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11.
Jiao Xia Zou Wisely Chua Dr. Zheng Ser Shi Mei Wang Grace Shu Hui Chiang Kavitha Sanmugam Boon Yeow Tan Dr. Radoslaw M. Sobota Prof. Dr. Hao Li 《Angewandte Chemie (International ed. in English)》2023,62(33):e202307553
Ceramidases (CDases) are important in controlling skin barrier integrity by regulating ceramide composition and affording downstream signal molecules. While the functions of epidermal CDases are known, roles of neutral CDases secreted by skin-residing microbes are undefined. Here, we developed a one-step fluorogenic substrate, S-B , for specific detection of bacterial CDase activity and inhibitor screening. We identified a non-hydrolyzable substrate mimic, C6 , as the best hit. Based on C6 , we designed a photoaffinity probe, JX-1 , which efficiently detects bacterial CDases. Using JX-1 , we identified endogenous low-abundance PaCDase in a P. aeruginosa monoculture and in a mixed skin bacteria culture. Harnessing both S-B and JX-1 , we found that CDase activity positively correlates with the relative abundance of P. aeruginosa and is negatively associated with wound area reduction in clinical diabetic foot ulcer patient samples. Overall, our study demonstrates that bacterial CDases are important regulators of skin ceramides and potentially play a role in wound healing. 相似文献
12.
Chua Hong Tan Kok N. Cheung Montgomery W. L. 《Applied biochemistry and biotechnology》1996,57(1):851-856
Applied Biochemistry and Biotechnology - Bulking and foaming in activated sludge have been associated with filamentous overgrowth. FilamentousNocardia amarae and nonfilamentousPseudomonas... 相似文献
13.
Wong Mui F. Chua Hong Lo Waihung Leung Chu K. Yu Peter H. F. 《Applied biochemistry and biotechnology》2001,91(1-9):447-457
Studies were conducted toinvestigate the removal and recovery of copper (II) ions from aqueous solutions by Micrococcus sp., which was isolated from a local activated sludge process. The equilibrium of copper biosorption followed the Langmuir
isotherm model very well with a maximum biosorption capacity (qmax) of 36.5 mg of Cu2+/gofdry cell at pH 5.0 and 52.1 mg of Cu2+/g of dry cell at pH 6.0. Cells harvested at exponential growth phase and stationary phase showed similar biosorption characteristics
for copper, Copper uptake by cells was negligible at pH 2.0 and then increased rapidly with increasing pH un til 6.0. In multim
etal systems, Micrococcus sp. exhibited a preferential biosorption order: Cu−Pb>Ni−Zn. There is virtually no interference with copper uptake by Micrococcus sp. from solutions bearing high concentrations of Cl−, SO
4
2−
, and NO3/− (0–500 mg/L). Sulfuric acid (0.05 M) was the most efficient desorption medium, recovering >90% of the initial
copper sorbed. The copper capacity of Micrococcus sp. remained unchanged after five successive sorption and desorption cycles. Immobilization of Micrococcus sp. in 2% calcium alginate and 10% polyacrylamide gel beads increased copper uptake by 61%. Biomass of Micrococcus sp. may be applicable to the development of potentially cost-effective biosorbent for removing and recovering copper from
effluents. 相似文献
14.
15.
Z. B. Yan F. Duan T. N. Wong K. C. Toh K. F. Choo P. K. Chan Y. S. Chua L. W. Lee 《Heat and Mass Transfer》2013,49(7):985-990
An inclined spray chamber with four multiple nozzles to cool a 1 kW 6U electronic test card has been designed and tested in this study. The multiple inclined sprays can cover the same heated surface area as that with the multiple normal sprays but halve the volume of the spray chamber. The spray cooling system used R134a as a working fluid in a modified refrigeration cycle. It is observed that increasing mass flow rate and pressure drop across the nozzles improved the heat transfer coefficient with a maximum enhancement of 117 %, and reduced the maximum temperature difference at the heated surface from 13.8 to 8.4 °C in the inclined spray chamber with a heat flux of 5.25 W/cm2, while the heat transfer coefficient of the normal spray increased with a maximum enhancement of 215 % and the maximum temperature difference decreased from 10.8 to 5.4 °C under similar operating conditions. We conclude that the multiple inclined sprays could produce a higher heat transfer coefficient but with an increase in non-uniformity of the surface temperature compared with the multiple normal sprays. 相似文献
16.
17.
Di Ventra M. Pershin Y.V. Chua L.O. 《Proceedings of the IEEE. Institute of Electrical and Electronics Engineers》2009,97(10):1717-1724
We extend the notion of memristive systems to capacitive and inductive elements, namely, capacitors and inductors whose properties depend on the state and history of the system. All these elements typically show pinched hysteretic loops in the two constitutive variables that define them: current-voltage for the memristor, charge-voltage for the memcapacitor, and current-flux for the meminductor. We argue that these devices are common at the nanoscale, where the dynamical properties of electrons and ions are likely to depend on the history of the system, at least within certain time scales. These elements and their combination in circuits open up new functionalities in electronics and are likely to find applications in neuromorphic devices to simulate learning, adaptive, and spontaneous behavior. 相似文献
18.
In the last few years, semiconductor random-access memory (RAM) components have been introduced into high-speed scratchpad applications and small buffer memory systems, where the performance or cost advantage of semiconductor components is greatest. More recently, developments in the technology of large-scale integrated (LSI) circuits have resulted in cost-performance characteristics of semiconductor RAMs that are competitive in computer main-frame memories as well. This article reviews the basic circuit concepts used in these components, representative products that are presently available, and some systems considerations involved in their use. 相似文献
19.
Two recent experimental developments, when combined, may have far-reaching implications. S(phiK(S))<0, if confirmed, would imply large s-b mixing, a new CP phase, and right-handed dynamics. Large Deltam(B(s)) would be likely, making the B(s) program at hadron machines difficult. Reconstruction of B vertex from K(S) at B factories, as shown by BaBar's first measurement of SK(S)(pi(0)), makes SK(S)(pi(0)gamma) in B-->K(*0)gamma accessible. This would be a boon for B factory upgrades. Supersymmetric Abelian flavor symmetry, independently motivated, can realize all of this with a light sb(1) squark. B factory and collider studies of flavor, CP and supersymmetry, may not be what we had expected. 相似文献
20.
The detection of TNT and related nitroaromatic compounds in seawater is of great interest. Electrochemical techniques can be applied for detection purposes since nitroaromatic compounds contain easily reducible nitro groups. In this study, we investigate the performance of thermally reduced graphenes prepared by three different oxidative methods: Hummers, Staudenmaier and Hofmann methods with consequent thermal exfoliation. The Hofmann method‐based graphene was found to exhibit the highest sensitivity in detecting TNT electrochemically. Extended study on the detection of TNT in seawater using the graphene material provided significant improvements in the detection sensitivity. These findings will have profound impacts on the detection of nitroaromatic explosives in seawater. 相似文献