结果与有关文献的测量结果较好地吻合。     

SMT互连组件在热循环中的可靠性预计模型

电子设备中表面安装器件和电路基板以及焊接材料的热膨胀系数差异,使得SMT互连组件在热循环条件下产生热膨胀失配,在焊接处产生热应力,在长时间的交变应力的作用下,焊接处遭到疲劳破坏而使设备工作失效,这就是SMT互连组件在热循环条件下的基本失效机理。文中还介绍了美国MIL—HDBK—217F NOTICE Ⅱ公布的表面安装连接组件可靠性预测lSMT模型。给出一系列具有实用价值的参数。     

分形维数与城市规模分布评析

本在分形概念的基础上，指出城市规模分布是基于统计意义上的一种分形分布，并计算了安徽省1978～1998年城市规模分布的分维值，评析了安徽省城市规模分布变化的特征。     

Ka-band solid-state pulsed Gunn oscillator and power combiner

GaAs Gunn diodes were fabricated for pulse source application at 8 mm wave band and operated with pulsewidths of 0.05 to 2.0 microseconds and duty cycles of 0.001 to 0.01. Peak pulse output power levels of 0.8–1.2W are achieved and the maximum available power is 1.6W with the highest efficiency of 6.5 percent. A simple and compact pulsed power combiner is also given in this paper.     

信息高速公路上的安全问题

首先介绍了信息高速公路的概貌,并指出了信息高速公路将面临的威胁和攻击,接着说明了信息高速公路应当具备的基本安全特性以及实现这些特性可以采取的措施,最后介绍了信息高速公路安全技术的开发动态。     

SAW滤波器简化衍射频响的计算方法

提出了由一个变迹ＩＤＴ叉指换能器和一个均匀ＩＤＴ组成的ＳＡＷ滤波器简化衍射频响的计算方法，即将均匀ＩＤＴ简化成单根指计算其衍射频响。该方法与未简化衍射频响的计算方法相比，计算量大为减小，前者约为后者的（Ｎ为均匀ＩＤＴ的指对数）。并给出了一个实例，用未简化与简化方法分别计算了其衍射频响，结果表明，两者符合得较好。     

An electromigration failure model for interconnects under pulsedand bidirectional current stressing

An electromigration failure model which can be used to project the electromigration lifetime under pulsed DC and AC current stressing has been reported. The experimental results indicate that different metallization systems (Al-2%Si, Al4%Cu/TiW, and Cu) show similar failure behaviors, which can be explained and predicted by this model. The pulsed DC lifetime is found to be longer than DC lifetime, and the AC lifetime is found to be very much longer. This recognition can provide significant relief to circuit designs involving metals carrying pulsed DC and AC currents, and allow a more aggressive design to improve circuit density and speed