牛磺酸通过调控AKT/GSK-3β通路抑制结直肠癌细胞侵袭转移

观察糖原合酶激酶-3β(GSK-3β)在牛磺酸(Tau)抑制结直肠癌(CRC)侵袭转移中的作用,探讨Tau抑制结直肠癌侵袭转移分子机制。选用人结直肠癌SW480和HT29细胞为研究对象,用浓度为40～200 mmoL Tau处理细胞;Transwell小室和细胞划痕愈合实验检测细胞侵袭、迁移能力,Western blot检测细胞EMT标志性蛋白(E-cadherin, N-cadherin, Vimentin),基质金属蛋白酶2/7(MMP2/7)和AKT/GSK-3β通路相关蛋白水平。与正常对照组比较,Tau可浓度依赖性抑制CRC细胞侵袭和迁移,上调CRC上皮标志物E-cadherin表达,下调EMT间质标志物N-cadherin, Vimentin和MMPs表达;降低AKT和p-AKT水平,提高PTEN,GSK-3β及p-GSK-3β表达水平(P<0.05)。与p-EGFP-GSK-3β或Tau组比较,p-EGFP-GSK-3β+Tau组细胞迁移和侵袭数量有显著性降低(P<0.01);与Tau组或p-EGFP-GSK-3β组比较,p-EGFP-GSK-3β+Tau组E-Cadherin、GSK-3β蛋白表达有显著升高(P<0.01),而N-Cadherin, Vimentin,β-Catenin, MMP2/7和AKT蛋白表达均有显著降低(P<0.01)。牛磺酸可通过调控AKT/GSK-3β通路抑制结直肠癌细胞侵袭转移。     

Measurement of 129Xe frequency shift due to Cs–129Xe collisions

Enhancement factor K0, which characterizes NMR and EPR frequency shifts for Cs-129Xe, is measured for the first time. The enhancement factor r-o was measured to be （702±41） at 80 ℃ and （653±20） at 90 ℃, using the NMR frequency shift, detected by atomic magnetometer at a low magnetic field of 100 nT. This result is useful for predicting the EPR frequency shifts for Cs and the NMR frequency shifts for 129Xe in spin-exchange cells.     

投影壳模型研究丰中子奇奇核102,104Nb的转动能谱

运用投影壳模型研究了正常形变丰中子核同位素102,104Nb的低能级能谱, 并指定了它的准粒子组态, 理论计算所得到的能谱曲线与实验给出的能谱曲线非常一致, 说明了投影壳模型对研究重核低能级谱非常有效。 The Projected Shell Model(PSM) is used to study the low energy scheme of the neutron rich normal deformed isotopes of odd odd nuclei 102, 104Nb. The quasiparticle configuration is assigned. The theoretical calculations of the energy band of 102,104Nb could well reproduce the experimental data. It is shown that PSM is a valid method for studying the low energy scheme of heavy nuclei.     

r×t阶Kirkman三连系构造的一种方法

发现了 6n+3的高阶 Kirkman三连系构造方法 .阐明了 r× t阶 Kirkman三连系构造的基本理论 .给出了完全三分图的定义 ,并证明了关于 Kirkman三连系构造的命题 .介绍了 1 3 5阶 Kirkman三连系的构造过程 .     

Small Stress Change Triggering a Big Earthquake： a Test of the Critical Point Hypothesis for Earthquakes

Whether or not a small stress change can trigger a big earthquake is one of the most important problems related to the critical point hypothesis for earthquakes. We investigate global earthquakes with different focM mechanisms which have different levels of ambient shear stress. This ambient stress level is the stress level required by the earthquakes for their occurrence. Earthquake pairs are studied to see whether the occurrence of the preceding event encourages the occurrence of the succeeding one in terms of the Coulomb stress triggering. It is observed that the stress triggering effect produced by the change of Coulomb failure stress in the same order of magnitudes,about 10-2 MPa, is distinctly different for different focal mechanisms, and thus for different ambient stress levels.For non-strike-slip earthquakes with a relatively low ambient stress level, the triggering effect is more evident,while for strike-slip earthquakes with a relatively high ambient stress level, there is no evident triggering effect.This water level test provides an observational support to the critical point hypothesis for earthquakes.     

均三溴偶氮胂分光光度法测定植物体及种子中铈组稀土

随着稀土元素在农业上应用研究的深入,需要对种子、植株中微量铈组稀土进行测定。目前已有人用中子活化法^[1]萃淋树脂分离富集光度法^[2]进行测定。由于植物中钙等干扰元素的含量高(钙含量为0.1—5 mg/g),给直接光度法测定带来困难。     

固相萃取-气相色谱-串联质谱法同时测定武汉市普通人群血清中35种有机氯农药与多氯联苯

有机氯农药(OCPs)和多氯联苯(PCBs)是两类重要的持久性有机污染物,可在环境介质中长期存在,并通过多种途径进入人体,导致人体的高暴露风险。OCPs和PCBs对人体存在诸多健康危害,精准定量人体内OCPs和PCBs的暴露水平是健康效应评价的关键。该研究基于固相萃取-气相色谱-串联质谱联用技术(SPE-GC-MS/MS)建立了同时检测100 μL血清中35种OCPs和PCBs的分析方法。血清样品经尿素沉淀蛋白后,采用Oasis^® HLB小柱净化,正己烷-二氯甲烷混合溶液(1∶1, v/v)洗脱,氮吹近干,正己烷定容,多反应监测(MRM)模式检测,内标法定量分析。结果表明,OCPs和PCBs在0.05~50.0 ng/mL范围内线性关系良好,检出限在1.2~71.4 ng/L之间。35种目标分析物的加标回收率在72.6%~142%之间,相对标准偏差小于25%。利用所建立的方法检测了武汉市普通人群血清样本中OCPs和PCBs的浓度水平,结果表明武汉市普通人群广泛暴露于OCPs和PCBs,且以OCPs为主。有8种OCPs和7种PCBs检出率高于50%,其中p,p'-滴滴伊、p,p'-滴滴滴和甲氧滴滴涕检出率达100%,非类二噁英PCBs是PCBs的主要成分。血清中OCPs浓度随年龄增长呈升高趋势,在60岁以上存在性别差异;不同性别、年龄人群血清中PCBs浓度无统计学差异。该方法样本用量少,操作简便,具有较高的准确度和精密度,适用于环境健康研究中大量人群血清样本中痕量OCPs和PCBs的生物监测。     

Zn-Fe-SiO2复合镀层的制备工艺及其耐蚀性研究

在酸性硫酸盐体系中电沉积制备了Zn-Fe-SiO2复合镀层,并研究了工艺因素对镀层SiO2含量的影响.随着电流密度由4.8A/dm2增加到6A/dm2时,镀层中SiO2的含量由0.21%上升到0.47%,但是当电流密度继续增加到7.2A/dm2时,SiO2含量反而下降到了0.18%.当镀液中SiO2浓度由20g/L增加到60g/L时,镀层中SiO2含量由0.40%上升到0.51%.当镀液pH值由2增加到4时,镀层中SiO2含量由0.22%上升到1.17%.采用5%NaCl溶液浸泡试验考察了Zn镀层、Zn-Fe合金镀层与Zn-Fe-SiO2复合镀层的耐蚀性,并研究了镀层中SiO2含量对镀层耐蚀性的影响.结果表明,Zn-Fe-SiO2复合镀层无需钝化处理,其耐蚀性优于Zn镀层及Zn-Fe合金镀层,并且镀层的耐蚀性随着镀层中SiO2含量增大而提高.