Juan Luis Villanueva仿真软件在S7-200 PLC教学中的应用

为了改善PLC课堂的教学效果,将Juan Luis Villanueva仿真软件用于S7-200PLC课程的日常教学。首先对Juan Luis Villanueva仿真软件做了简单介绍,之后通过实现四台电机顺序启动同时停止的例子介绍了使用仿真软件的教学过程,最后简要说明了采用仿真软件在PLC教学中的优势。     

用户的候鸟效应对移动通信网络的冲击

随着经济和社会的发展,人们的流动性越来越强,运营商的用户、移动终端也在同步漫游迁移。在3G时代,由于智能终端的大量普及和用户使用习惯的改变,这种大规模的用户迁移对网络产生了较大的冲击。深入分析了候鸟用户的组成和使用习惯,聚焦于农民工和学生2个候鸟用户群体,并通过中国联通2个地（市）春节前后网络数据的对比,得出了候鸟用户对人口流出城市和人口流入城市移动通信网络的不同影响,并提出了运营商的应对策略,即网络建设中充分考虑漫游用户数量,修正用户预测模型,在人口流入地区与人口流出地区采用灵活的资源共享机制。     

基于自适应衰减因子Kalman 滤波的GPS 相位平滑伪距算法

载波相位平滑伪距的主要目的是通过高精度的载波相位测量值作为辅助量,使伪距测量值中、大随机误差得以消减。针对GPS 伪距测量中未知时变的噪声,提出基于极大后验时变噪声统计估计器的自适应衰减因子Kalman 滤波算法(AFKF),采用衰减的加权因子,使估计器逐渐忘记陈旧数据的作用,同时增加新数据的比重,避免滤波过程的发散。结合载波相位平滑伪距原理,利用AFKF 算法对全球导航卫星系统(GNSS)的国际GNSS 服务组织(IGS)的跟踪站实测数据进行仿真分析,并提出利用伪距双差值及伪距三差值来直观体现不同算法的效果比较,结果表明:与标准Kalman 滤波相比,AFKF 算法在伪距平滑应用中取得很好的效果。     

pH‐ and NIR Light‐Responsive Micelles with Hyperthermia‐Triggered Tumor Penetration and Cytoplasm Drug Release to Reverse Doxorubicin Resistance in Breast Cancer

The acquisition of multidrug resistance (MDR) is a major hurdle for the successful chemotherapy of tumors. Herein, a novel hybrid micelle with pH and near‐infrared (NIR) light dual‐responsive property is reported for reversing doxorubicin (DOX) resistance in breast cancer. The hybrid micelles are designed to integrate the pH‐ and NIR light‐responsive property of an amphiphilic diblock polymer and the high DOX loading capacity of a polymeric prodrug into one single nanocomposite. At physiological condition (i.e., pH 7.4), the micelles form compact nanostructure with particle size around 30 nm to facilitate blood circulation and passive tumor targeting. Meanwhile, the micelles are quickly dissociated in weakly acidic environment (i.e., pH ≤ 6.2) to release DOX prodrug. When exposed to NIR laser irradiation, the hybrid micelles can trigger notable tumor penetration and cytosol release of DOX payload by inducing tunable hyperthermia effect. In combination with localized NIR laser irradiation, the hybrid micelles significantly inhibit the growth of DOX‐resistant MCF‐7/ADR breast cancer in an orthotopic tumor bearing mouse model. Taken together, this pH and NIR light‐responsive micelles with hyperthermia‐triggered tumor penetration and cytoplasm drug release can be an effective nanoplatform to combat cancer MDR.     

Complementary MS Methods Assist Conformational Characterization of Antibodies with Altered S–S Bonding Networks

As therapeutic monoclonal antibodies (mAbs) become a major focus in biotechnology and a source of the next-generation drugs, new analytical methods or combination methods are needed for monitoring changes in higher order structure and effects of post-translational modifications. The complexity of these molecules and their vulnerability to structural change provide a serious challenge. We describe here the use of complementary mass spectrometry methods that not only characterize mutant mAbs but also may provide a general framework for characterizing higher order structure of other protein therapeutics and biosimilars. To frame the challenge, we selected members of the IgG2 subclass that have distinct disulfide isomeric structures as a model to evaluate an overall approach that uses ion mobility, top-down MS sequencing, and protein footprinting in the form of fast photochemical oxidation of proteins (FPOP). These three methods are rapid, sensitive, respond to subtle changes in conformation of Cys?→?Ser mutants of an IgG2, each representing a single disulfide isoform, and may be used in series to probe higher order structure. The outcome suggests that this approach of using various methods in combination can assist the development and quality control of protein therapeutics.      

Synthesis, structure, and activity evaluation of two silver(I) complexes as Helicobacter pylori urease inhibitors

Two silver(I) compounds, [Ag(R,R-hxn)](C₇H₄BrO₂) · 2H₂O (I) (Chxn = 1,2-diaminocyclohexane) and [Ag(C₅H₆N₂)₂]₂(C₈H₄O₄) · 10H₂O (II), were synthesized and complex I was structurally characterized by X-ray crystallography. Compound I contains a catena-(trans-1,2-diaminocyclohexane) silver polycation ([Ag(Chxn)]_∞) in a roughly linear fashion, while II possesses a linear-type silver monocation. Compounds I and II were evaluated for their inhibitory activities against Helicobacter pylori urease in vitro. Both were found to have strong inhibitory activities against H. pylori urease comparable to that of acetohydroxamic acid.     

Biochemical and Structural Studies of Conserved Maf Proteins Revealed Nucleotide Pyrophosphatases with a Preference for Modified Nucleotides

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Study on the structure and reactivity of COREX coal

COREX is the primary process in the current smelting reduction method. The process has strict coal quality standards. Combustion processes of coal used in the COREX operating system were analyzed using a synchronous thermogravimetric analyzer combined with a mass spectrometer. The microcosmic structure and macerals were observed by an electronic scanning microscope. The qualitative and quantitative determinations of oxygen functional groups, such as phenolic hydroxyl, carboxyl, carbonyl, and methoxy groups were detected by the Fourier Transform Infrared spectrometer (FT-IR) and through chemical analysis methods. In addition, the evolution of the chemical structure and transformation mechanism of organic oxygen functional groups during COREX coal combustion have been thoroughly investigated. This study proposes a new coal-requirement index system and coal blending method, which will increase the expansion of coal selection and decrease the overall usage of coal during COREX.     

Preparation and characterization of an active catalyst

As an active catalyst to promote thermolysis of ammonium perchlorate (AP), potassium lead hexanitrocobaltate(II) complex (K₂Pb[Co(NO₂)₆]) was synthesized by the direct deposition method and inverse microemulsion method. Its submicron, size, cube morphology, and crystal structure were investigated by SEM, TEM, and XRD analysis, respectively. Thermal decomposition of K₂Pb[Co(NO₂)₆] was studied by the TG/DSC-IR online system and XRD analysis. The catalyst was decomposed at about 300 °C; its gaseous products were NO₂, NO, and N₂O and its solid products were Pb₃O₄, Co₃O₄, PbO, CoO, and KNO₂. Because thermal decomposition of the catalyst was synchronous with low temperature decomposition of AP, thermolysis of AP was promoted remarkably. In particular, the gaseous products (NO _x ) could directly oxidize the absorbed NH₃. As a result, compared to the data of pure AP, the integral heat of AP added 3.0 wt% of the catalyst multiplied by 280 %, the maximum rate of heat release increased by 634 %. The decomposition of catalyzed AP ended at about 317 °C, at which only less than 30 % of pure AP decomposed.