Fungus-mediated biotransformation of amorphous silica in rice husk to nanocrystalline silica

Rice husk is a cheap agro-based waste material, which harbors a substantial amount of silica in the form of amorphous hydrated silica grains. However, there have been no attempts at harnessing the enormous amount of amorphous silica present in rice husk and its room-temperature biotransformation into crystalline silica nanoparticles. In this study, we address this issue and describe how naturally deposited amorphous biosilica in rice husk can be bioleached and simultaneously biotransformed into high value crystalline silica nanoparticles. We show here that the fungus Fusarium oxysporum rapidly biotransforms the naturally occurring amorphous plant biosilica into crystalline silica and leach out silica extracellularly at room temperature in the form of 2-6 nm quasi-spherical, highly crystalline silica nanoparticles capped by stabilizing proteins; that the nanoparticles are released into solution is an advantage of this process with significant application and commercial potential. Calcination of the silica nanoparticles leads to loss of occluded protein and to an apparently porous structure often of cubic morphology. The room-temperature synthesis of oxide nanomaterials using microorganisms starting from potential cheap agro-industrial waste materials is an exciting possibility and could lead to an energy-conserving and economically viable green approach toward the large-scale synthesis of oxide nanomaterials.     

An optical biosensor for dichlovos using stacked sol-gel films containing acetylcholinesterase and a lipophilic chromoionophore

An optical biosensor consisting of a chromoionophore (ETH5294) (CM) doped sol-gel film interfaced with another sol-gel film immobilized with acetylcholinesterase (AChE) was employed to detect the insecticide dichlorvos. The main advantage of this optical biosensor is the use of a sol-gel layer with immobilized CM that possesses lipophilic property. The highly lipophilic nature of the CM and its compatibility with the sol-gel matrix has prevented leaching, which is frequently a problem in optical sensor construction based on pH indicator dyes. The immobilization of the indicator and enzyme was simple and need no chemical modification. The CM layer is pH sensitive and detects the pH changes of the acetylcholine chloride (AChCl) substrate when hydrolyzed by AChE layer deposited above. In the absence of the AChE layer, the pH response of the CM layer is linear from pH 6 to 8 (R² = 0.98, n = 3) and it showed no leaching of the lipophilic chromoionophore. When the AChE layer is deposited on top, the optical biosensor responds to AChCl with a linear dynamic range of 40-90 mM AChCl (R² = 0.984, n = 6). The response time of the biosensor is 12 min. Based on the optimum incubation time of 15 min, a linear calibration curve of dichlorvos against the percentage inhibition of AChE was obtained from 0.5 to 7 mg/L of dichlorvos (17-85% inhibition, R² = 0.991, n = 9). The detection limit for dichlorvos was 0.5 mg/L. The results of the analysis of 1.7-6.0 mg/L of dichlorvos using this optical biosensor agreed well with a gas chromatography-mass spectrometry detection method.     

Error analysis for rotating active Stokes-Mueller imaging polarimeters

Retrieval of Mueller matrices from intensity measurements is a noise-sensitive process. In addition, the choice of the method used for extracting Mueller matrix elements greatly influences the precision of the final results. Among available procedures, three have been tested and their robustness analyzed by adding Gaussian noise to computer synthesized data and have been verified by comparing experimental data. As expected, the methods based on classical matrix inversion reach their noise reduction limit even if more data are considered. In contrast, the discrete Fourier transform approach features a remarkable stability. The identified reason is that the retrieval process corresponds to filtering with a periodic kernel.     

Real-time acoustic classification of sperm whale clicks and shipping impulses from deep-sea observatories

The automated real-time detection and classification of cetacean and anthropogenic sounds from deep-sea observatories can play a key role to study cetaceans in the field, to quantify the impact of anthropogenic sounds or to initiate mitigation measures during potentially harmful human activities. In the area of the NEMO-ONDE deep-sea observatory, sperm whales are often present together with heavy shipping. The spatial coincidence of both sound sources allows for the long term monitoring of their interaction. Some ships produce impulsive sounds and the automated separation of these impulses from sperm whale clicks is not a trivial task. As part of a detection, classification and localisation system for acoustic data from marine observatories, we present four modules performing the automated real-time classification of clicks from sperm whales and impulsive sounds produced by ships. First, two modules detect segments that contain impulsive sounds within a specifiable frequency band and return the impulses’ positions. Then, two modules classify the detected impulses as sperm whale clicks or ship impulses. Finally, at the level of 22 s segments, the classification outputs from individual impulses are combined into a decision on the presence of sperm whale clicks or ship impulses. The modules’ reliability was tested on data from the NEMO-ONDE observatory. Training and testing data were separated by more than 2 months, enabling to assess the consistency of the predictions over the long term. The automated separation between segments of the two classes was high with area under the ROC curve (AUC) values between 0.94 and 0.98.     

Nonclassical Symmetries for Nonlinear Partial Differential Equations via Compatibility

The determining equations for the nonclassical symmetry reductions of nonlinear partial differential equations with arbitrary order can be obtained by requiring the compatibility between the original equations and the invariant surface conditions. The (2+1)-dimensional shallow water wave equation,
Boussinesq equation, and the dispersive wave equations in shallow water serve as examples illustrating how compatibility leads quickly and easily to the determining equations for their nonclassical symmetries.     

Longitudinal and transverse structure functions in decaying nearly homogeneous and isotropic turbulence

Streamwise evolution of longitudinal and transverse velocity structure functions in a decaying homogeneous and nearly isotropic turbulence is reported for Reynolds numbers Reλ up to 720. First, two theoretical relations between longitudinal and transverse structure functions are examined in the light of recently derived relations and the results show that the low-order transverse structure functions can be well approximated by longitudinal ones within the sub-inertial range. Reconstruction of fourth-order transverse structure functions with a recently proposed relation by Grauer et al. is comparatively less valid than the relation already proposed by Antonia et al. Secondly, extended self-similarity methods are used to measure the scaling exponents up to order eight and the streamwise evolution of scaling exponents is explored. The scaling exponents of longitudinal structure functions are, at first location, close to Zybin’s model, and at the fourth location, close to She–Leveque model. No obvious trend is found for the streamwise evolution of longitudinal scaling exponents, whereas, on the contrary, transverse scaling exponents become slightly smaller with the development of a steamwise direction. Finally, the stremwise variation of the order-dependent isotropy ratio indicates the turbulence at the last location is closer to isotropic than the other three locations.     

General Formalism for the BRST Symmetry

In this paper we will discuss Faddeev-Popov method for gauge theories with a general form of gauge symmetry in an abstract way. We will then develope a general formalism for dealing with the BRST symmetry. This formalism will make it possible to analyse the BRST symmetry for any theory.     

Mixed Convection Boundary-Layer Flow Along a Vertical Cylinder Embedded in a Porous Medium Filled by a Nanofluid

The steady mixed convection boundary-layer flow on a vertical circular cylinder embedded in a porous medium filled by a nanofluid is studied for both cases of a heated and a cooled cylinder. The governing system of partial differential equations is reduced to ordinary differential equations by assuming that the surface temperature of the cylinder and the velocity of the external (inviscid) flow vary linearly with the axial distance x measured from the leading edge. Solutions of the resulting ordinary differential equations for the flow and heat transfer characteristics are evaluated numerically for various values of the governing parameters, namely the nanoparticle volume fraction ${\phi}$ , the mixed convection or buoyancy parameter ?? and the curvature parameter ??. Results are presented for the specific case of copper nanoparticles. A critical value ?? _c of ?? with ?? _c <?0 is found, with the values of | ?? _c| increasing as the curvature parameter ?? or nanoparticle volume fraction ${\phi}$ is increased. Dual solutions are seen for all values of ?? >??? _c for both aiding, ?? >?0 and opposing, ?? <?0, flows. Asymptotic solutions are also determined for both the free convection limit ${(\lambda \gg 1)}$ and for large curvature parameter ${(\gamma \gg 1)}$ .     

Dynamic analysis of a simply supported beam resting on a nonlinear elastic foundation under compressive axial load using nonlinear normal modes techniques under three-to-one internal resonance condition

In this paper, the Nonlinear Normal Modes (NNMs) analysis for the case of three-to-one (3:1) internal resonance of a slender simply supported beam in presence of compressive axial load resting on a nonlinear elastic foundation is studied. Using the Euler?CBernoulli beam model, the governing nonlinear PDE of the beam??s transverse vibration and also its associated boundary conditions are extracted. These nonlinear motion equation and boundary condition relations are solved simultaneously using four different approximate-analytical solution techniques, namely the method of Multiple Time Scales, the method of Normal Forms, the method of Shaw and Pierre, and the method of King and Vakakis. The obtained results at this stage using four different methods which are all in time?Cspace domain are compared and it is concluded that all the methods result in a similar answer for the amplitude part of the transverse vibration. At the next step, the nonlinear normal modes are obtained. Furthermore, the effect of axial compressive force in the dynamic analysis of such a beam is studied. Finally, under three-to-one-internal resonance condition the NNMs of the beam and the steady-state stability analysis are performed. Then the effect of changing the values of different parameters on the beam??s dynamic response is also considered. Moreover, 3-D plots of stability analysis in the steady-state condition and the beam??s amplitude frequency response curves are presented.     

Effects of tip gap and amplitude of piezoelectric fans on the performance of heat sinks in microelectronic cooling

Piezoelectric fan is a promising option for cooling microelectronic devices owing to its unique features such as no electromagnetic noise, low power consumption and minimum space requirement. The recent interest is to integrate the piezoelectric fans (piezofans) with heat sink; this idea is widely accepted and researches are still underway. This article presents experimental analysis on the effects of tip gap (δ) and amplitude of piezofan vibration (α) on the heat transfer characteristics of finned heat sinks. Two heat sink configurations, namely A and B (with two and four fins respectively) each of which is arranged with three piezofans, are considered for the study. The transient temperature distributions for cases with and without piezofans are obtained for both the configurations, and compared. The heat transfer coefficient, thermal impedance, Nusselt number and Reynolds number are investigated as functions of δ and α. The effect of α on the fan effectiveness is also analyzed. It is observed that the configuration B has better cooling performance compared to A. Among the tested ranges of δ and α, the case with least tip gap (δ?=?0.03) and highest amplitude (α?=?5.29) is found to be the best; at this setting, the fan effectiveness is increased to almost 4?times compared to the case without piezofans.