Co-C bond dissociation energy and reaction volume change of 2',5'-dideoxyadenosylcobalamin studied by laser-induced time-resolved photoacoustic calorimetry

Time resolved photoacoustic calorimetry (PAC) was applied to a study of the photolysis of a coenzyme B(12) analog 2',5'-dideoxyadenosylcobalamin, which lacks an -OH group at the 2' position of ribofuranose ring. In aqueous solution, we report for the first time the quantum yield Phi(d) (0.25+/-0.02), Co-C bond dissociation energy (BDE; 31.8+/-2.5 kcal mol(-1)) and reaction volume change deltaV(R) (6.5+/-0.5 ml mol(-1)) due to conformation changes of the corrin ring and its side chains accompanying the cleavage of the Co-C bond. These values for the analog are very similar to those for the natural cofactor. Based our results and previous studies, a possible explanation for the similarity in their structure and properties versus the large difference in their enzymatic activity is discussed.     

Chiral separation of amino acids derivatized with fluoresceine-5isothiocyanate by capillary electrophoresis and laser-induced fluorescence detection using mixed selectors of beta-cyclodextrin and sodium taurocholate

Chiral separation of 20 pairs of amino acids derivatized with fluoresceine-5-isothiocyanate (FITC) by capillary electrophoresis and laser-induced fluorescence detection was studied using the mixture of beta-cyclodextrin (beta-CD) and sodium taurocholate (STC) as selector. Resolution was considerably superior to that obtained by using either beta-CD or STC alone. The molar ratio of beta-CD to STC of about 2:3 was found to be critical to achieve maximum separation. At this beta-CD-to-STC ratio, chiral separation occurred at really low total concentration of beta-CD and STC (<0.1 mM). Other impacting factors were investigated including the total concentration of beta-CD and STC, pH, and capillary conditioning procedure between two successive runs. Using a running buffer of 80 mM borate containing 20 mM beta-CD and 30 mM STC at pH 9.3, all of the 20 pairs of FITC-amino acid enantiomers were baseline resolved. The resolutions of the most pairs of the amino acid enantiomers (17 of 20) were higher than 3.0, only three pairs gave a resolution lower than 3.0 but higher than 1.90 (beta-phenylserine, pSer). The highest resolution reached 14.58 (Glu). Two derivatives of beta-CD, 2-hydroxypropyl-beta-CD (HP-beta-CD) and heptakis(2,6-di-O-methyl)-beta-CD (DM-beta-CD) were also explored. HP-beta-CD showed similar cooperative effect with STC, while DM-beta-CD together with STC led to poorer chiral separation.     

Electrostatic layer-by-layer assembled carbon nanotube multilayer film and its electrocatalytic activity for O2 reduction

Multilayer films of shortened multiwalled carbon nanotubes (MWNTs) are homogeneously and stably assembled on glassy carbon electrodes with the layer-by-layer (LBL) method, based on electrostatic interaction of positively charged poly(diallyldimethylammonium chloride) and negatively charged and shortened MWNTs. The film assembly and electrochemical property as well as the electrocatalytic activity toward O2 reduction of the MWNT multilayer film are studied. Scanning electron microscopy, the quartz crystal microbalance technique, ultraviolet-visible-near-infrared spectroscopy, and cyclic voltammetry are used for characterization of film assembly. Experimental results revealed that film growth is uniform, almost with the same coverage of the MWNTs in each layer, and that the assembled MWNTs are mainly in the form of small bundles or single tubes on the electrodes. Electrochemical studies indicate that the LBL assembled MWNT films possess a remarkable electrocatalytic activity toward O2 reduction in alkaline media. This property, combined with the well-dispersed, porous and conductive features of the MWNT film illustrated with the LBL method, suggests the potential application of the MWNT film for constructing an efficient alkaline air electrode for energy conversions.     

环形腔高掺Er3 窄线宽光纤激光器实验研究

报道了采用光纤光栅（FBG）短直腔选频结构的环形腔窄线宽光纤激光器。采用约3m长高掺Er^3光纤，LD抽运阈值功率约为11mW，在25mW976nm有效抽运功率时输出信号功率为2．65mW，斜效率约为15％；输出激光3dB线宽小于0．01nm，20dB线宽小于0．04nm，边模抑制比（SMSR）为48dB；观察到输出信号光波长漂移范围为0．06nm。     

现代工业设计中的新技术应用

科学技术的迅速发展支撑了现代工业设计的进步,针对现代工业设计的核心内容-产品设计的流程,讨论了各种新技术在产品设计各阶段中的应用,重点分析了计算机辅助工业设计(CAID)技术的应用现状及未来发展趋势.     

基于SPCE061A单片机的髋作用力测试仪设计

介绍了基于SPCE061A单片机的髋作用力测试仪的系统结构及部分软件编写流程,着重分析了测试仪的测力方法以及使用光电编码器进行测速和鉴相的原理.测试仪以SPCE061A单片机为控制核心,配有串行通信口,具备上位机实时监控、数据保存和复现、测试数据离线分析等功能.实验证明,该测试仪具有测量准确、稳定性高及操控界面友好等优点.     

Coverage by randomly deployed wireless sensor networks

One of the main applications of wireless sensor networks is to provide proper coverage of their deployment regions. A wireless sensor network k-covers its deployment region if every point in its deployment region is within the coverage ranges of at least k sensors. In this paper, we assume that the sensors are deployed as either a Poisson point process or a uniform point process in a square or disk region, and study how the probability of the k-coverage changes with the sensing radius or the number of sensors. Our results take the complicated boundary effect into account, rather than avoiding it by assuming the toroidal metric as done in the literature.     

Symbol error rate of selection amplify-and-forward relay systems

Cooperative diversity schemes significantly improve the performance of wireless networks by transmitting the same information through several nodes. The amplify-and-forward (AF) relaying method is one of the most attractive cooperative diversity schemes due to its low complexity. Selection AF relaying has recently been proven to achieve the same diversity order as and lower outage probability than all-participate relays. In this letter, we present an asymptotic analysis of the symbol error rates of a selection AF network, and compare it with the conventional all-participate scheme     

Low-temperature conductance measurements of surface states in HfO2–Si structures with different gate materials

Metal-oxide-semiconductor capacitors based on HfO₂ gate stack with different metal and metal compound gates (Al, TiN, NiSi and NiAlN) are compared to study the effect of the gate electrode material on the trap density at the insulator–semiconductor interface.C–V and G–ω measurements were made in the frequency range from 1 kHz to 1 MHz in the temperature range 180–300 K. From the maximum of the plot G/ω vs. ln(ω) the density of interface states was calculated, and from its position on the frequency axis the trap cross-section was found. Reducing temperature makes it possible to decrease leakage current through the dielectric and to investigate the states located closer to the band edge.The structures under study were shown to contain significant interface trap densities located near the valence band edge (around 2×10¹¹ cm⁻²eV⁻¹ for Al and up to (3.5–5.5)×10¹² cm⁻² eV⁻¹ for other gate materials). The peak in the surface state distribution is situated at 0.18 eV above the valence band edge for Al electrode. The capture cross-section is 5.8×10⁻¹⁷ cm² at 200 K for Al–HfO₂–Si structure.