Selective and Wash‐Resistant Fluorescent Dihydrocodeinone Derivatives Allow Single‐Molecule Imaging of μ‐Opioid Receptor Dimerization

μ‐Opioid receptors (μ‐ORs) play a critical role in the modulation of pain and mediate the effects of the most powerful analgesic drugs. Despite extensive efforts, it remains insufficiently understood how μ‐ORs produce specific effects in living cells. We developed new fluorescent ligands based on the μ‐OR antagonist E‐p‐nitrocinnamoylamino‐dihydrocodeinone (CACO), that display high affinity, long residence time and pronounced selectivity. Using these ligands, we achieved single‐molecule imaging of μ‐ORs on the surface of living cells at physiological expression levels. Our results reveal a high heterogeneity in the diffusion of μ‐ORs, with a relevant immobile fraction. Using a pair of fluorescent ligands of different color, we provide evidence that μ‐ORs interact with each other to form short‐lived homodimers on the plasma membrane. This approach provides a new strategy to investigate μ‐OR pharmacology at single‐molecule level.     

Determination of polychlorinated biphenyls by liquid chromatography–atmospheric pressure photoionization–mass spectrometry

This study presents the atmospheric pressure photoionization (APPI) of high‐chlorinated (five or more chlorine atoms) polychlorinated biphenyls (PCBs) using toluene as dopant, after liquid chromatographic separation. Mass spectra of PCB 101, 118, 138, 153, 180, 199, 206 and 209 were recorded by using liquid chromatography‐APPI‐tandem mass spectrometry (LC‐APPI‐MS/MS) in negative ion full scan mode. Intense peaks appeared at m/z that correspond to [M ? Cl + O]^? ions, where M is the analyte molecule. Furthermore, a detailed strategy, which includes designs of experiments, for the development and optimization of LC‐APPI‐MS/MS methods is described. Following this strategy, a sensitive and accurate method with low instrumental limits of detection, ranging from 0.29 pg for PCB 209 to 8.3 pg for PCB 101 on column, was developed. For the separation of the analytes, a Waters XSELECT HSS T3 (100 mm × 2.1 mm, 2.5 µm) column was used with methanol/water as elution system. This method was applied for the determination of the above PCBs in water samples (surface water, tap water and treated wastewater). For the extraction of PCBs from water samples, a simple liquid–liquid extraction with dichloromethane was used. Method limits of quantification, ranged from 4.8 ng l^?1, for PCB 199, to 9.4 ng l^?1, for PCB 180, and the recoveries ranged from 73%, for PCB 101, to 96%, for PCB 199. The estimated analytical figures were appropriate for trace analysis of high‐chlorinated PCBs in real samples. Copyright © 2014 John Wiley & Sons, Ltd.     

Complex frequency shifted convolution PML for FDTD modelling of elastic waves

The perfectly matched layer (PML) is nowadays considered as the best optimum absorbing boundary condition available. However, the PML with the classical stretching tensor has certain limitations. Strangely, these limitations have rarely been addressed in elastic wave modelling. For example, substantial reflections occur when strong evanescent waves are propagating parallel to the interface. To circumvent problems like this, the complex frequency shifted stretching tensor has been introduced in electromagnetic modelling. In this paper we show that the convolution PML with this stretching tensor as used in electromagnetic modelling can be adapted for elastic wave modelling. Numerical results of a model where the presence of evanescent waves is predominant show that the PML based on the complex frequency shifted stretching tensor can improve the performance of the absorbing boundary layer considerably.     

Δ(1232) axial charge and form factors from lattice QCD

We present the first calculation on the Δ axial?vector and pseudoscalar form factors using lattice QCD. Two Goldberger-Treiman relations are derived and examined. A combined chiral fit is performed to the nucleon axial charge, N to Δ axial transition coupling constant and Δ axial charge.     

How Big Is Too Big? Critical Shocks for Systemic Failure Cascades

External or internal shocks may lead to the collapse of a system consisting of many agents. If the shock hits only one agent initially and causes it to fail, this can induce a cascade of failures among neighboring agents. Several critical constellations determine whether this cascade affects the system in part or as a whole which, in the second case, leads to systemic risk. We investigate the critical parameters for such cascades in a simple model, where agents are characterized by an individual threshold θ _i determining their capacity to handle a load αθ _i with 1?α being their safety margin. If agents fail, they redistribute their load equally to K neighboring agents in a regular network. For three different threshold distributions P(θ), we derive analytical results for the size of the cascade, X(t), which is regarded as a measure of systemic risk, and the time when it stops. We focus on two different regimes, (i) EEE, an external extreme event where the size of the shock is of the order of the total capacity of the network, and (ii) RIE, a random internal event where the size of the shock is of the order of the capacity of an agent. We find that even for large extreme events that exceed the capacity of the network finite cascades are still possible, if a power-law threshold distribution is assumed. On the other hand, even small random fluctuations may lead to full cascades if critical conditions are met. Most importantly, we demonstrate that the size of the “big” shock is not the problem, as the systemic risk only varies slightly for changes in the number of initially failing agents, the safety margin and the threshold distribution, which further gives hints on how to reduce systemic risk.     

Position Tracking for Passive UHF RFID Tags with the Aid of a Scanned Array

Thanks to the proliferation of radio frequency identification systems (RFID), applications have emerged concerning positioning techniques for inexpensive passive RFID tags. The most accurate approaches for tracking the tag’s position, deliver precision in the order of 20 cm over a range of a few meters and require moving parts in a predefined pattern (mechanical antenna steering), which limits their application. Herein, we introduce an RFID tag positioning system that utilizes an active electronically-steered array, based on the principles of modern radar systems. We thoroughly examine and present the main attributes of the system with the aid of an finite element method simulation model and investigate the system performance with far-field tests. The demonstrated positioning precision of 1.5°, which translates to under 1 cm laterally for a range of a few meters can be helpful in applications like mobile robot localization and the automated handling of packaged goods.     

Discrete Finite-Element Simulation of Thermoelectric Phenomena in Spark Plasma Sintering

Realistic microstructures of compacted powders formed by spark plasma sintering or field-activated sintering technology were modeled using the discrete finite-element method. Two key thermoelectric characteristics were studied: (1) the effect of the electric current pattern, i.e., direct current (DC) and pulsed current, on temperature distributions in the compacted powders, and (2) the effect of compaction modes, i.e., isostatic compaction and uniaxial compaction, on conductivity. Simulations showed that, for the same electric power input, pulsed current offered faster heating and more uniform temperature distribution in the compact than did DC. Additionally, using uniaxial compaction, the effective conductivity of the compact in the compaction direction was higher than in the transverse direction, by as much as 20%. Experimental measurements confirmed the existence of anisotropy of conductivity in the compact.     

Quasiparticlelike peaks, kinks, and electron-phonon coupling at the (pi,0) regions in the CMR oxide La2-2x Sr1+2x Mn2 O7

Using angle-resolved photoemission, we have observed sharp quasiparticlelike peaks in the prototypical layered manganite La(2-2x)Sr(1+2x)Mn(2)O(7) (x=0.36,0.38). We focus on the (pi,0) regions of k space and study their electronic scattering rates and dispersion kinks, uncovering bilayer-split bands, the critical energy scales, momentum scales, and strengths of the interactions that renormalize the electrons. To identify these bosons, we measured phonon dispersions in the energy range of the kink by inelastic neutron scattering, finding a good match in both energy and momentum to the oxygen bond-stretching phonons.     

The group of isometries of a locally compact metric space with one end

In this note we study the dynamics of the natural evaluation action of the group of isometries G of a locally compact metric space (X,d) with one end. Using the notion of pseudo-components introduced by S. Gao and A.S. Kechris we show that X has only finitely many pseudo-components exactly one of which is not compact and G acts properly on this pseudo-component. The complement of the non-compact component is a compact subset of X and G may fail to act properly on it.     

Cost analysis and efficient radio bearer selection for multicasting over UMTS

Along with the widespread deployment of the Third Generation (3G) cellular networks, the fast‐improving capabilities of the mobile devices, content, and service providers are increasingly interested in supporting multicast communications over wireless networks and in particular over Universal Mobile Telecommunications System (UMTS). To this direction, the Third Generation Partnership Project (3GPP) is currently standardizing the Multimedia Broadcast/Multicast Service (MBMS) framework of UMTS. In this paper, we present an overview of the MBMS multicast mode of UMTS. We analytically present the multicast mode of the MBMS and analyze its performance in terms of packet delivery cost under various network topologies, cell types, and multicast users' distributions. Furthermore, for the evaluation of the scheme, we consider different transport channels for the transmission of the multicast data over the UMTS Terrestrial Radio‐Access Network (UTRAN) interfaces. Finally, we propose a scheme for the efficient radio bearer selection that minimizes total packet delivery cost. Copyright © 2008 John Wiley & Sons, Ltd.