氩激光治疗血管瘤疗效观察

血管瘤临床较常见，治疗手段多种，如冷冻、放射、手术及硬化剂注射等，但疗效均不甚满意。作者自1984年11月至1986年5月，用氩激光治疗血管瘤51例，随访6月至2年，疗效尚可。现报导如下：     

Tuning Garwick's algorithm for repacking sequential storage

Garwick's algorithm, for repacking LIFO lists stored in a contiguous block of memory, bases the allocation of remaining space upon both sharing and previous stack growth. A system whereby the weight applied to each method can be adjusted according to the current behavior of the stacks is discussed.We also investigate the problem of determining during memory repacking that the memory is used to saturation and the driving program should therefore be aborted. The tuning parameters studied here seem to offer no new grasp on this problem.     

Carbon-13 spin–lattice relaxation in strongly associated molecules: Carboxylic acids

¹³C spin–lattice relaxation times determined for the protonated carbons of carboxylic acids and methyl esters give indications of solution dimerization with the free acids. Since isopthalic and fumaric acids have two carboxyl functions they are able to polymerize in solution. Unlike the case for molecular aggregation due to weak hydrogen bonding in solution (e.g. alcohols, phenols), the ¹³C T₁ values of mono carboxylic acids are not significantly affected by dilution to c. 10^?2 M. Variable temperature T₁ measurements of both the mono and dibasic acids gave activation energies for molecular reorientation of the order of 2 kcal mol^?1, considerably lower than E_a for hydrogen bonded alcohols and comparable with E_a for the unassociated methyl esters of propionic and benzoic acids.     

Quantum dots-based reverse phase protein microarray

CdSe nanocrystals, also called quantum dots (Qdots) are a novel class of fluorophores, which have a diameter of a few nanometers and possess high quantum yield, tunable emission wavelength and photostability. They are an attractive alternative to conventional fluorescent dyes. Quantum dots can be silanized to be soluble in aqueous solution under biological conditions, and thus be used in bio-detection. In this study, we established a novel Qdot-based technology platform that can perform accurate and reproducible quantification of protein concentration in a crude cell lysate background. Protein lysates have been spiked with a target protein, and a dilution series of the cell lysate with a dynamic range of three orders of magnitude has been used for this proof-of-concept study. The dilution series has been spotted in microarray format, and protein detection has been achieved with a sensitivity that is at least comparable to standard commercial assays, which are based on horseradish peroxidase (HRP)-catalyzed diaminobenzidine (DAB) chromogenesis. The data obtained through the Qdot method has shown a close linear correlation between relative fluorescence unit and relative protein concentration. The Qdot results are in almost complete agreement with data we obtained with the well-established HRP-DAB colorimetric array (R² = 0.986). This suggests that Qdots can be used for protein quantification in microarray format, using the platform presented here.     

Novel electrophoresis mechanism based on synchronous alternating drag perturbation

We present a novel means of transporting molecules in solution by applying a zero-time-average alternating motive force to the molecules, and perturbing the molecular drag coefficient synchronously with the applied force, thus causing a net drift in a direction determined by the phase of the alternating drag perturbation relative to the alternating force. We apply an electrophoretic form of the method to transport and concentrate DNA in a gel, such that all molecules migrate on average away from the nearest electrode and toward a central region. Since an electrode does not occupy this central region, this method presents the possibility of transporting and focusing DNA and other charged molecules in regions free from electrodes and the associated electrochemistry.     

A cyclic hexapeptide comprising alternating alpha-aminoxy and alpha-amino acids is a selective chloride ion receptor

In nonpolar solvents, the cyclic hexapeptide 2, which comprises alternating D-alpha-amino and D-alpha-aminoxy acids, adopts a C3-symmetric conformation with alternating eight (N--O turns)- and seven (gamma turns)-membered-ring hydrogen bonds. A series of anion-binding studies has suggested that 2 can function as an effective anion receptor that not only displays a high selectivity for chloride ions, but also the capability to extract chloride ions from aqueous solutions into organic phases.     

Mass spectrometric studies of the formation and reactivity of trans-[PtCl2(Am)(piperidinopiperidine)] x HCl complexes with ubiquitin

trans-[PtCl2(Am)(pip-pip)] x HCl complexes, where Am = ammine, methylamine and dimethylamine, react with ubiquitin to form 1:1 covalent adducts. The platinum complexes bind exclusively to Met1 of ubiquitin forming trans-[PtCl(S-Met1-Ub)(Am)(pip-pip)] adducts. These adducts are reactive towards nucleophiles and react with deoxyguanosine (dGMP) to form the ternary trans-[Pt(dGMP)(S-Met1-Ub) (Am)(pip-pip)] complex which is stable in water and even in the presence of excess glutathione (GSH). Reaction of trans-[PtCl(S-Met1-Ub)(Am)(pip-pip)] with GSH resulted in the rapid formation of the ternary complex trans-[Pt(GS)(S-Met1-Ub)(Am)(pip-pip)] which was not stable and slowly lost the platinum moiety; after 7 days the platinum moiety was completely removed and the native ubiquitin was regenerated.     

Liquid-core capsules via interfacial polymerization: a free-radical analogy of the nylon rope trick

Liquid-core capsules have wide-ranging applications in the high-efficiency encapsulation and controlled release of drugs, dyes, enzymes, and other substrates. Their great utility has driven the rapid development of various preparation techniques. However, there remains no convenient technique for the preparation of submicrometer liquid-core capsules with shell thicknesses less than 100 nm. Here, we demonstrate a new interfacial free-radical polymerization approach for the straightforward preparation of liquid-core polymer capsules. Conceptually, this interfacial free-radical polymerization is analogous to the classical "nylon rope trick" wherein hydrophobic and hydrophilic monomers alternately copolymerize to constrain the polymerization at interfaces, but its free-radical mechanism allows precise control of initiation, which makes it possible to finely disperse the immiscible phases prior to polymerization.