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71.
Charalambous C.A. Cotton I. Aylott P. 《Vehicular Technology, IEEE Transactions on》2008,57(3):1404-1416
The production of stray currents by DC light rail systems leads to the corrosion of the supporting and third-party infrastructure in close proximity to the rail system. This paper simulates two parallel tracks that are occupied by two trains: one on each track. This type of modeling constitutes a case study that is utilized to investigate the effect of soil topologies on the corrosion performance of a floating DC light rail system focusing on the supporting and third-party infrastructure. The modeling technique used involves the accurate computation of the shunt and series parameters for use in a resistive-type model using a commercially available software package. The results demonstrate the importance that soil resistivity has on the corrosion risk to traction system and third-party infrastructure. Such information could ultimately be used to vary the level of stray current protection across a light rail system to ensure a consistent lifetime across the whole system. 相似文献
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74.
Divakar U Drew AJ Lee SL Gilardi R Mesot J Ogrin FY Charalambous D Forgan EM Menon GI Momono N Oda M Dewhurst CD Baines C 《Physical review letters》2004,92(23):237004
The order of the vortex state in La1.9Sr0.1CuO4 is probed using muon-spin rotation and small-angle neutron scattering. A transition from a Bragg glass to a vortex glass is observed, where the latter is composed of disordered vortex lines. In the vicinity of the transition the microscopic behavior reflects a delicate interplay of thermally induced and pinning-induced disorder. 相似文献
75.
Drew AJ Lee SL Charalambous D Potenza A Marrows C Luetkens H Suter A Prokscha T Khasanov R Morenzoni E Ucko D Forgan EM 《Physical review letters》2005,95(19):197201
Superconducting and magnetic order are usually mutually exclusive, and are found to coexist in relatively few materials. We have obtained direct evidence for a spin-density wave (SDW) coexisting with bulk superconductivity in a ferromagnetic-superconducting trilayer. In the superconducting state the amplitude of the SDW is enhanced and modeling the data also suggests a pi/2 phase shift of one component of the SDW, implying a profound coupling of these two forms of order. 相似文献
76.
Pautrat A Goupil C Simon Ch Charalambous D Forgan EM Lazard G Mathieu P Brûlet A 《Physical review letters》2003,90(8):087002
We report small-angle neutron scattering measurements on the vortex lattice in a PbIn polycrystal in the presence of an applied current. Using the rocking curves as a probe of the distribution of current in the sample, we observe that vortex pinning is due to the surface roughness. This leads to a surface current that persists in the flux-flow region. We show the influence of surface treatments on the distribution of this current. 相似文献
77.
C. G. R. Barrett J. Charalambous R. G. Copperthwaite M. J. Frazer 《Journal of mass spectrometry : JMS》1975,10(2):146-154
The relative intensities of peaks in the mass spectra of the compounds MX4?noxn (oxH = 8-quinolinol; n = 2; M = Ti; X = F, Cl, Br or OEt; M = Sn; X = F, Cl, Br or I; M = Ge; X = Cl or Br; n = 1; M = Ti; X = OEt) depend on the insertion temperature and the residence time of the sample in the mass spectrometer. In most cases ions which cannot arise by fragmentation of the respective molecular ions are observed. These ions arise from the ionisation and fragmentation of species which are due to redistribution reactions in the mass spectrometer. The fragmentation pattern of the compounds MX2ox2 (X = halogen), mainly involving loss of ligand radicals, is related to the common oxidation states of the metals and reflects the metal-halogen bond strength. The molecular ions of the compounds Ti(OEt)4?noxn (n = 0, 1 or 2) fragment by loss of intact ligand radicals. 相似文献
78.
Background
Reversible phosphorylation events within a polymerisation complex have been proposed to modulate capsular polysaccharide synthesis in Streptococcus pneumoniae. Similar phosphatase and kinase genes are present in the exopolysaccharide (EPS) biosynthesis loci of numerous lactic acid bacteria genomes.Results
The protein sequence deduced from the wzb gene in Lactobacillus rhamnosus ATCC 9595 reveals four motifs of the polymerase and histidinol phosphatase (PHP) superfamily of prokaryotic O-phosphatases. Native and modified His-tag fusion Wzb proteins were purified from Escherichia coli cultures. Extracts showed phosphatase activity towards tyrosine-containing peptides. The purified fusion protein Wzb was active on p-nitrophenyl-phosphate (pNPP), with an optimal activity in presence of bovine serum albumin (BSA 1%) at pH 7.3 and a temperature of 75°C. At 50°C, residual activity decreased to 10 %. Copper ions were essential for phosphatase activity, which was significantly increased by addition of cobalt. Mutated fusion Wzb proteins exhibited reduced phosphatase activity on p-nitrophenyl-phosphate. However, one variant (C6S) showed close to 20% increase in phosphatase activity.Conclusion
These characteristics reveal significant differences with the manganese-dependent CpsB protein tyrosine phosphatase described for Streptococcus pneumoniae as well as with the polysaccharide-related phosphatases of Gram negative bacteria. 相似文献79.
Charalambous K Booth PJ Woscholski R Seddon JM Templer RH Law RV Barter LM Ces O 《Journal of the American Chemical Society》2012,134(13):5746-5749
Mechanical properties of biological membranes are known to regulate membrane protein function. Despite this, current models of protein communication typically feature only direct protein-protein or protein-small molecule interactions. Here we show for the first time that, by harnessing nanoscale mechanical energy within biological membranes, it is possible to promote controlled communication between proteins. By coupling lipid-protein modules and matching their response to the mechanical properties of the membrane, we have shown that the action of phospholipase A(2) on acyl-based phospholipids triggers the opening of the mechanosensitive channel, MscL, by generating membrane asymmetry. Our findings confirm that the global physical properties of biological membranes can act as information pathways between proteins, a novel mechanism of membrane-mediated protein-protein communication that has important implications for (i) the underlying structure of signaling pathways, (ii) our understanding of in vivo communication networks, and (iii) the generation of building blocks for artificial protein networks. 相似文献
80.
Despina Charalambous Nicolas-George Homer Eliades Michalis Christoforou Eleni Kakouri Charalabos Kanakis Petros A. Tarantilis Maria Pantelidou 《Molecules (Basel, Switzerland)》2022,27(9)
This study aimed to determine the chemical composition of different types of tissue of Cedrus brevifolia Henry (Pinaceae) methanolic extracts, namely needles, twigs, branches, and bark. Cedrus brevifolia is a narrow endemic coniferous tree species of Cyprus, growing in a sole population in the mountainous area of Paphos Forest. Chemical analysis of the extracts was performed using liquid chromatography combined with time-of-flight high-resolution mass spectrometry (LC/Q-TOF/HRMS). The majority of the 36 compounds tentatively identified belonged to the flavonoids family. The extract of needles was the richest extract in terms of secondary metabolites. The extracts were studied for their antioxidant activity using the DPPH free radical scavenging assay. Additionally, the antibacterial activity was evaluated by determining both the minimum inhibitory concentration and the minimum bactericidal concentration against Staphylococcus aureus and Escherichia coli. All extracts demonstrated antioxidant property, while bark gave the highest antioxidant capacity (IC50 value of 0.011 mg/mL) compared to the other tissues. Antibacterial activity was observed against both types of bacteria, with the extract of branches presenting the strongest activity against S. aureus (MIC, 0.097 mg/mL and MBC, 0.195 mg/mL). This is the first time that extracts of needles, twigs, branches, and bark of C. brevifolia are compared regarding their chemical composition as well as their antimicrobial and antioxidant properties. 相似文献