Gold nanoparticles-based colorimetric investigation of triplex formation under weak alkalic pH environment with the aid of Ag+

A novel colorimetric method for investigating triplex formation between oligonucleotide modified Au nanoparticles (AuNPs) under weak alkalic pH environment is developed based upon the specific recognition property of Ag+ with CGC triads. Oligonucleotide 5'-SH-T12-CTTCTTTCCTTTCTTC-3' (oligo-1) is modified on the surface of AuNPs. Upon addition of oligonucleotide 5'-GAAGAAAGGAAAGAAG-3' (oligo-2), triplex formation between oligo-1 modified AuNPs occurred at pH 8.0 with the aid of Ag+, triggers the aggregation of AuNPs, accompany with the solution color change from red to purple. The melting temperature demonstrates a 31 °C increase for the triplex DNA compose of 10 T?A°T triads and 6 C?G°C triads upon addition of Ag+, the disassociation constant (Kd) between Ag+ and C?G°C triads is 3.6 μM. Moreover, triplex formation between AuNPs depending on Ag+ can be used to recognize Ag+ ion with the naked eye, as well as UV-vis absorption spectroscopy.     

GAE-AFS法分析藏药当佐中总汞与人工胃液中的游离态汞

藏药中的重金属汞已成为藏药安全性评价研究的重点.该文以藏药当佐为研究对象,分别采用湿法消解和人工胃液处理,利用金汞齐富集-冷原子荧光法(GAE-AFS),测定当佐中总汞含量及其在人工胃液中游离态汞含量,并对所建市的方法及仪器的工作条件进行了考察.在选定的测试条件下,仪器系统稳定性良好,HgS的回收率(n=6)为99.5...     

湿法消解-FAAS测定4省区藏药南寒水石中的钙及5种微量元素

使用湿法消解-FAAS测定4省区（西藏、青海、甘肃、四川）藏药南寒水石中钙及5种微量元素铁、锰、锌、铜、镁的含量。采用空气-乙炔火焰原子吸收光谱法对微量元素进行测定。该方法快速、准确,以期所测数据为藏药南寒水石实际用药及其质量标准的制定提供参考。     

Perturbations of May–Leonard system

In this paper we study the quadratic homogeneous perturbations of the 3-dimensional May–Leonard system with α+β=2$\alpha +\beta =2$. It is shown that there are perturbed systems having exactly one or two limit cycles bifurcated from the periodic orbits of May–Leonard system. This is proved by estimating the number of zeros of the first and the second order Melnikov functions.     

BiTiO3电子结构及光学性质的第一性原理研究

采用基于第一性原理的赝势平面波方法, 对BiTiO₃的多种结构进行了计算. 计算结果表明, C1C1结构最为稳定, 对应晶格参数为a=b=5.606 Å, c=9.954 Å; α=β=105.1°, γ=61.2°. 进一步对C1C1结构的BiTiO₃的能带结构、电子性质和光学性质进行了研究, 发现BiTiO₃是间接带隙半导体, 其费米面附近的能带主要由Ti-3d和O-2p层的电子态构成. 通过介电函数、复折射率和反射率等的研究, 发现BiTiO₃的光学性质为近各向同性.     

Dynamic-light-scattering-based sequence-specific recognition of double-stranded DNA with oligonucleotide-functionalized gold nanoparticles

An ultrasensitive and simple dynamic-light-scattering (DLS) assay for the sequence-specific recognition of double-stranded DNA (dsDNA) was developed based on detection of the average diameter change of Au nanoparticle (AuNP) probes modified with oligonucleotides 5'-TTTCTCTTCCTT- CTCTTC-(T)(12)-SH-3' (Oligo 1) and 5'-TTCTTTCTTTTCTTTTTC-(T)(12)- SH-3' (Oligo 2). The target dsDNA was composed of two complementary oligonucleotides: 5'-AAAGAGAAGGAAGAGAAGAAGAAAGAAAAGAAAAAG-3' (Oligo 3) and 3'-TTTCTCTTCCTTCTCTTCTTCTTTCTTTTCTTTTTC-5' (Oligo 4). Hybridization of the two AuNPs-Oligo probes with the target dsDNA induced aggregation of the target dsDNA by forming triplex DNA, which accordingly increased the average diameter. This diameter change could then be detected by DLS. The average diameter was proportional to the target dsDNA concentration over the range from 593 fM to 40 pM, with a detection limit of 593 fM. Moreover, the assay had good sequence specificity for the target dsDNA.     

Electromagnetic field produced in high-energy small collision systems within charge density models of nucleons

Recent experiments show that \begin{document}$ \Delta\gamma $\end{document}, an observable designed to detect the chiral magnetic effect (CME), in small collision systems (\begin{document}$ p+A $\end{document}) is similar to that in heavy ion collisions (\begin{document}$ A+A $\end{document}). This introduces a challenge to the existence of the CME because it is believed that no azimuthal correlation exists between the orientation of the magnetic field (\begin{document}$ \Phi_B $\end{document}) and participant plane (\begin{document}$ \Phi_2 $\end{document}) in small collision systems. In this work, we introduce three charge density models to describe the inner charge distributions of protons and neutrons and calculate the electric and magnetic fields produced in small \begin{document}$ p+A $\end{document} collisions at both RHIC and LHC energies. Our results show that the contribution of the single projectile proton is the main contributor to the magnetic field after averaging over all participants. The azimuthal correlation between \begin{document}$ \Phi_B $\end{document} and \begin{document}$ \Phi_2 $\end{document} is small but not vanished. Additionally, owing to the large fluctuation in field strength, the magnetic-field contribution to \begin{document}$ \Delta\gamma $\end{document} may be large.     

Probing top-quark operators with precision electroweak measurements

In the standard model effective field theory, operators involving the top quark are generally difficult to probe and can generate sizable loop contributions to electroweak precision observables measured by past and future lepton colliders. Could the high precision of electroweak measurements compensate for loop suppression and provide competitive reaches on these operators? Would the inclusion of these contributions introduce too many additional parameters for a meaningful global electroweak analysis to be performed? In this paper, we perform a detailed phenomenological study to address these two important questions. Focusing on eight dimension-6 operators that generate anomalous couplings between electroweak gauge bosons and third-generation quarks, we calculate their one loop contributions to \begin{document}$ e^+e^- \to f\bar{f} $\end{document} processes, both on and off the Z-pole, and the \begin{document}$ e^-e^+ \to WW $\end{document} process. A global analysis is performed with these eight operators and those that contribute to the above processes at tree level using measurements at the LEP, SLC, and several low energy experiments. We find that although current electroweak precision measurements are sensitive to the one-loop effects of top-quark operators, it is difficult to separate them from the operators that contribute at tree level, making a global analysis rather challenging. Under further assumptions (for instance, new physics contributes to only third generation quark operators and the S and T parameters), competitive reaches may be obtained in a global fit. Another important finding of our study is that the two operators that generate the dipole interactions of the bottom quark have a significant impact on the Z-pole measurements and should not be omitted. We also discuss the implications of the recently reported W-boson mass measurement at the CDF for our results. Finally, we estimate the reaches of future lepton colliders in probing top-quark operators with precision electroweak measurements.     

一种激光外差多普勒玻璃厚度测量方法

提出了一种应用激光外差测量技术结合激光多普勒技术的玻璃厚度测量方法.论述了该方法的理论依据,并结合实际应用介绍了外围硬件电路的设计方法,运用Matlab软件结合FFT技术进行了数值仿真实验,结果表明该方法在测量1~10 mm的平板玻璃时,误差小于1%.