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61.
Popović DM Zmirić A Zarić SD Knapp EW 《Journal of the American Chemical Society》2002,124(14):3775-3782
Charge separation and radical transfer in DNA photolyase from Escherichia coli is investigated by computing electrostatic free energies from a solution of the Poisson-Boltzmann equation. For the initial charge separation 450 meV are available. According to recent experiments [Aubert et al. Nature 2000, 405, 586-590] the flavin receives an electron from the proximal tryptophan W382, which consequently forms a cationic radical WH(*)(+)382. The radical state is subsequently transferred along the triad W382-W359-W306 of conserved tryptophans. The radical transfer to the intermediate tryptophan W359 is nearly isoenergetic (58 meV uphill); the radical transfer from the intermediate W359 to the distal W306 is 200 meV downhill in energy, funneling and stabilizing the radical state at W306. The resulting cationic radical WH(*)(+)306 is further stabilized by deprotonation, yielding the neutral radical W(*)306, which is 214 meV below WH(*)(+)306. The time scale of the charge recombination process yielding back the resting enzyme with FADH(*) is governed by reprotonation of W306, with a calculated lifetime of 1.2 ms that correlates well with the measured lifetime of 17 ms. In photolyase from Anacystis nidulans the radical state is partially transferred to a tyrosine [Aubert et al. Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 5423-5427]. In photolyase from Escherichia coli, there is a tyrosine (Y464) close to the distal tryptophan W306 that could play this role. We show that this tyrosine cannot be involved in radical transfer, because the electron transfer from tyrosine to W306 is much too endergonic (750 meV) and a direct hydrogen transfer is likely too slow. Coupling of specific charge states of the tryptophan triad with protonation patterns of titratable residues of photolyase is small. 相似文献
62.
Adaptive perceptual color-texture image segmentation. 总被引:2,自引:0,他引:2
Junqing Chen Thrasyvoulos N Pappas Aleksandra Mojsilovi? Bernice E Rogowitz 《IEEE transactions on image processing》2005,14(10):1524-1536
We propose a new approach for image segmentation that is based on low-level features for color and texture. It is aimed at segmentation of natural scenes, in which the color and texture of each segment does not typically exhibit uniform statistical characteristics. The proposed approach combines knowledge of human perception with an understanding of signal characteristics in order to segment natural scenes into perceptually/semantically uniform regions. The proposed approach is based on two types of spatially adaptive low-level features. The first describes the local color composition in terms of spatially adaptive dominant colors, and the second describes the spatial characteristics of the grayscale component of the texture. Together, they provide a simple and effective characterization of texture that the proposed algorithm uses to obtain robust and, at the same time, accurate and precise segmentations. The resulting segmentations convey semantic information that can be used for content-based retrieval. The performance of the proposed algorithms is demonstrated in the domain of photographic images, including low-resolution, degraded, and compressed images. 相似文献
63.
Sebastian Mendrek Aleksandra Mendrek Hans-Juergen Adler Andrzej Dworak Dirk Kuckling 《Colloid and polymer science》2010,288(7):777-786
Temperature-sensitive poly(glycidol)-b-poly(N-isopropylacrylamide) block copolymers (PGl55PNIPAAmy) were synthesised and their aqueous solutions investigated by different methods including differential scanning calorimetry
(DSC), UV-VIS spectroscopy as well as dynamic and static light scattering. The cloud point temperature (T
c) depended on the composition of the investigated block copolymers and increased with decreasing length of the PNIPAAm block
in PGl55PNIPAAmy copolymers. In contrast, the enthalpy of phase separation of PNIPAAm segments measured by DSC decreased with decreasing length
of the PNIPAAm block in the polymer. These findings can be correlated with the behaviour of homo-PNIPAAm with similar molecular
weights indicating that the influence of PGl on the local environment and phase separation of PNIPAAm chains is similar to
the influence observed for PNIPAAm chains bearing different low molecular weight end group. Using DLS measurement, it was
shown that the aggregation process depended on the PGl/PNIPAAm block ratio. If the PGl/PNIPAAm ratio was low, stable core-shell
aggregates were formed. In contrast, the tendency to formation of large unstable, loose aggregates was observed for copolymers
with high PGl/PNIPAAm ratio. 相似文献
64.
Luca Sancineto Carmine Ostacolo David Ortega-Alarcon Ana Jimenez-Alesanco Laura Ceballos-Laita Sonia Vega Olga Abian Adrian Velazquez-Campoy Silvia Moretti Agnieszka Dabrowska Pawel Botwina Aleksandra Synowiec Anna Kula-Pacurar Krzysztof Pyrc Nunzio Iraci Claudio Santi 《Molecules (Basel, Switzerland)》2021,26(19)
The COVID-19 pandemic outbreak prompts an urgent need for efficient therapeutics, and repurposing of known drugs has been extensively used in an attempt to get to anti-SARS-CoV-2 agents in the shortest possible time. The glycoside rutin shows manifold pharmacological activities and, despite its use being limited by its poor solubility in water, it is the active principle of many pharmaceutical preparations. We herein report our in silico and experimental investigations of rutin as a SARS-CoV-2 Mpro inhibitor and of its water solubility improvement obtained by mixing it with l-arginine. Tests of the rutin/l-arginine mixture in a cellular model of SARS-CoV-2 infection highlighted that the mixture still suffers from unfavorable pharmacokinetic properties, but nonetheless, the results of this study suggest that rutin might be a good starting point for hit optimization. 相似文献
65.
Paweł Berczyński Aleksandra Kładna Irena Kruk Teresa Piechowska Hassan Y. Aboul-Enein Oya Bozdağ-Dündar Meltem Ceylan-Unlusoy 《Journal of fluorescence》2013,23(6):1319-1327
The antioxidant properties of 11 new synthesized chromonyl-2,4-thiazolidinediones and chromonyl-2,4-imidazolidinediones (CBs) were investigated. The antioxidant activities and mechanisms of the CBs interaction with reactive oxygen species (ROS) were clarified using various in vitro antioxidant assay methods including superoxide anion radical ( $ \mathrm{O}\overline{{}_2^{\bullet }} $ ), hydroxyl radical (HO?), 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH?) scavenging activity and the iron (II)-ferrozine complex formation. The potassium superoxide/18-crown-6 ether dissolved in dimethylsulfoxide (DMSO) was applied as a source of superoxide anion radical. Hydroxyl radicals were produced in the Fenton-like reaction Fe(II)+H2O2. Chemiluminescence, spectrophotometry, and electron paramagnetic resonance (EPR) spectroscopy using 5,5-dimethyl-1-pyrroline-1-oxide (DMPO) as spin trap were applied as the measurement techniques. The CBs examined that exhibited good free radical scavenging activity also showed strong total antioxidant power capacity. Possible mechanisms of antioxidation are proposed to explain the differences in the experimental results between the chromone derivatives with imidazolidine-2,4-dione ring and those with thiazolidine-2,4-dione ring. In conclusion, some of the new CBs are promising to be applied as inhibitors of free radicals. 相似文献
66.
MITIC Snezana ZIVANOVIC Valentina OBRADOVIC Mirjana TOSIC Snezana PAVLOVIC Aleksandra 《中国化学》2007,25(4):531-534
The kinetic method for the determination of phosphate microamounts was described. The developed method is based on catalytic effect of phosphate on sodium pyrogallol-5-sulphonate (PS) by dissolved oxygen. The reaction was followed spectrophotometrically by measuring the rate of change in the values of the absorbance of the oxidation product at 437 nm. The optimum reaction conditions are PS (0.44×10^-3 mol·L^-1) and HClO4 (3.6×10^-6 mol·L^-1) at 25 ℃. Following this procedure, phosphate can be determined with a linear calibration graph up to 0.23 μg·mL^-1. The interference effect of several species was also investigated and it was found that the most common cations and anions did not interfere with the determination. The developed procedure was successfully applied to the determination of phosphate in natural waters and soil. 相似文献
67.
Aleksandra M. Korostyshevskaya 《Applied magnetic resonance》2011,41(2-4):537-542
Magnetic resonance imaging (MRI) abilities in pre- and postnatal monitoring of cytomegalovirus-related brain lesions from early prenatal period to infancy are described. Objective of our study is to illustrate the gradual evolution of intracranial abnormalities in a single patient through serial pre- and postnatal MRI. It demonstrates the progress of a cerebral cytomegalovirus infection from fetal to postnatal life with the lack of concordance between prenatal ultrasonography and MRI, and proves the great importance of MRI, being in most cases the only tool for early detection and monitoring of this potentially devastating entity. It was shown that the additional postnatal MRI increases the possibilities in the specific characterization of lesions revealed in the fetus brain, which account for a wide spectrum of different sequences and best spatial resolution of postnatal MRI in comparison with the prenatal one. 相似文献
68.
Sebastian Mendrek Aleksandra Mendrek Hans‐Juergen Adler Andrzej Dworak Dirk Kuckling 《Journal of polymer science. Part A, Polymer chemistry》2009,47(7):1782-1794
Block copolymers of poly(glycidol)‐b‐poly(4‐vinylpyridine) were obtained by ATRP of 4‐vinylpyridine initiated by ω‐(2‐chloropropionyl) poly(glycidol) macroinitiators. By changing the monomer/macroinitiator ratio in the synthesis polymers with varied P4VP/PGl molar ratio were obtained. The obtained block copolymers showed pH sensitive solubility. It was found that the linkage of a hydrophilic poly(glycidol) block to a P4VP influenced the pKa value of P4VP. DLS measurements showed the formation of fully collapsed aggregates exceeding pH 4.7. Above this pH values the collapsed P4VP core of the aggregates was stabilized by a surrounding hydrophilic poly(glycidol) corona. The size of the aggregates depended significantly upon the composition of the block copolymers. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 1782–1794, 2009 相似文献
69.
Lung transplantation is a necessary step for the patients with the end stage of chronic obstructive pulmonary disease. The use of artificial lungs is a promising alternative to natural lung transplantation which is complicated and is restricted by low organ donations. For successful lung engineering, it is important to choose the correct combination of specific biological cells and a synthetic carrier polymer. The focus of this study was to investigate the interactions of human lung epithelial cell line NCl-H292 that is involved in lung tissue development with the biodegradable poly(ε-caprolactone) before and after its chemical modification to evaluate potential for use in artificial lung formation. Also, the effect of polymer chemical modification on its mechanical and surface properties has been investigated. The poly(ε-caprolactone) surface was modified using aminolysis followed by immobilization of gelatin. The unmodified and modified polymer surfaces were characterized for roughness, tensile strength, and NCl-H292 metabolic cell activity. The results showed for the first time the possibility for NCI-H292 cells to adhere on this polymeric material. The resazurin assay showed that the metabolic activity at 24?h postseeding of 80% in the presence of the unmodified and greater than 100% in the presence of the modified polymer was observed. The roughness of the poly(ε-caprolactone) increased from 4 to 26?nm and the film strength increased from 0.01 to 0.045 kN when the material was chemically modified. The results obtained to date show potential for using modified poly(ε-caprolactone) as a scaffold for lung tissue engineering. 相似文献
70.
Aleksandra Zivkovic 《Tetrahedron letters》2010,51(29):3769-3771
Fingolimod (FTY720) and its analogue derivatives are not only promising therapeutics in sphingolipid signaling but also valuable tools for understanding the roles of sphingolipids in (patho)physiological conditions. A practical method for the synthesis of the ether analogue of FTY720 is described. Our final synthetic approach allows high yield and efficient synthesis of O-FTY in only four steps without chromatographic purifications. 相似文献